摘要:

ABSTRACT—By means of staining with Sirius Red F3BA in a saturated picric acid solution, the collagen contents of rat livers with varying degrees of fibrosis have been measured quantitatively in fixed and sectioned material, using both histophotometryin situand extraction of bound dye with colorimetric analysis. These findings have been correlated with chemical assays of the hydroxyproline content in homogenates from the same livers. It appears that a highly significant correlation exists between both section‐based analysis methods and the hydroxyproline content, the Spearman‐Rank correlation coefficients being virtually identical. For analysis of collagen accumulation in rat liver, both section‐based methods seem to be useful and reliable, the extraction method giving the quickest results for large‐scale screening, and the histophotometric method being more appropriate to take readings in selected areas. With human liver material, indications have been obtained for the existence of large sampling errors due to inhomogeneous distribution of collagen deposits. Using the extraction method, no significant changes could be observed in the volume density of collagen during postnatal growth from 1 week to 21 months in rat liver: only on the third day after birth was a higher value of collagen/total protein obtained, possibly due to a higher water content of the hepatocytes. Partial hepatectomy was found to have no influence at all on the collagen content of rat liver during the period of restorative growth or

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00428.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—Using Southern blot technique, the state of hepatitis B virus (HBV) DNA in liver tissue was investigated in 16 patients who were sero‐negative for hepatitis B surface antigen (HBsAg) but positive for its antibody (anti‐HBs). In only one case, was HBV DNA found in liver tissue in a heterogeneously integrated form. In this case, digestion with Taq I demonstrated integrated HBV DNA as two definite bands at 1.8 and 0.5 kbp. This suggests that HBV DNA in some cases persists even after HBV infection has been cleared serologically. It is possible that this persistence of HBV DNA plays an important role in hepatocarcinoge

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00429.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—Conflicting results have been common in the pharmacological treatments of portal hypertension. In an attempt to seek better management of portal hypertension, we studied the effect of the synthetic parathyroid hormone (PTH) fragment, [bPTH‐(1–34)], in portal hypertensive rats (partial portal vein ligation). PTH, 10 U/kg, administered via the jugular vein resulted in a reduction of both mean arterial blood pressure (MAP) and portal pressure (PP) to a similar extent (18.9% and 16.9%, respectively). A higher dose (40 U/kg) of PTH lowered the PP by 27.8% and MAP by 43.2%. Hemodynamic experiments, performed with labelled microspheres, demonstrated that PTH decreased the blood flow of the splanchnic and hepatic portal collateral vascular beds. To determine whether there is a direct vasodilatory effect on the venous vasculature, the effect of PTH on the isolated portal vein was examined. PTH was capable of inhibiting both spontaneous and drug (methacholine 10‐7mol/l or KC1 40 mmol/l‐induced contraction in a dose‐dependent manner. Therefore, it can be assumed that some of the effect of PTH on portal pressure is due to a selective effect on the

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00430.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—A morphologic study with immunohistochemical detection of the viral antigens of hepatitis B and Delta was performed on 36 liver specimens from patients with delta positive chronic viral hepatitis B. Electron microscopy was performed in 9 cases. No light microscopic or ultrastructural features specific for hepatitis D were observed. Lymphocytic infiltration occurred more often close to hepatocytes containing either hepatitis Delta antigen or hepatitis B core antigen in the cytoplasm, suggesting an involvement of cellular immune mechanisms in chronic hepatitis D as well as in hepatitis B. The simultaneous expression of both HBcAg and Delta antigen in occasional specimens over longer time periods indicates the possible co‐existence of both viruses without mutual inhibit

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00431.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—In order to characterize changes in bile acid profile during liver cell damage and regeneration, levels of bile acids in serum and bile were determined by high performance liquid chromatography (HPLC) in F344 rats treated with a single dose of D‐galactosamine (galactosamine, 300 mg/kg, i.p.) or subjected to two‐thirds partial hepatectomy (PH). In the serum, galactosamine caused elevation of conjugated bile acids such as taurocholic acid (TCA) and tauro‐ß‐muricholic acid (TßMCA) at the 24 and 48‐h time points, whereas unconjugated bile acids including cholic acid (CA) at 24 h and hyodeoxycholic acid (HDCA) at 48 h were increased after PH. In the bile, elevation of TCA showed most remarkable elevation at the 24‐h time point in the galactosamine‐treated group. All components of biliary bile acids showed rapid decreases from 24 to 48 h. The results demonstrated that while liver tissue damaged by galactosamine is able to conjugate bile acids it allows leakage into the blood stream. In contrast, the results for rats subjected to PH indicated that liver cells during DNA synthesis are not capable of conjugating all free bile acids with taurine although a similar leakage occurs. It is concluded that obvious elevation of serum TCA or CA and biliary TßMCA could be a useful indicator of hepatocell

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00432.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—Ketones can potentiate the hepatotoxicity of haloalkanes in animals. This may be due, in part, to changes in organelle susceptibility. Male Sprague‐Dawley rats were administered 15 mmol/kg (po) acetone, 2‐butanone, 2‐hexanone or 50 mg/kg (po) chlordecone or mirex (a nonketonic analog of chlordecone). Eighteen hours later, tests of organelle structure/function were performed (osmotic stress, respiration, and calcium pump activity). Other rats were given14CHCl3(0.5 or 1.0 ml/kg, po) 18 h after chlordecone or mirex administration. Three hours later, the organelle distribution of14C was evaluated. In a final experiment, ketone‐pretreated (chlordecone or 2‐hexanone) animals were killed 6 h after CHCl3administration and evaluated morphologically for evidence of modified organelle response. Acetone and chlordecone, when given alone, enhanced lysosomal fragility to osmotic stress; no changes in functional capacity of mitochondria or microsomes were observed. CHCl3‐derived14C in the mitochondrial fraction increased 2‐fold in chlordecone‐treated rats. Morphological evaluation suggested mitochondria respond differently to CHCl3in ketone‐pretreated (chlordecone or 2‐hexanone) animals compared to corn oil‐pretreated controls. These results support the concept that modifications of organelles contribute to the mechanism of ketone‐potentiation of CHC

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00433.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—A cluster of acute non‐A, non‐B hepatitis comprising 12 blood donors was diagnosed in a plasmapheresis unit. Nine cases were followed‐up for 2–5.5 years and seven out of them progressed to chronicity, as judged by biochemical abnormalities. In six, liver biopsy was performed 1 year after the acute disease revealing chronic active hepatitis in two, chronic persistent hepatitis in two, chronic lobular hepatitis in one and normal liver in one. Repeated biopsies showed progression to cirrhosis in one case of chronic active hepatitis, and resolution of the disease in another one, while in the remaining patients liver morphology remained unchanged. Circumstantial epidemiologic evidence suggests a single agent being the cause of the outbreak, which resulted in a broad spectrum of live

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00434.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—Reactivation of hepatitis B virus replication was investigated in an unselected group of 44 HBV DNA negative, anti‐HBe positive chronic HBsAg carriers. Twenty‐five patients (54%) were intravenous drug addicts and 7 (16%) were male homosexuals. Sixteen patients had evidence of delta infection and five of the seven male homosexuals had human immunodeficiency virus infection. The patients were followed for 1 to 180 months (median, 24 months) while HBV DNA negative, anti‐HBe positive. Reactivation, defined as reappearance of HBV DNA or HBeAg, or both, was detected in six patients corresponding to an annual reactivation rate of 5%. Reactivation in four patients was detected by reversion to HBV DNA positivity only, whereas HBeAg/anti‐HBe status remained unchanged. Two patients became both HBV DNA and HBeAg positive. None of the patients developed hepatitis‐like symptoms and transaminase elevation was only observed in two patients. Reactivation in two patients was ascribed to human immunodeficiency virus infection and in one patient to chronic lymphatic leukaemia. It is concluded that HBV DNA seems to be superior to HBeAg in the detection of reactivation of HBV replication and that reactivation associated with clinical symptoms leading to progression in chronic liver disease is a rare event in the populat

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00435.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

ABSTRACT—Immunohistochemistry with monoclonal anti‐cytokeratin antibodies has revealed the presence of cytokeratin 19 in embryonic and early fetal hepatocytes. With the differentiation of bile ducts at about the 10th week, cytokeratin 19 disappears from liver cells but remains in bile duct cells. This marks an important step in the organogenesis of the li

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00436.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00437.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY