ISSN:0012-2823    

DOI:10.1159/000193235

出版商:S. Karger AG    

年代:1913

数据来源: Karger

摘要:

Isolated acinar cells from rat pancreas responded well to hormonal treatment. Both secretin (synthetic and highly purified from porcine origin) and CCK-octapeptide stimulated amylase secretion in these cells. The response in both cases was very rapid. A maximal output of enzyme was reached within 5–lOmin after the addition of hormones. The concentration producing maximal output for synthetic secretin (Schwarz/Mann) was 5 X 10-8M, synthetic secretin (Squibb), 10-5M, for purified porcine secretin, 10-5M, and for CCK-octapeptide was 5 X 10-10M. Secretin (2-fold at optimal concentration) was found to be less efficient compared to CCK-octapeptide (5-fold at optimal concentration) in stimulating amylase release. A combination of secretin and CCK-octapeptide had a synergistic action in stimulating enzyme release by the acinar cells. In addition, pretreatment of acinar cells with secretin potentiated the secretory response of the treated cells to CCK-octapeptide. To a lesser extent pretreatment with CCK-octapeptide also increased the effect of secretin in stimulating enzyme secretio

ISSN:0012-2823    

DOI:10.1159/000198316

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Chronic drainage of pancreatic juice in rats results in a non-parallel secretion of digestive enzymes. The concentration of trypsinogen, chymotrypsinogen, procarboxypeptidase B and lipase increases continuously whereas that of amylase drops. An amino acid mixture given intravenously does not affect this dissociation. Reinfusion of bilio-pancreatic juice into the duodenum on the contrary influences the relative enzyme release: the increase in concentration of the three proteolytic enzymes and of lipase is less pronounced over the 72-hour study period; amylase concentration remains unaffected. In the absence of an intravenous amino acid feeding the pancreatic protein output is more than halved and a negative nitrogen balance is observed. Reinfusion of bilio-pancreatic juice does not alter the total protein output. This report confirms the persistence of a dissociation in the rate of enzyme release in rats with chronic pancreatic fistula despite sufficient protein administration and intestinal reintroduction of bilio-pancreatic juice.

ISSN:0012-2823    

DOI:10.1159/000198317

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000193236

出版商:S. Karger AG    

年代:1913

数据来源: Karger

摘要:

72 h after ligation or external fistulation of the common bile duct the activities of maltase, sucrase and lactase in the homogenate of the small intestinal mucosa of the rat were determined. The experiments were performed in connexion with intestinal perfusion studies, and the disaccharidase activities were measured in unperfused intestinal segments as well as in intestinal loops which had previously been perfused with a sucrose-containing solution. After bile duct ligation, the sucrase and maltase activities in a previously perfused intestinal loop were not different from those in sham-operated animals, the lactase activity was diminished. In a nonperfused segment, the sucrase activity was greater, the maltase activity was unchanged, and the lactase activity was lower than in control animals. After bile duct fistulation, the sucrase, maltase and lactase activities in a perfused segment were lower than in sham-operated rats. In a nonperfused loop, the sucrase activity was greater, the maltase activity was unchanged, and the lactase activity was lower then in the corresponding control group. These data suggest that bile is a factor which influences the total mucosal disaccharidase activities and, probably, the intracellular enzyme distribution.

ISSN:0012-2823    

DOI:10.1159/000198319

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000193237

出版商:S. Karger AG    

年代:1913

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000192955

出版商:S. Karger AG    

年代:1913

数据来源: Karger

摘要:

The activities of amylase, lipase, phospholipase and trypsin of twelve commercial pancreatic enzyme preparations were measured under identical conditions. Human small intestinal juice was chosen as incubation medium. A wide variation of enzymic activities was found in preparations in tablet form contrary to preparations in granulated form. A prerequisite for a successful therapy is enzymically potent pancreatic extracts with high enzyme content per tablet, capsule or recommended dose of granulated preparation. Therefore, some preparations seem to be preferable to others for clinical purposes.

ISSN:0012-2823    

DOI:10.1159/000198321

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

The parotid saliva test was evaluated in 31 patients with chronic pancreatitis proven by the secretin-pancreozymin test and failed to detect pancreatic insufficiency in the majority of the cases. According to this result the test cannot be recommended as a screening test for chronic pancreatitis.

ISSN:0012-2823    

DOI:10.1159/000198322

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000192956

出版商:S. Karger AG    

年代:1913

数据来源: Karger