摘要:

AbstractThe surface antigenic phenotype of peanut lectin agglutinin (PNA)‐separated, high‐density (“immature”) thymocytes which are the target for the T cell growth factor (TCGF) enabling this otherwise unresponsive cell population to mount a strong proliferative response to concanavalin A (Con A) has been studied. The results obtained indicate that (a) the major population of cells bearing the typical phenotype of immature thymocytes,i.e.low content of H‐2, high content of Thy‐1, TL, Lyt‐1 and Lyt‐2 antigens, is unable to respond to Con A in the presence of TCGF, and (b) that the responsive population constitutes a minor fraction (about 15%) of PNA+, high‐density thymocytes with surface antigenic phenotypes similar to those of mature T cells,i.e.high content of H‐2, low content of Thy‐1 and differentiated expression of Lyt antigens. Such a unique combination of properties and the presence of TL antigen on some members of the responding population suggest that it contains cells at intermediate stage(s) of differentiation between immature t

ISSN:0014-2980    

DOI:10.1002/eji.1830110102

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractPrevious studies in the mouse natural killer (NK) system have indicated that NK cells may be involved in lysing normal, primary hematopoietic tissues. In the present report, this was analyzed in the human NK system using fetal bone marrow (BM) cells and thymocytes as well as adult BM cells from healthy donors as target cells in a conventional 6 to 12‐h51Cr‐release assay.Adult BM cells showed low but significant levels of sensitivity, which could be increased by using interferon (IFN)‐activated peripheral blood leukocytes (PBL) as effector cells. BM cells from 16 to 19‐week‐old fetuses consistently showed higher sensitivity for lysis than adult BM, and also fetal thymocytes proved very sensitive for lysis, in contrast to what has previously been reported for adult thymocytes. When used as effector cells against K562 targets, adult BM showed a clear lytic activity which could be further activated with IFN. In contrast, fetal BM was totally NK‐inactive also after IFN activation. Among healthy adult donors, autologous BM was lysed as efficiently as allogeneic BM, and when different NK cell donors were used, the same classification of these as NK high or low reactive cells was seen regardless of the source of BM targets. IFN could augment lysis in autologous as well as in allogeneic effector BM target combinations. IFN could also protect adult BM cells from NK lysis, but no protection was seen with fetal BM cells. The highest NK activity against BM targets was seen among nylon‐nonadherent, E rosette‐negative PBL and, therefore, the effector cell seemed to be of the same nature as that active against continuous cell lines.These results show that in the human NK system, NK cells can lyse normal BM cells and thymocytes. The higher sensitivity expressed by fetal BM cells and thymocytes as compared to adult cells may suggest an increased frequency of a particular primitive NK‐sensitive cell type in these tissues, a finding which is in line with results seen in th

ISSN:0014-2980    

DOI:10.1002/eji.1830110103

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractThe capacity of (C57BL/6 × DBA/2)F1mice to produce anti‐(4‐hydroxy‐3‐iodo‐5‐nitrophenyl)acetyl (NIP) IgE antibodies, as a result of immunization with 1 μg of NIP2‐ovalbumin (OA) in the presence of Al(OH)3, was specifically suppressed by treatment of mice, either before or after immunization, with tolerogenic conjugates consisting of the hapten coupled to poly‐N‐vinylpyrrolidone (PVP) with an average mol. wt. of 10000. The suppression was hapten‐specific, since it did not affect the immune response of the host to OA. The unresponsive state of the spleen cells of mice which had been tolerized with respect to NIP was maintained even after cell transfer into X‐irradiated, syngeneic recipients and was shown to be due to hapten‐specific suppressor cells. However, the splenic B cells of these mice did not possess any suppressive activity. The generation of an effective number of suppressor cells required between 1 and 2 days following the administration of the tolerogen. The suppressive effects observed on adoptive transfer could not be attributed to the carryover of the tolerogen, which might have been associated with the spleen cells of the donor mice, since the transfer of B cells of tolerized mice or of mice which had received 2 mg of the tolerogen 2‐24 h before cell transfer did not abrogate the capacity of spleen cells of immune mice to mount an anti‐NIP response. Hence, it may be concluded that NIP2‐PVP induced, in addition to a probable receptor blockade of B cells, a central inhibitory mechanism which led to the development of an effective number of suppressor cells within 1 to 2 days a

ISSN:0014-2980    

DOI:10.1002/eji.1830110104

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractThe monoclonal antibodies, anti‐T1 and anti‐T3, both react with all human peripheral thymus‐derived lymphocytes and with 10% of thymocytes; each, however, recognizes different cell surface structures. It was determined that the target antigen of anti‐T1 is a 69000 molecular weight cell surface glycoprotein and that the T3 antigen is a 19000 mol. wt. glyco

ISSN:0014-2980    

DOI:10.1002/eji.1830110105

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractThe recently detected T axis differentiation antigen, which was provisionally designated as T‐lymphocyte‐macrophage‐associated antigen (TLMA) could now be identified as the neutral glycosphingolipid GgOse4Cer (asialo‐GM1).TLMA is not only expressed on lymphocytes on the T lineage and on macrophages of the rat, but also on eosinophilic cells. On erythrocytes, the determinants are only detectable after neuraminidase treatment.Within the cell surface of thymic lymphocytes, the antigen determinant is partly masked by sialic acid residues. Presently, it cannot be decided whether the masking effect is brought about by sialic acid residues of adjacent glycolipid molecules or whether a nonreactive sialylated precursor molecule

ISSN:0014-2980    

DOI:10.1002/eji.1830110106

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractMurine anti‐idiotypic serum against C3H.SW anti‐poly(LTyr, LGlu)‐poly(DLAla)–poly(LLys) [(T, G)‐A–L] antibodies was elicited in C57BL/6 mice. The effect of the anti‐idiotypes on the proliferation of primed lymph node cells was investigated. The anti‐idiotypic serum stimulated the proliferative response of the (T, G)‐A–L‐specific lymph node cells as well as of nylon wool‐enriched T cells. In the presence of suboptimal doses of (T, G)‐A–L, the addition of the anti‐idiotypes enhanced the proliferation to the levels obtained with the optimal dose of (T, G)‐A–L itself. These results suggest the existence of shared idiotypic determinants between antibodies and the (T, G)

ISSN:0014-2980    

DOI:10.1002/eji.1830110107

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractThe ontogeny of the B lymphocyte population to be capable of producing a high‐affinity plaque‐forming cell (PFC) response to a haptenic determinant was studied by cell‐transfer techniques in five different mouse strains. Lethally irradiated adult mice were reconstituted with liver from fetal or neonatal donors or spleen from young donors as a source of B lymphocytes. In addition, all animals received 1 × 108adult thymus cells. Recipients were immunized with dinitrophenylated bovine gamma‐globulin (DNP‐BGG) one day after cell transfer. The heterogeneity of affinity of their anti‐DNP PFC response was assayed by hapten inhibition of plaque formation. It was established by use of congenic mice differing at the Igh locus that only donor cells responded under these conditions. By use of this transfer system, it was shown that the B cell populations of A/HeJ, AKR and C57L/J mice aquire the capacity to produce a high‐affinity PFC response between day 15 and 18 of fetal life. The B cell populations of LAF1and DBA/1 J mice mature between 7 and 10 days after birth. The B cell population of C57BL/6 J mice matures between 21 and 28 days of age. In LAF1and AKR mice, the B cell population acquires the capacity to generate a high‐affinity indirect PFC response at approximately the same time as the capacity to generate a high‐affinity direct PFC response. The time of maturation of the capacity to generate indirect PFC was found to differ in different strains of mice. Maturation of the capacity to produce indirect PFC appears to represent a dinstinctly different differentiation event from the acquisition of the capacity to produce a high‐affinity PFC response. The data suggest that the maturation of the B cell population to be capable of producing high‐affinity PFC is a precisely regulated differentiation event, the timing of which is strain‐related. Preliminary data suggest that this regulation is not linked to either the H‐2 haplot

ISSN:0014-2980    

DOI:10.1002/eji.1830110108

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractA strong cell‐mediated immune response against Friend, Moloney, Rauscher virus‐induced (FMR) cell surface antigens has been demonstrated previously in mice which reject oncornavirus‐induced tumors. In order to identify an eventual suppressor mechanism in animals with progressively growing tumors, experiments were initiated in C 57 BL/6 mice bearing either a murine sarcoma virus (MSV) tumor or Moloney virus‐induced lymphoma (MBL2). Progressive tumor growth was induced (a) in viremic animals first infected with Moloney murine leukemia virus (M.MuLV), then inoculated as adult with MSV; (b) in nonviremic animals injected with MBL2 lymphoma cells. In the absence of tumor cells, viremia induces specific tolerance for which there is no evidence for suppressor cells. In tumor‐bearing mice, specific suppressor T cells are detected which are able to inhibit the generation of anti‐FMR cytolytic T lymphocytesin vitroand enhance the tumor growthin vivo.In addition to the specific suppressor T cells, a nonspecific suppressive activity mediated by metastatic T lymphoma cells is demonstrated in the spleens of lymphoma‐bearing animals. The respective role of the virus and tumor cells in the induction of tolerance to M.MuLV‐induced antigens, and their relationship to other components of the specific cell‐mediated immune resp

ISSN:0014-2980    

DOI:10.1002/eji.1830110109

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractPrevious reports have demonstrated that adult C 57 BL/6 mice infected with murine sarcoma virus (MSV) develop a strong cell‐mediated immune response against Friend, Moloney, Rauscher virus‐induced type‐specific (FMR) antigens and reject their tumors. To demonstrate a possible role for auto‐anti‐MSV T blasts, syngeneic C 57 BL/6 mice were immunized with highly enriched anti‐FMR cytolytic T cells. One of 3 pools of these autoimmune T cells prepared from 12 surviving immunized mice (a) inhibited specifically thein vitroanti‐MSV cytolysis generation and (b) enhanced drastically the MSV tumor growthin vivo.The possibility for such an immunization procedure to induce anti‐idiotype T cells, the repeatability of this effect and the relationship of the suppressor cells with antigen‐specific suppressor cells and other components of the anti‐MSV immune res

ISSN:0014-2980    

DOI:10.1002/eji.1830110110

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractThe role of the oligosaccharide portions of cell surface glycoproteins in the susceptibility of virus‐infected cells to H‐2‐restricted cytolysis was investigated by using the antibiotic tunicamycin (TM). TM inhibits the addition of sugars to the polypeptides of glycoproteins. TM treatment of P 815 cells before and during infection with vesicular stomatitis virus (VSV) inhibited glycosylation of proteins and reduced by about 50% the lysis of infected P 815 cells by VSV‐immune, H‐2‐identical killer cells. In contrast, TM treatment had a modest inhibitory effect on cytolysis of P 815 cells by alloimmune effector cells. TM treatment did not inhibit the surface expression of either H‐2 or VSV glycoprotein. Thus, glycosylation of H‐2 and/or viral glycoprotein is a prerequisite for the lysis of infected cells by H‐2‐identical, VSV‐i

ISSN:0014-2980    

DOI:10.1002/eji.1830110111

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY