摘要:

In this study we determined the influence of protein adsorption on substratum surface free energy, infrared absorption and cell spreading. We adsorbed bovine serum albumin, bovine fibronectin and foetal bovine serum during two hours onto three substrata: fluoroethylenepropylene copolymer, a low surface free energy material, polyvinylfluoride, a medium surface free energy material, and tissue culture polystyrene, a high surface free energy material. We determined the influence of protein adsorption by means of Fourier Transformed Infrared Spectroscopy (ATR‐mode), by measuring the changes in substratum surface free energy and by deterniining the equilibrium spreading of fibroblasts (approximately 2 h) from RPMI 1640 medium. Infrared spectra showed Amide I and Amide II bands, indicative for protein adsorption, located at different wavenumbers for different substrata. After protein adsorption, substratum surface free energies converged slowly, but a distinct influence of the bare substratum surface free energy remained. Ceil spreading slightly increased on protein‐coated substrata, but remained according to the pattern expected on basis of the surface free energy of the uncoated substrata, i.e. low on low‐energy substrata and high on high‐energy substrata. Only fibronectin caused a significant increase in cell spreading, possibly due to the specific interaction of adsorbed fibronectin with specific cellular receptors.

ISSN:0892-7014    

DOI:10.1080/08927018909378107

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

A simple model system was developed in order to study the effect of saliva on bacterial adhesion to a hydrophobic model surface, polystyrene. Washed bacterial suspensions ofAcinetobacter calcoaceticusRAG‐1 (ATCC 31012) andSerratia marcescensRZ were mixed with serial dilutions of clarified saliva, and the mixtures incubated in wells of untreated polystyrene microtiter plates. Adhesion was recorded spectro‐photometrically after staining of adherent cells. Saliva inhibited adhesion ofS. marcescensRZ in a concentration‐dependent manner. Of interest was the finding that saliva exerts two distinct effects on adhesion ofA. calcoaceticusRAG‐1. It inhibits adhesion at low saliva concentrations, but causes aggregation of RAG‐1 cells and stimulation of adhesion at high saliva concentrations. Extraction of saliva with hexadecane. prior to the adhesion assay, brought about an almost complete loss of the saliva‐mediated inhibition of adhesion in both strains. This same treatment, however, did not prevent the aggregation of RAG‐1 cells and the concomitant promotion of adhesion at high saliva concentrations. Initial evidence suggests that the components responsible for saliva‐mediated inhibition of adhesion to polystyrene are of low ( < 15 kD) molecular weight.

ISSN:0892-7014    

DOI:10.1080/08927018909378108

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

The early stages of bacterial settlement on 70:30 copper nickel alloy was followed by scanning electron microsocopy. Two strains of marine bacteria (Pseudomonassp andVibrio alginolyticus) isolated from polluted harbour sea water were used. The corrosion behaviour of the alloy was studied through corrosion potential measurements made in sterile and contaminated sea water. According to our results microbial colonisation of the metal surface occurs within the first 24 h for the two bacteria used. Well defined microbial colonies with localised corrosion underneath were seen by SEM after short periods of exposure. Corrosion attack seems to be closely related to passive film modification by the bacterial settlement.

ISSN:0892-7014    

DOI:10.1080/08927018909378109

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

The development of a sessile marine community of 29 animal species on mild carbon steel panels and black perspex control panels submerged off the south coast of England was studied over a two year period. There was an overall negative effect of steel on community development. Settlement by fouling species generally occurred later on steel panels, and the percent cover achieved was usually less on steel than on perspex. Significantly reduced cover on steel was found in nine species. Species differed in their response to the two materials: some. e.g. barnacles, showed much reduced cover on steel. most showed a modest reduction, but a few. e.g. some hydroids, showed occasional positive responses to steel, possibly due to reduced competition for space on steel surfaces. Differences in community composition were greatest early on and decreased towards the end of the experiment.

ISSN:0892-7014    

DOI:10.1080/08927018909378110

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

Fouling of heat transfer equipment poses a major problem in the food and especially the dairy industry. A review of recent progress made in the study and understanding of fouling and cleaning mechanisms in relation to exchangers and heat exchange surfaces in contact with milk components is given.

ISSN:0892-7014    

DOI:10.1080/08927018909378111

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

Before the construction of an Ocean Thermal Energy Conversion (OTEC) power plant, an experimental station was built near the entrance of the Bay of Brest (France) in order to perform preliminary studies. Bacterial colonisation of three substrata (stainless steel UB6, aluminium AG4 and polycarbonate filters) was observed in a continuous flow seawater system. The study was performed between May 1983 and May 1984 and included seven experiments of 15 days each.

ISSN:0892-7014    

DOI:10.1080/08927018909378112

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

The rate of irreversible protein adsorption from whole milk onto various surfaces was modeled as a function of contact surface tension, surface temperature, and time. A new kinetic parameter related to the value of contact surface tension which corresponds to a minimal interaction between bulk protein and the solid surface is introduced. This experimentally measurable parameter quantifies the influence of contact surface tension on the apparent activation energy required for irreversible protein adsorption.

ISSN:0892-7014    

DOI:10.1080/08927018909378113

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

The effect of naturally produced hydrogen sulphide on the discoloration of two copper oxide containing antifouling paints in anaerobicseawater isdescribed. In both cases discoloration can be rapid dependingon the hydrogen sulphide concentration. Rates of discoloration at different hydrogen sulphide levels are presented and the results discussed in relation to loss of antifouling activity, corrosion and aesthetic considerations.

ISSN:0892-7014    

DOI:10.1080/08927018909378114

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

Over the last four years, there has been an increasing occurrence of biofouling in pencross bent grass greens on sand based media. The major cause is the occurrence of a biologically‐synthesised horizontally‐stratified black plug layer (BPL). To detect these layers, computer assisted tomography (CAT scan) was successfully applied to an infested core. A much clearer definition of the layers was obtained than by visual observation. Bacteriological changes were also observed, with the iron‐related bacteria appearing to be a more significant component in the BPL. The iron‐related bacteria may have seen subject to a lower level of stress than isolates above and below the layer.

ISSN:0892-7014    

DOI:10.1080/08927018909378115

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor

摘要:

The strength of attachment of ascospores of six marine Ascomycotina (Amylocarpus encephaloides, Ceriosporopsis circumvestita, Eiona lunicata, Groenhiella biveslia, Halosphaeria appendiculata. Unidentified Ascomycete “A") was investigated in a Fowler Radial Flow Chamber at various times after settlement.

ISSN:0892-7014    

DOI:10.1080/08927018909378116

出版商:Taylor & Francis Group    

年代:1989

数据来源: Taylor