ISSN:0014-2980    

DOI:10.1002/eji.1830120102

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

ISSN:0014-2980    

DOI:10.1002/eji.1830120103

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractThe ontogeny of acquisition of complement receptors (CR) on splenic B lymphocytes from mice of varying ages was examined. Reagents used for the identification of CR included antibody‐ and complement‐coated erythrocytes (EAC), bacteria (BAC) and bacteria treated with complement alone (BC). When whole mouse serum was used as the source of Complement both EAC and BAC failed to bind to human erythrocytes (C3b receptor‐negative). showed binding to Raji and Daudi cells (C3d receptor) and formed rosettes with human neutrophils (C3bi receptor). Therefore these reagents bore C3d and C3bi, but not intact C3b. Although EAC and BAC detected nearly equal percentages of CR lymphocytes in adult mice, BAC bound to a higher percentage of neonatal lymphocytes (8‐1296) than did EAC (1‐395). The ability of BAC to detect EAC‐negative lymphocytes among neonatal spleen lymphocytes appeared to be due to the increased sensitivity of this bacterial reagent for binding to CR. Further evidence of this enhanced sensitivity was that BAC were less inhibitable than EAC by soluble antigen‐antibody‐compl

ISSN:0014-2980    

DOI:10.1002/eji.1830120104

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractMouse spleen cells were fractionated and tested for their ability to stimulate a primary mixed leukocyte response (MLR). Several nonlymphoid populations were stimulatory, purified dendritic cells and 18‐h adherent macrophages being the most effective. B and T lymphocytes and peritoneal washout cells were almost ineffective by comparison. This hierarchy of stimulator potential was observed whether the genetic difference between stimulator and responder was whole H‐2 or H‐2 subregion (K. D. I. I‐J). Allostimulatory populations contained a high proportion of cells expressing I region determinants. The functional importance of these antigens was confirmed using specific anti‐I region antisera. Thus, it appears that stimulator cells in the MLR are heterogeneous, Ia‐bearing, and of “accessory” rather tha

ISSN:0014-2980    

DOI:10.1002/eji.1830120105

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractEvidence is presented that T helper cells change their Lyt‐1,2 phenotype, from Lyt‐1+2−to Lyt‐1−2+, during a secondary response. In a secondary adoptive transfer the Lyt phenotype of carrier‐primed T cells was established before and after secondary challenge using monoclonal antibodies and both positive and negative selection in the fluorescence‐activated cell sorter. In a carrier‐primed donor, the T helper cells were Lyt‐1+2−whereas 3 days after boosting the majority were Lyt‐2+, and resistant to treatment with anti‐Lyt‐1 and complement. Parking experiments in lethally irradiated recipients confirmed that Lyt‐2+T helper cells were generated from Lyt‐2−precursors. In a primary response, both Lyt‐2−and Lyt‐2+T helper cells were present 4 to 20 days after immunization. Therefore T helper cells do not exhibit invariant e

ISSN:0014-2980    

DOI:10.1002/eji.1830120106

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractAllotypic restriction of idiotype suppression has been shown in several idiotypic systems. It has also been demonstrated that naive BALB/c mice contain naturally occurring suppressor T cells capable of inhibiting the M‐460 idiotype expressed on BALB/c B cells, and that T cells from allotype‐congenic mice have no effect on this expression. Since we have previously shown that upon polyclonal activation B cells of all mice tested are capable of secreting M‐460‐positive anti‐2,4,6‐trinitrophenyl (TNP) antibodies, we postulated that idiotype suppressor cells may recognize idiotypes in conjugation with products of the Igh loci. In the present study we tested this hypothesis. The data clearly showed that regardless of their allotypic haplotype all strains of mice do possess suppressor cells capable of recognizing the M‐460 idiotype. However, mixing experiments indicated that the functional potential of these suppressor cells can only be expressed when T and B lymphocytes come from allotype‐matched animals. Efficient suppression in those cultures containing allotype‐matched but incompatible B and T cells at the I‐J region of the H‐2 locus could be detected.Finally, using allotypic recombinant strains of mice, we found evidence that the restrictive elements in idiotypic suppression are the product of genes mapping in the Igh‐C locus,

ISSN:0014-2980    

DOI:10.1002/eji.1830120107

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractB cells could be stimulated to proliferate by mitomycin C‐treated normal T cells that were activated by rabbit anti‐mouse brain antiserum (RaMBr). The B cell proliferation peaked 48 h after the initiation of the cultures. The T cell subset involved was Lyt‐1+, 2−. The B and T cells participating in the interaction did not need to be matched at the H‐2 locus. A long‐term T cell line (C.1.a), which was genetically restricted by the H‐2 complex in its antigen (ovalbumin)‐specific interaction with B cells, could also be induced by RaMBr to stimulate B cell proliferation. However, the RaMBr‐mediated interaction of C.1.a with B cells was genetically unrestricted. This suggests that T cell activation by RaMBr can bypass the requirement for the recognition of I region antigens, which is a feature of antigen‐specific interactions between T and B cells. In fact, RaMBr antibody appeared to function by bringing T cells into contact with B cells by the interaction of the B cell surface membrane Fc receptors with the Fc portion of RaMBr antibody bound to the T cell surface. Brain‐associated antigen(s) appeared to play a unique role in T‐B cell interactions because antibodies against other T cell surface antigens were inactive,e.g.those which remained in rabbit anti‐mouse thymocyte antiserum at a high titer after absorption with mouse brain tissue. Furthermore, the brain‐associated T cell antigen involved in the cellular interaction was differentiated from the Thy‐1 antigen by the failure of xeno‐anti‐Thy‐1.1 antisera to induce the interaction of B6.PL.Thy‐12T cells with B cells, and the presence of activity in rat anti‐AKR mouse brain serum which did not contain antibodies against Thy‐1.1 or Thy‐1.2 determinants.The data appear to support the hypothesis that the brain‐associated T cell antigen, as defined serologically in these studies, may represent a molecule which plays a role in the immunological

ISSN:0014-2980    

DOI:10.1002/eji.1830120108

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractIa.W39 is a B cell differentiation antigen whose membrane expression is controlled by the xid gene. In this report we show that, analogous to its B cell expression, Ia.W39 is also present on a subset of Ia+macrophages, indicating heterogeneity within that cell population.The Ir gene(s) for the antigenic determinants on the A chain loop of beef insulin maps to the I‐Absubregion of the H‐2 complex and, as we have previously reported, is associated with the private specificity Ia.W39. Depletion of Ia.W39+macrophages eliminates their capacity to present beef insulin to immune T cells, whereas the presentation of the multideterminant antigen trinitrophenylated ovalbumin is reduced less than 50%. Furthermore, we found that H‐2bmice lacking Ia.W39+cells are unable to make a secondaryin vivoIgG plaque‐forming cell (PFC) response to beef insulin, while the primary IgG PFC response is not dependent on Ia.W39. No shift in the kinetics of the response, nor development of suppressor T cells could be detected in Ia.W39−mice, which would explain their apparent nonresponsiveness to beef insulin after boosting with this antigen. These results, therefore, may reflect a difference in the Ir gene control acting at the primaryvs. secondary respo

ISSN:0014-2980    

DOI:10.1002/eji.1830120109

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractMonoclonal anti‐Qat‐4 and anti‐Qat‐5 antibodies, which define antigens expressed on peripheral T cell subsets, have been used to study the phenotypes of alloreactive and H‐2‐restricted cytotoxic effector cells and their precursors. Depletion of Qat‐4+or Qat‐5+cells from the T cell pool prior to their sensitization in bulk cultures prevented the development of alloreactive and H‐2‐restricted cytotoxic activities in the selected populations. No reconstitution of cytolytic activities to normal levels was obtained when mixtures of Qat‐4+and Oat‐5+cells were sensitized in bulk cultures to H‐2 or non‐H‐2 antigens. Sensitization of limiting numbers of Qat‐4−or Qat‐5−lymphocytes under optimal conditions for help (interleukin 2), with the appropriate antigens (H‐2, H‐Y) did not result in the generation of cytotoxic T cells, indicating that the majority of all cytotoxic T lymphocyte (CTL) precursors are Qat‐4+, Qat‐5+.When CTL effector populations were treated with the antisera and complement (C) at their maximum CTL activity, it was found that H‐2‐restricted CTL were totally eliminated by anti‐Qat‐4 and considerably reduced by anti‐Qat‐5 antisera and C. In contrast, alloreactive CTL effector cells were insensitive to anti‐Qat‐4 and to anti‐Qat‐5 plus c. Although alloreactive CTL effector populations regained some Qat‐4 antigens during furtherin vitroculture, it was shown that H‐2‐restricted CTL were at all times more sensitive to anti‐Qat‐4 than were alloreactive CTL. The findings suggest that during maturation of alloreactive and H‐2‐restricted CTL from their precursors, both alloantigens undergo differential quantitative variations in their expression t

ISSN:0014-2980    

DOI:10.1002/eji.1830120110

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY

摘要:

AbstractPrimary (1o) and secondary (2o) syngeneic mixed lymphocyte culture (SMLC) reactions in autochthonous murine thymus cell cultures (peanut agglutinin‐unreactive) or syngeneic mixed thymus‐spleen cell cultures were studied. The 1othymic SMLC responder cells were Thy‐1+, Lyt‐1+, Lyt‐2−. The 1othymic syngeneic mixed lymphocyte reaction (SMLR) could be inhibited with monoclonal antibodies directed against determinants Ia.m1 and Ia.m6, in the absence of complement.Positive selection of thymic SMLC cells from 1ocultures was achieved by enriching reactive lymphoblasts on discontinuous Percoll density gradients. The 2omemory populations were obtained by allowing the selected lymphoblasts to revert back to small resting 2ocells in the absence of SMLC stimulation. The thymic 2oSMLC populations were highly enriched in their activity and were almost exclusively specific for self stimulator cells.The 2othymic SMLC cells were found to be Thy‐1+, membrane immunoglobulin‐negative, H‐2K/D+, I‐A−, I‐E−and I‐J−. They were hardly lysed by anti‐Lyt‐1 antibodies and not at all lysed by monoclonal antibodies detecting Qat‐4 and 5, and Lyt‐2 antigens, in the presence of complement. Stimulator cells were found in fast‐sedimenting or low‐density fractions of thymus. They expressed H‐2K/D, I‐A‐, and I‐E antigens but lacked membrane immunoglobulin and Thy‐1. Not all I region determinants seemed to be of equal importance in the 2othymic SMLC: I‐A subregion compatibility between responding and stimulating cells was a minimal requirement for the induction of 2othymic SMLC responses. Although several relevant anti I‐region antibodies reacted with the stimulator cells in the presence of C, these and other anti‐Ia antibodies varied considerably in their capacity to block 1oand 2oautochthonous thymic SMLR in the absence of complement.Our finding that the thymus contains the responder as well as the stimulator cells capable of interacting in an autochthonous proliferative reaction will be discussed with regard to its implicat

ISSN:0014-2980    

DOI:10.1002/eji.1830120111

出版商:WILEY‐VCH Verlag GmbH    

年代:1982

数据来源: WILEY