摘要:

SummaryA haemagglutinin present in the haemolymph of the Murray Mussel (Velesunio ambiguus) has been purified by density gradient centrifugation and elution from the specific red cell stroma. The purified haemagglutinin is one of several which behave as a homogeneous group of proteins. Amino acid analyses of these proteins indicate that they lack tyrosine and sulphur‐containing amino acids.

ISSN:0004-945X    

DOI:10.1038/icb.1970.12

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryAnin vitrocell culture system is described which allows a quantitative study of the phagocytosis of erythrocytes by haemocytes from the freshwater crayfish (Parachaeraps bicarinatus). The studies showed that specific senmi opsonins were essential for efficient phagocytosis. These results are discussed in relationship to similar findings in other invertebrates and to the evolution of vertebrate antibodies.

ISSN:0004-945X    

DOI:10.1038/icb.1970.13

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryThe changes in distribution of phosphorus involved in initiating deposition of hydroxy apatite in an experimental system based on mineralisation of rat skin have been studied.Measurement of several major phosphate fractions indicated considerable movement of phosphorus, particularly between the inorganic and organic phosphate pools of the skin. There was also an uptake of phosporus by a collagen‐rich fraction, which was related both temporally and sequentially to mineralisation. The phosphate taken up was released from the collagen fraction within a few hours if calcification did not occur. Attempts to demonstrate an inhibitory role for pyrophosphate in this calcifying system were unsuccessful.

ISSN:0004-945X    

DOI:10.1038/icb.1970.14

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryA decrease in cellularity of the bone‐marrow has been observed in sheep that consume a cobalt‐deficient diet, whether or not they are supplemented with vitamin B12. This abnormality is accompanied by a reduction in white blood cell count and in the rate of erythropoiesis following withdrawal of a large quantity of blood.Parenteral administration of cobalt has no apparent effect on the course of the malady.

ISSN:0004-945X    

DOI:10.1038/icb.1970.15

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryAn improved technique for isoelcctric focusing of proteins in polyacrylamide gels is described.In studying insulin with the procedure, mercuric chloride in trichloro‐acetic acid was used to fix the protein in the gel.One major and five minor components were demonstrated in ox and pig insulin.

ISSN:0004-945X    

DOI:10.1038/icb.1970.16

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryThe response of forearm skin and muscle vessels to periods of circulatory arrest of 1–10 min. duration has been assessed the technique of superficial and deep venous blood oxygen saturation estimations in 11 experiments on 5 subjects. In every case periods of circulatory arrest were followed by a compictious hyperaemia (rise in oxygen saturation) in both skin and muscle vessels, the response being more marked with longer durations of ischaemia. Corroborative evidence was obtained from 3 experiments in which an increased clearance of a radio‐isotope (NaI131) from forearm skin was observed during reactive hyperaemia. It is concluded that both skin and muscle vessels of the forearm are involved in the phenomenon of reactive hyperaemia.

ISSN:0004-945X    

DOI:10.1038/icb.1970.17

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryA highly protective strain ofClostridium chauvoeiwas compared and contrasted with a number of less protective strains and strain variants. This strain was found to produce uniformly smooth colonies and was highly toxic to mice and pathogenic to guinea‐pigs. It possessed an O antigen common to all the strains ofCl. chauvoeiand an H antigen which in this study was shared only by the standard challenge strain. Apart from flagella type, no single characteristic sets this strain clearly apart from other less protective strains.

ISSN:0004-945X    

DOI:10.1038/icb.1970.18

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryIn an investigation of 1825 strains of a wide variety of Salmonella serotypes and Kauffmann‐White groups, between 5 and 10% were found to produce colicin‐like antibiotics, and at least 40% produced phage; the majority were sensitive to salmonellin, and about 40% of those tested were sensitive to at least one colicin. It is suggested that some combination of these characters might be useful for exact strain identification.

ISSN:0004-945X    

DOI:10.1038/icb.1970.19

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummaryPretreatment of HEp2 cells with DEAE‐Dextran increased significantly the number of inclusions formed following inoculation with an ovine agent of theChlamydiagroup of micro‐organisms. A fivefold increase occurred when the DEAE‐Dextran was washed off before inoculation with the agent and if the DEAE‐Dextran was poured off without washing a twentyfold increase was obtained.

ISSN:0004-945X    

DOI:10.1038/icb.1970.20

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY

摘要:

SummarySera from mice injected with pneumococcal Type III capsular poly‐saccharide were titrated for antibodies by passive haemagglutination and haemolysis. The pattern of serological reactions suggested the existence of two distinct antibodies of the same specificity, one active in haemagglutination and of relatively high affinity for antigen, the other active in haemolysis and of lower affinity. These antibodies were entirely of the macroglobulin type. Titres of haemagglutinating and haemolytic activity were followed through the responses to three doses of antigen; some conditions for establishing substantial secondary responses were related to the two antibodies formed, their pre‐injection levels and the amounts of antigen administered.

ISSN:0004-945X    

DOI:10.1038/icb.1970.21

出版商:Nature Publishing Group    

年代:1970

数据来源: WILEY