摘要:

ABSTRACTThe monoclonal antibody GAP A3 detects the HLA allospecificity A3. Reactivity of the monoclonal was in exact concordance with the presence of the A3 antigen as defined by conventional alloantisera on a panel of 59 cells from individuals with well-characterized HLA antigens. Reactivity with GAP A3 segregated with HLA-A3 in a family where three of eight siblings inherited the paternal A3 antigen. GAP A3 precipitated appropriate 44,000- and 12,000-dalton bands on SDS-polyacrylamide gels under reducing conditions from an HLA-A3-positive, but not an HLA-A3-negative B lymphoblastoid cell line. Thus, by serological, familial, and biochemical criteria, GAP A3 defines the allospecificity HLA-A3.

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.87

年代:1982

数据来源: MAL

摘要:

ABSTRACTPeripheral blood and/or bone marrow lymph o blasts from 34 children and 11 adults with acute lymphoblastic leukemia (ALL) were evaluated with a monoclonal anti-la antibody and a monoclonal anti-pan T-cell antibody (T101) specific for a 65,000-dalton T-cell antigen (T65). Seventy-six per cent of cases were Ia+T65−, 20% were Ia−T65+and the remaining 4% were Ia−T65−. Anti-la and T101 reactivity were mutually exclusive and no Ia+T65+cases were identified. In childhood ALL, the Ia+T65−phenotype was associated with good prognostic factors and longer median disease-free survival than Ia−T65+patients whose clinical parameters resembled those characteristic of high-risk T-cell ALL. Included in the Ia−T65+group were three E-rosette negative cases with clinical features of T-cell disease. Our findings compare favorably with the results of other investigators utilizing polyclonal antisera and suggest that these monoclonal antibodies, which offer the advantages of monospecific standardized reagents, will prove useful in the immunologie characteri

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.91

年代:1982

数据来源: MAL

摘要:

ABSTRACTMonoclonal antibodies have been prepared against both human and bovine fibronectin. Evidence is provided which indicates that nine different antigenic determinants are recognized by the ten antihuman fibronectin monoclonal antibodies isolated. One monoclonal antibody was identified that blocked fibronectin mediated cell attachment without interfering with fibronectin binding to collagen. Sensitive ELISA assays for fibronectins derived from 32 mammalian species have been developed with the monoclonal reagents characterized in this study.

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.99

年代:1982

数据来源: MAL

摘要:

ABSTRACTMonoclonal antibodies described as reacting with particular subsets of haemopoietic cells were screened against a variety of neuronal cell lines to further investigate their true specificity. While some reagents, e.g., monoclonal anti-cALL (J5), were found only reactive with haemopoietic cells, other monoclonals, e.g., BA1, BA2, NA134, OKT6, and OKT9, also bound to neuronal cells. Further investigation into the cross-reactivity of these antibodies to a variety of neural crest derived cell lines indicated that, with the exception of OKT9, differential binding patterns to different lines were obtained. This suggests that haemopoietic cell subsets and neural crest derived tissues can share similar differentiation antigens. For monoclonals OKT9 and BA2 this observation was confirmed biochemically, showing that it is not just antigenic determinants but similar molecular weight cell surface antigens that are shared between subsets of the two major cell types. A similar analysis using monoclonal antibodies raised against human neuronal cells or cell lines again indicates that some antigens appear unique to neural tissue while others are shared by haemopoietic cell subsets.

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.109

年代:1982

数据来源: MAL

摘要:

ABSTRACTMonoclonal antibodies directed against rat kappa light chains have been generated by immunizing SJL/J mice with soluble rat immunoglobulin, followed by fusion of immune spleen cells with the P3-X63-Ag8 myeloma cell line. Monoclonal antibodies from three of these hybridoma cell lines, MAR 18.5, 80.2, and 103.6, have been extensively characterized. MAR 18.5, 80.2, and 103.6 antibodies are of the λ2a x isotype, and bind strongly to protein A, allowing easy purification. Monoclonal antibody from clone 18.5 binds equally well to Ig of both Rl-la and Rl-lb allotypes, whereas 80.2 and 103.6 antibodies selectively bind to Rl-lb. These monoclonal antibodies can be FITC conjugated for use as a second antibody in indirect immunofluorescence assays, or radiolabeled for use in radio immunoassays requiring a specific antirat x antibody. The antiallotype specific monoclonal antibodies also may be of use in the study of rat immunoglobulin genetics

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.125

年代:1982

数据来源: MAL

摘要:

ABSTRACTFour hybridomas are described which produce antibodies to the RI-1b rat kappa chain allotype. Three are rat alloantibodies of KG2b isotype, one is a mouse heteroantibody of KG2a isotype. These antibodies were labeled by incorporation of75Se-selenomethionine and their specificity determined by a plate-binding assay. Inhibition studies using partially cross-reactive sera from Asian and AustralianRattusshow that these four hybridomas detect four distinct RI-1b specificities. Differences in the slopes of the inhibition curves indicate that each of the specificities may exist in more than one form. In addition, in the form of125I-labeled antibodies, two of these hybridomas show a high degree of specificity in binding to Ig-bearing lymphocytes from spleen and lymph nodes. These hybridomas will therefore be useful not only in studies on the phylogenetic distribution of RI-1 specificities but also in replacing conventional antisera for immunochemical and cellular immunological studies.

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.133

年代:1982

数据来源: MAL

摘要:

ABSTRACTSpleen cells from mice immunized with the human lung cancer line SK-LC-3 were fused with mouse NS-1 myeloma cells. One of the hybrid clones produced a monoclonal IgM antibody (designated F-3), detected with antimouse Ig-MHA and hemagglutination assays. This antibody was completely absorbed by O red cells and completely inhibited by low concentrations of H(O) glycoproteins and hog mucin (A + H). Bombay (Oh) red cells completely failed to absorb F-3 activity even after treatment with neuraminidase. A1, A2, A1,B, A2B, and B red cells and A- and B-glycoproteins were less effective in absorbing or inhibiting F-3 activity. Other glycoproteins (including those having Leaor blood group precursor structures) showed little or no inhibitory activity. Serum from nude mice carrying F-3 hybridoma agglutinated O and A2red cells at a titer of 1:40,000 and 1:640, respectively. A1, A1B, A2B, and B red cells were agglutinated with titers of 1:80 or less. Monoclonal antibody F-3 is, therefore, highly specific for H(O) blood group determinants.

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.139

年代:1982

数据来源: MAL

摘要:

ABSTRACTMice injected with any influenza A virus develop a range of virus-immune cytotoxic T lymphocytes, some of which tend to be more lytic for cells expressing the virus used for priming (subtype-specific) while others are cross-reactive for targets infected with all influenza A viruses. Treatment with large doses of either of two monoclonal antibodies (which bind to the influenza virus hemagglutinin molecule) at 3–48 h after exposure to virus tends to selectively block the generation of the more subtype-specific cytotoxic T lymphocyte subset, although the magnitude of cross-reactive effector function may also be diminished. Inhibition that is less obviously selective is also seen for a third monoclonal antibody. A reasonable explanation for these findings is that there is afferent blockage at the level of T-cell recognition of the influenza virus hemagglutin molecule expressed on stimulator cells. The inhibitory effect of the monoclonal antibody is apparently steric. Priming with parental strain virus followed by exposure to monoclonal antibody induces a population of cytotoxic T lymphocytes which manifest decreased cytotoxic activity for target cells infected with either the parental strain virus or with a variant virus to which the monoclonal antibody does not bind. A more practical consideration is that the administration of large doses of specific immunoglobulin may interfere with the development of cell-mediated immune mechanisms. This should be considered when developing possible therapeutic protocols using monoclonal antibodie

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.149

年代:1982

数据来源: MAL

摘要:

ABSTRACTDR antigens are thought to function as differentiation antigens and to restrict immune recognition between T cells and B cells, monocyte/macrophages, Langerhan's cells, and endothelial cells. These antigens are commonly found on tissue culture lines from metastatic melanomas and tumors of lymphocyte derivation but are notably uncommon on cell lines from other malignancies. Using frozen tissue sections, a monoclonal antibody (WI 691-13-17) known to detect an epitope common to all DR alloantigens on the β (light) chain of DR antigens, and a two-step indirect immunoperoxidase technique, DR antigens were found on all metastatic lesions tested and on many primary tumors and their histogenetic precursors. The technique of using monoclonal antibodies in indirect immunoperoxidase staining of freshly frozen tissue allows individual cells to be assessed for antigen expression and presumably more accurately reflectsin vivoantigen expression than results obtained from cells selected by tissue culture methods

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.161

年代:1982

数据来源: MAL

ISSN:0272-457X    

DOI:10.1089/hyb.1.1982.1.169

年代:1982

数据来源: MAL