摘要:

JANUARY 1932 Vol. LVII. No. 670 THE ANALYST PROCEEDINGS OF THE SOCIETY OF PUBLIC ANALYSTS AND OTHER ANALYTICAL CHEMISTS AN Ordinary Meeting of the Society was held at the Chemical Society’s Rooms, Burlington House on Wednesday December 2nd the President Dr. J. T. Dunn, being in the chair, Certificates were read for the first time in favour of:-Albert Green M.C., M.Sc. Ph.D. F.I.C. John Farrar Hardwick B.Sc. A.I.C. Ernest Stephen Hawkins, B.Sc. A.R.C.S. F.I.C. Joseph Robert Johnson F.I.C. M.Inst.M.M. Arthur Pillans Laurie M.A. D.Sc. F.R.S.E. and John Morgan Tucker B.Sc. A.I.C. Certificates were read for the second time in favour of :-Thomas Whittaker Lovett and William Charles Alfred Wise B.Sc. The following were elected Members of the Society :-Charles Hubert Francis Fuller B.Sc.F.I.C. Ganesh Chandra Moitra BSc. Eric Charles Wood B.Sc., A.R.C.S. and Robinson Pearson Wood. The following papers were read and discussed:-“ A Micro-method for the Determination of Uronic Anhydride Groups in Pectic Substances,” by H. W. Buston Ph.D. D.I.C.; “The Composition of Linseed Oil,” by N. E. Cocchinaras, Ph.D.; “Oil from Malayan Alezwites Mofttmza and the Properties of Hong Kong Oil,” by T. Hedley Barry; and “The Calcium Fluoride Method for the Determina-tion of Fluoride with Special Reference to the Analysis of Nickel Plating Solu-tions,” by S. G. Clarke Ph.D. A.I.C. and W. N. Bradshaw B.Sc. NORTH OF ENGLAND SECTION A MEEriNG of the Section was held in Leeds on December 5th 1931. There were 28 present and the Chairman (Mr. C. J. H. Stock) presided. Dr. Dunn President of the Society read a paper on “The Public Analyst axid his Professional Relationships,” in which he stated clearly the principles which he suggested a Public Analyst should maintain drawing illustrations from his own personal experience. Many members took part in the discussion which followed. A resolution of sympathy was passed with the relatives of the late Mr. W. Foulkes Lowe.

ISSN:0003-2654    

DOI:10.1039/AN9325700001

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

2 MABY THE IDENTIFICATION OF WOOD AND WOOD CHARCOAL FRAGMENTS The Identification of Wood and Wood Charcoal Fragments BY J. CECIL MABY B.Sc. A.R.C.S. (Rend at the Meeting October 7th 1931) ~NTROUUCTION .-It is now many years since forest products resmrcli as distinct from the much older science of sylviculture first began to receive expert attention and certain common woods to be subjected to anatomical examination;* while more recently the physical and chemical Properties of wood have been made the subject of special investigations both in America and in Europe with an increasing recognition of the enormous value of wood substance considered either as convertible timber or as a complex of chemical compounds. The physics and chemistry of wood however I shall not discuss in the present paper my object being briefly to outline the value and importance of a purely anatomical knowledge of the many thousands of commercial woods which have been and are still used throughout the world for constructional purposes cabinet work and the manufacture of innumerable articles of everyday utility.As is well known to microscopists each kind of wood possesses its own specific structure and physical properties the knowledge of which from originally being a matter of purely botanical significance was quickly seen to be also of considerable economic and forensic importance. Thus it was thought possible by determining the average range of structure and percentage amounts of the various elements in a number of samples belonging to a given commercial species to deduce therefrom certain standards of wood structure for the species as also in relation to particular purposes and industries.Such standards are still in the process of being workedf out with an ultimate intention first of enabling the selection of the best type and quality of wood for a given purpose; and second so that sylviculturalists may know more certainly what types of timber are required of them and how to grow such timber most economically. Moreover it has been found that detailed knowledge of the specific structure of commercial woods renders possible the identification of quite minute samples of -unknown timber. That such identifications may frequently be of value in commerce in archaeology and for forensic purposes will be easily appreciated.Indeed in the few years since the inauguration of the two principal institutes in this country $ many hundreds of crucial identifications have already been carried out for firms and individuals. For instance a company may purchase a large consignment of timber purporting to be mahogany or American white * Vide for example the work of Robert Hartig in Germany up011 beech and oak towards t Such investigations are being iiiaclc in this country upon coinmon ash elm and oak in the $ The Forest Products Research Laboratory (L).S,I.R.) Princes Kisborough Bucks. and the end of the last century. last of which I have been engaged. the Imperial Forestry Institute Oxford MABY THE IDENTIFICATION OF WOOD AND WOOD CHARCOAL FRAGMENTS 3 oak say; whereas the consignments may be found by the technologist to be no better than a cheaper and poorer substitute for mahogany (a name often used extremely loosely since the world’s resources of true mahogany have been practically depleted) and in the second place American swamp oak-an almost useless timber which for some undetermined physico-chemical reason is liable to extreme “ collapse ”* on seasoning.Again pieces of wood and wood charcoal from fallen burnt and buried edifices, the handles of ancient implements portions of inhumated and fossilised trees etc., are constantly requiring identification in order to prove some legal point to help the antiquarian to solve his historical problems or to extend our botanical know-ledge of the past flora of a country or district. Not a year passes but many interesting archaeological finds are made in this country alone of charcoals and wood fragments portions of charred beams and posts the remains of stockades piles from lake dwellings spear hafts implement handles charcoals from ancient hearths and furnaces-one and all can be identified.I have personally been fortunate in receiving many such remains for examination during the past few years and can say at once that the instances are few in which interesting information is not thereby acquired. For example it has been possible to show that red oakwood-presumably Qwrczcs cerris-was used in an Elizabethan glass furnace at Hambledon Surrey although the tree was not previously supposed to have been introduced until about 1735; while the existence of beech in south-west England long before Roman times has been corroborated by several discoveries running back to early Neolithic times.In other cases it has been possible from the species and apparent sizes of wood employed to confirm the hypotheses of the excavators as to the nature of the structures in question. Other interesting facts also appear such as the use of a seemingly Indian wood in a Roman spear-haft found near St. Albans and the occurrence of now exotic timbers in certain ancient Egyptian structures. THE PREPARATION OF MATERIAL-The mode of preparation of materid for microscopic examination will depend chiefly upon its physical condition this being determined by its past history. If it is thought necessary to section a specimen, either for purposes of detailed investigation or for photomicrography and record, the preliminary treatment may then be relatively long and delicate.Often, however-especially with the better-known woods-it is unnecessary for an experienced wood technologist to cut sections at all a superficial view obtainable by means of a good hand-lens or low-power binocular microscope frequently being sufficient to permit of identification. In fact I have generally found that wood charcoals may best be broken across by hand to show a clean clear cross-section of the grain (the most useful view). Practically as much is thus seen superficially as in a thin microscopic section made from embedded material for the intense blackness of the tissues often renders the more minute structures (e.g. pitting of the cell walls) invisible by transmitted light.* “Collapse” is a remarkable form of distortion and exaggerated shrinkage occurring in certain timbers during seasoning. The phenomenon which is not to be confused with warping or irregular shrinkage proper is found to result from an actual collapse of the cell walls-worse in some parts than in others 4 MABY THE IDENTIFICATION OF WOOD AND WOOD CHARCOAL FRAGMENTS An average charcoal sample-even after three or four thousand years under ground-normally preserves every main detail of the wood structure in wonderful perfection whereas uncharred and sodden wood is frequently found to have suffered considerable internal decay and dissolution often accompanied by extreme compression and “ collapse.” Hence unless the specimens have become partly vitrified or “ coaly ” (reason unknown) as does sometimes happen, charcoals may usually be said to present better material for identification than excavated wood.Rarely however charcoal fragments are dug up in so damp and crumbly a condition that no kind of treatment may avail to preserve their form for microscopic examination. Such specimens are not brittle enough to be broken across as described above and they will not always stand slow oven-drying or treatment with alcohol previous to embedding. The only other alternative seems to be very slow drying in a desiccator employing hygroscopic salts. But even then the specimens might break up when dropped into the solvent spirit for embedding. This I have not yet attempted. Very rotten wood samples as well as charcoals may also be examined super-ficially by breaking across the grain-never by cutting even with the sharpest razor; an alternative method is that of careful impregnation with wax or celloidin as described below.In making superficial examinations whether of wood or charcoal whereof sufficient detail can thus be observed a hand lens though useful is uncertain and trying to the eyes. A good low-power binocular microscope magnifying X 20-30 lin. and having a large field of view (e.g. not less than 25 sq. mm.) is, therefore almost indispensable. The only other and critical factor as I need scarcely mention is the incidence and intensity of illumination. Material should, however be classified as normally requiring one of four kinds of preliminary preparation according to circumstances.Wood that can be sectioned in a microtome without preliminary treatment of any kind must have been wet-ratted in an unusual manner and is but rarely met with. Even so the preliminary in-filtration of some binding substance is highly desirable. These four treatments of which the first two have already been considered, So far I have spoken only of superficial examination. are as follows:-(1) Brittle specimens of coarse texture or familiar structure. (2) Tough wood specimens of rela-tively coarse texture or familiar structure. (3) Normal tough specimens of wood, whether ancient or modern of fine texture or unfamiliar struc-ture. (4) Specimens of fine texture un-familiar charcoals and rotten or crumbling specimens of wood or charcoal.Break sharply across the grain in the hand cleave longitudinally (not cut), and examine superficially. Cut trhsversely with a sharp knife, cleave longitudinally (not cut) and examine superficially. First soften by boiling in water or by treatment with hydrofluoric acid or both followed by pickling in glycerin and alcohol. Section on a micro-tome. Embed in either paraffin wax or celloidin according to relative hard-ness and section with a microtome for detailed examination MABY THE IDENTIFICATION OF WOOD AND WOOD CHARCOAL FRAGMENTS 5 With regard to the third group ordinary wood samples may be said to be either very hard hard medium or soft; the first three types are capable of blunting a good steel knife too quickly to enable large thin sections to be cut for microscopic purposes until the siliceous skeleton which pervades the entire cellular meshwork has first been dissolved away by means of hydrofluoric acid.Common oak for instance may be classed as “medium to hard,” whilst most tropical woods are “very hard.” Coniferous timbers on the other hand are usually soft enough to cut well after 5 to 10 hours’ boiling in water and some storage in a mixture (50:50) of methyl alcohol and glycerin lasting say from a week to twelve months. But the latter solution being used largely for storage purposes may when time is short be omitted. Moreover as is well known, glycerin has a softening action upon many organic tissues and alcohol eventually spoils staining properties; so that storage in this solution can be over-prolonged.It is usual therefore to give the hard woods a treatment of one to four weeks in hydrofluoric acid diluted down to about 50 per cent. with water in accordance with the relative hardness of the wood and to keep the material in hard rubber bottles; this step can be omitted with soft woods. The acid of course must be thoroughly washed out before transferring the material to 95 per cent. alcohol, and finally to the alcohol-glycerin storage mixture. Some woods possess sufficient natural colour not to require staining but wood sections may be suitably stained with iron and haematoxylin followed by safranine,* and finally mounted after clearing with xylol in Canada balsam. When less permanent mounts are required glycerin jelly mixed with a suitable stain such as methyl blue may be used as a mounting medium; considerable expense and time are thereby saved.The degree of softening desirable is ascertained by experience; but given a really sharp knife and a good sledge microtome (Reichert’s models and Jung knives give excellent results) a wood block should cut like rather hard cheese at a thickness of 10 to 20p. It is usual to employ sample blocks of about 10 by 10 by 15 mm. in size and t o cut radial longitudinal tangential longitudinal and transverse sections the last being the most useful for general diagnostic purposes. Finally in respect of the fourth class of material it used I believe to be thought that charcoals were too hard and brittle to be sectioned in the usual way, but I found that a solution of commercial cellulose nitrate known as “Celloidin,” made as effective an embedding matrix for charcoals as it does for chitinous insects.The embedding is carried out in the usual way on these lines (1) Removal of all air from the tissues with a filter pump while the samples are in 50 per cent. alcohol. (2) Desiccation by means of graded alcohols ending with a mixture of absolute alcohol and methyl ether (50:50). (3) Transference of the samples to air-tight screw-topped bottles which are kept in an oven at 60” C. and contain celloidin dissolved in ether and alcohol; the concentration of the solution is increased, day by day from 2 to 16 per cent. (4) Removal of the samples from the 16 per * A solution of a mixture of the alcohol-soluble and water-soluble safranines in equal parts, Dilute is satisfactory.solutions are best. This combined with haematoxylin is excellent for photomicrography 6 MABY THE IDENTIFICATION OF WOOD AND WOOD CHARCOAL FRAGMENTS cent. celloidin to be dropped into a mixture of ether and chloroform (50:50) in which they remain until hardened and ready to section. After sectioning it is unwise to risk dissolving away the “Celloidin,” which may be mounted infiltrated as it is with the section either in glycerin jelly or in balsam. In this connection I have found that good clear mounts may be made by using Gurr’s mounting medium which appears slowly to dissolve away the celloidin so clearing the section. In microtoming 95 per cent. alcohol may be used as lubricant as in cutting ordinary wood blocks; nor have I found the use of the coloured commercial product to affect the results.A much quicker and cheaper though less perfect and reliable technique than the latter is first to dry the charcoals and then to dip them in a dilute solution of celloidin (or of cellulose acetate in acetone) re-painting the cut surface after each section. But when used in this way the solution tends to contract on drying and it does not bind the tissues so thoroughly. For soft decayed wood samples the cheaper and easier paraffin-wax method is suitable. But the technique is too well known for repetition here; xylol is used as paraffin solvent with warming in an oven during impregnation as before. In addition to these methods impregnation with a synthetic resin was kindly tried for me some while ago at the Building Research Station (D.S.I.R.) where a resin of the following composition (in molecular weight proportions) has been found suitable for the preparation of thin sections of friable and weathered materials:-Phenol 3; formaldehyde 6; meta-cresol 3 parts.In this way the very hardest materials can be successfully embedded and subsequently cut by a steel and diamond wheel as in petrology; but as’ the method is unnecessarily laborious and scarcely so successful as celloidin embedding it need not be given in detail. of which the wood of one genus may be distinguished from that of another or-less commonly and surely-that of one species from another are as follows:-(i) The relative size distribution frequency and configuration of the condactafig vessels (commonly known as “pores”) as seen in transverse section.(ii) The relative size distribution and frequency of the medullary rays as seen in transverse and tangential longitudinal sections. (iii) The type grouping and distribution of the woodcfibyes,* as seen in transverse section. (iv) The amount and arrangement of the vertical storage parenchyma as seen in transverse and radial longitudinal sections. (v) The presence or absence and disposition of vertical and horizontal resin dacts as seen in transverse and tangential longitudinal sections. These features are usually observable with a low-power microscope or hand-lens; but in very closely similar woods-many of the conifers for instance-it is * The fibres are long elements often occurring in bundles (e.g.in oak) which give mechanical strength to the wood. Fibres do not occur in conifers (“ soft woods ”) being replaced by thick-walled tracheids. EXAMINATION AND IDENTIFICATION.-The chief diagnostic features by mean PLATE I. 1 Scotch pine (Yinus sylvestris) 4 Field maple (Acer campestre) STRUCTURE OF WOODS 2 Sweet chestnut (Custan~u vesca) 6 Hazel (Covylzts auellana) 3 Common elm (Ulmzcs cumpestris) 6 Black poplar (Populzcs n i p a ) All are transverse sections x 10 linear as viewed under surface illumination MABY THE IDENTIFICATION OF WOOD AND WOOD CHARCOAL FRAGMENTS 7 often necessary to make use of yet more minute structures such as are only visible in a good section and at high magnification; as for example pits in the cell was, the height width and frequency of the small rays and the form of the cells com-posing the latter.But in not a few instances two distinct species may resemble one another so closely that even these minute distinctions fail. Thus from knowledge of the wood structure of a single small sample it is often unsafe to deduce more than the broad genas. For instance many species of elm are hard to differentiate so are certain oaks; whilst the poplars and willows not only cannot safely be dis-tinguished specifically but poplar and willow woods may also be generically in-distinguishable in surface view. And so on with other groups of closely related genera. The two related species evergreen oak (Q. iZex) and white oak (Q. &a), however would not readily be confused.Reference to Plate I will illustrate the following diagnostic features :-Coniferous woods (“ softwoods ”) are immediately distinguishable as a group from the Angiospermous flowering trees (“ hardwoods ”) by the fact that they never possess vessels (“pores”) though they may or may not contain resin ducts-a group and generic distinction. Again in a conifer the more recently-formed wood of a growth-ring may or may not be sharply contrasted with the rest of the ring in the colour or thickness of its cell walls or both. In conifers the rays are always fine (Fig. 1); in the Angiosperms they are sometimes fine and numerous (Figs. 2 and 6) sometimes thicker and less numerous (Figs. 3 and 4); and occasionally very large rays visible to the naked eye alternate with small ones (Fig.5). It will also be seen from Plate I that the “ pores ” in hardwoods are of variable size and arrangement ranging from “diffuse porosity” (Fig. 4) by way of trans-itional forms (Figs. 6 5 and 2) to marked “ring porosity” (Fig. 3)-vix. a sharp transition at the beginning of the ring from large to small pores. But one should add that owing to the enormous number of woods of economic utility it is a great help to the wood technologist in identifying unknown samples to be told if possible, the country of origin. Yet even that is of little avail when the region happens to be India S. America or Africa for example where the number of indigenous species runs into thousands and whence others are still being imported.In all such cases therefore it is usual to construct both macroscopic and microscopic “ keys ” based upon the various anatomical characteristics mentioned above, fibs features such as colour hardness taste smell and density. These “keys,” when comprehensively planned facilitate the rapid identification of unknown wood samples. But individual variation and aberration from type are serious obstacles; a large collection of authentic wood samples and microscopic sections is therefore desirable in order that the wood technologist may also check his conclusions. (See Fig. 1.) More detailed features need not be enumerated here. NOTES ON THE ILLusTRATIoNs.-PZate I represents surface views of small fragments of six common European woods as seen in transverse section magnified x 10 linear 8 MABY THE IDENTIFICATION OF WOOD AND WOOD CHARCOAL FRAGMENTS Plate I I shows still smaller areas from transverse sections of three English woods (two being slow grown with very narrow rings) as seen under the micro-scope by transmitted light magnified x 30 lin.But in this plate only the upper three figures depict normal wood; the lower three are Roman charcoals of the same woods submitted to me for identification in which similar only slightly shrunken, tissues and elements may be recognised. The charcoals were derived from a Roman smelting furnace dating back to about 300 A.D. discovered in Surrey and I am indebted to Mr. S. E. Winbolt for permission to use these photomicrographs as well as to the Director of the Forest Products Research Laboratory England and Prof.C. C. Forsaith of New York State College of Forestry under whose direction the work here described was initiated. 3 RAWLINSON ROAD, OXFORD. DISCUSSION Dr. CAMERON asked if there were any method for identifying charcoal when finely powdered. Mr. MABY replied that this was very difficult although occasionally in the case of an easily identifiable wood such as common oak he had been able to identify small splinters. ADDENDUM.-since the meeting my attention has been directed to a paper on “The Identification of Norit and other Wood Charcoals,” by H. G. Tanner (Ind. Ertg. Chem. 1925 17 191; ANALYST 1926 51 50). In an attempt to determine the nature of a highly activated decolorising charcoal called “Norit,” which was introduced about 1911 it was found that minute particles of cell wall substance (apparently from the walls of conducting vessels or tracheids) termed “ identifica-tion particles,” were always present.These particles examined under a high power of the microscope (say x500) were seen to be perforated by numerous minute pits the relative size frequency and pattern of which were seen to con-stitute a generic if not also a specific character. In this way it was concluded that “ Norit ” had been made from birch wood (Betzcla occidentalis 2). I should add that I have not yet had occasion to employ the method myself, and a considerable amount of systematic work would be required before it could be put into practice; but judging by what I have seen of cell-walled pitting in woods it should prove satisfactory. One possible objection which occurs to me is that the size form and arrange-ment of such pits undoubtedly vary to a considerable extent within a given species, or even from part to part of a single cell wall. Moreover a considerable amount of shrinkage (about 1/3?) takes place in wood when it is converted into charcoal. In incompletely charred specimens therefore the mean diameter of such pits might be greater than in specimens which have been treated for a longer period of time PLATE II. A Common oak (Qztevcw sp.) a STRUCTURE OF WOODS B Hazel (coYyz11s sp.) b C t Delow Roman charcoals (a b c) for comparison with the woods (A B C) shown above. ,411 are transverse sections x 30 linear as viewed by transmitted light

ISSN:0003-2654    

DOI:10.1039/AN9325700002

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic.This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm.up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation.Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity.CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C.of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view.Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned.Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium.Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms.of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view.Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium.Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C.of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN9325700009

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic.This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm.up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation.Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity.CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C.of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view.Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned.Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium.Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms.of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view.Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium.Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C.of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms.give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation.Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium.Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN9325700015

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic.This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm.up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation.Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity.CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C.of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN9325700022

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic.This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN9325700025

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic.This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN9325700027

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN932570029b

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN9325700030

出版商:RSC    

年代:1932

数据来源: RSC

摘要:

294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected.The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results.The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable.The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm.Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected.Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm.in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic.This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm.of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity. CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased.The best conditions for the detection of calcium were found to be as follows: Take 50 C.C. of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm.up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation.Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.294 EVERS: THE DETECTION OF SMALL QUANTITIES OF CALCIUM Adding 5 mgrms. of calcium. Added salts. Result. No added salt. Immediate pptn. Sodium chloride, 1 grm. Borax, 1 grm. Sodium potassium tartrate, 1 grm. Potassium citrate, 1 grm. Variations in the concentration of the reagents did not appreciably improve matters. It was found that even 0.25 grm. of potassium citrate in 60 C.C. of solution prevented the precipitation of 2 mgrms. of calcium. Further complications would be introduced if magnesium were also present in the salt as an impurity.CALCIUM OLEATE TEsT.-The formation of an opalescence on the addition of sodium oleate solution to a solution is an extremely delicate test for calcium. Under the best conditions 0.01 mgrm. of calcium in 50 C.C. of solution, or 0.00002 per cent., can just be detected. The test is also, of course, a test for magnesium, but is much less sensitive, 0-6 mgrm. in 50 C.C. of solution, or 0-0012 per cent., being the minimum quantity which can be detected. Further, within certain limits of concentration the pre- cipitation of magnesium is entirely suppressed in the presence of potassium citrate, whilst the sensitiveness of the calcium test is actually increased. The best conditions for the detection of calcium were found to be as follows: Take 50 C.C.of the solution containing calcium, which should be neutral or slightly alkaline. Dissolve in it 2 grms. of potassium citrate, and add 0-3 C.C. of a solution prepared by dissolving 10 grms. of oleic acid in 200 C.C. of 1 per cent. sodium hydroxide. A certain excess of alkali is desirable for the best results. The test is only satisfactory between certain limits of calcium concentration. With quantities exceeding 1 mgrm. in 60 C.C. the opalescence is actually reduced. Under the above conditions quantities of mag- nesium up to 15 mgrms. give no opalescence. Summarising the results, the oleate test is excellent for quantities of calcium varying from 0-01 mgrm. up to 1 mgrm. in the absence of more than 10 mgrms. of magnesium, and within these limits in the absence of other salts the opalescence appears proportional to the calcium present. Further experiments showed, however, that, in spite of its delicacy, the oleate test is not suitable for the purpose in view. Possibly, if the test could be carried out, using standards containing the same concentration of the same salt, it would be satisfactory, but this is hardly practicable. The addition of other salts, even in the absence of potassium citrate, caused the results to be erratic. This was partly due to their “salting out ” effect on the soap, which sometimes caused flocculation, but this was not the whole explanation. Almost immediate pptn. Slight ppt. after 30 minutes. Slight ppt. after 30 minutes. No ppt. This line of investigation was therefore abandoned. Mix and allow the mixture to stand. An excess of the reagent gives less opalescence.

ISSN:0003-2654    

DOI:10.1039/AN9325700031

出版商:RSC    

年代:1932

数据来源: RSC