摘要:

AbstractTo study the effect of protein synthesis inhibition on long‐term sensitization of the gill‐ and siphon‐withdrawal reflex ofAplysia, we have developed an isolated reflex preparation in which we could expose the inhibitor to only that part of the central nervous system involved in mediating the reflex and not to the other parts of the animal's central nervous system, thus minimizing the possible systemic side effects. We have found that long‐term sensitization can be obtained in the isolated gill reflex, and that this long‐term process, but not the short‐term process, is blocked selectively by anisomycin, a reversible inhibitor of protein synthesis. Moreover, to obtain this blockade of long‐term sensitization, this drug need only be applied during the train

ISSN:0022-3034    

DOI:10.1002/neu.480200102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractInjecting cGMP intoAplysianeuron R14 induced an inward current similar to one elicited by application of FMRFamide to the outside of that cell. In contrast, injection of cAMP into R14 caused a long‐lasting outward current and conductance increase. Phosphodiesterase inhibitors increased the cGMP and FMRFamide‐induced inward currents in R14. The cGMP‐induced inward current is voltage dependent and is largely carried by Na+. It is also strongly and inversely dependent on both external [Ca2+] and [Cl−], although these ions are not significant current carriers. Changing external [K+] had no effect. Voltage and ion dependencies of the cGMP‐induced inward current are similar to those of an inward current induced by FMRFamide. Thus cGMP may be a second messenger to FMRFamide in producing a slow inward current in R14. cGMP does not appear to be a second messenger to FMRFamide in mostAplys

ISSN:0022-3034    

DOI:10.1002/neu.480200103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThe marine molluscAplysia californicahas proved to be a useful preparation for analyzing the development of learning and memory on both behavioral and cellular levels. An important issue in this analysis concerns the anatomical substrate upon which learning is superimposed during development. As a first step in examining this question, in the present study we have determined the number of neurons in all the major central ganglia at each stage during juvenile development, a time when several forms of learning first emerge inAplysia. We found that a large and highly nonlinear proliferation of neurons occurs during juvenile development, with the greatest increase in cell number occurring during a specific juvenile stage: Stage 12. The neuronal proliferation is system‐wide, occurring in each of the central ganglia simultaneously, suggesting the action of a general developmental signal or trigger (perhaps a hormone). Accompanying the increase in neuron number in Stage 12 there is a large increase in neuropilar volume (150‐fold), which significantly increases the opportunity for synaptic interactions late in juvenile developm

ISSN:0022-3034    

DOI:10.1002/neu.480200104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

ISSN:0022-3034    

DOI:10.1002/neu.480200101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY