ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00528.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The E6 open reading frame of the human papillomavirus (HPV) 16 codes for a protein of 158 amino acids with a theoretical molecular weight of 19.1 kD. A peptide corresponding to 147–158 amino acids (NH2‐RSSRTRRETQLC‐COOH) was synthesized, coupled to haemocyanin and used for immunization of rabbits. the antibodies obtained were used for the immunohistochemical analysis of a series of 41 paraffin‐embedded biopsies including 29 cases of HPV 16‐associated cervical lesions, five HPV 6, four HPV 11, and three HPV 18‐associated genital warts. Expression of a reacting molecule (both intranuclear and cytoplasmic) could be demonstrated in 28/29 (96.6%) of the HPV 16 lesions, in 2/5 (40%) of the HPV 6, in 2/4 (50%) of the HPV 11, and in 3/3 of HPV 18 lesions. the intensity of the staining was weak in the HPV 6 and HPV 11 lesions, whereas it was classified as intense in 21/29 (72%) of the E6‐positive HPV 16 lesions. Negative staining was almost invariably (5/6, 83%) confined to HPV‐non‐cervical intraepithelial neoplasia (HPV‐NCIN) lesions, only 5/18 (27%) of which showed an intense staining. In contrast, intense staining was associated with CIN; 11/12 (92%) in HPV‐CINI and 8/11 (73%) in HPV‐CINII lesions. In the HPV 16 group, intense reactivity was more frequent in lesions shown to make clinical progression (8/9,89%) as compared with those which underwent spontaneous regression (10/15,66.7%). Topography of the immunostaining paralleled the localization of HPV DNA hybridization signals in 20/21 (95%) evaluable HPV 16 cases, in contrast to only 1/3 in HPV 18 cases and in none of the HPV 6 and HPV 11 lesions. These differences between HPV 16 and HPV 6/11 lesions in the staining with the HPV 16 E6 protein antibody might help explain at least some of the differences in their kno

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00068.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

An internal quality control system which is used in the centralized cytology laboratory of a population‐based cervical cancer screening programme in Florence is described. It includes a peer review procedure. Abnormal cervical smears are circulated among all the cytologists and a consensus on the final diagnosis is reached. This daily procedure is designed to evaluate the performance of each cytologist and of the laboratory as a whole but can also be considered a valuable training opportunity. During an 18‐month period 1197 smears were reviewed by 15 readers using a reporting form with six main categories of reporting (from ‘regative’ to ‘invasive carcinoma’), plus an ‘unsatisfactory’ category. Overall the concordance between the 15 cytologists, assessed using the kappa statistic (range 0.46–0.71; median 0.60), was good. the level of agreement increased when a weighted kappa statistic (range 0.55–0.78; median 0.68) was used. Kappa values were also calculated for specific categories and suggested an increasing concordance with increasing severity of the lesions, the categories of ‘severe dysplasia’ and ‘invasive carcinoma’ showing the highest agreement. the poor results for the ‘moderate dysplasia’ confirmed the need for combining this group with the ‘severe dysplasia’,

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00069.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Light microscope analysis of cytological smears of suspicious lesions of the oral cavity is used as a method for detecting early cancer in the oral cavity. the sensitivity of this approach can be improved by quantitative analysis of the cells in the cytological smears.We have compared the efficiency of planimetry and the Vids V system of image analysis, as quantitative methods for discriminating between normal and abnormal cells in cytological smears of suspicious lesions in the oral cavity. Both methods detected an increase in nuclear area and a decrease in cytoplasmic area in abnormal epithelial cells from dysplastic lesions of increasing severity. However, image analysis was better able to discriminate between benign and malignant cells on the basis of nuclear size. Thus the Vids V system of image analysis is more appropriate than planimetry for quantitative analysis of cytological smears from the oral cavity.

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00070.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The nuclear DNA contents of atypical mesothelial cells from five patients who had an eosinophilic pleural effusion (EPE) were studied by the use of DAPI (4′,6‐diamidino‐2‐phenylindole dihydrochloride) DNA staining. Analysis of the nuclear DNA content revealed a polyploid pattern, with a major peak in the tetraploid region. Using an immunocytochemical technique, the atypical mesothelial cells showed a positive reaction for cytokeratin. In contrast carcinoembryonic antigen (CEA) was always negative in these cells. It is suggested that the atypical mesothelial cells with EPE had a higher rate of proliferation than did the normal mesothelia

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00071.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Partial rescreening was carried out on 9633 cervical smears reported as negative by standard screening. Each slide was ‘step‐screened’ at normal speed for 30 s. Thirteen false negative smears were detected by this method. No false negatives were revealed by conventionally rescreening 10% of the same study group. the sensitivity of the method was assessed by ‘step‐screening’ 100 known positive smears. of this group 92 were detected. the results indicate that partial rescreening is a sensitive method of quality assurance and should replace conventional 10% proportional rescreening, which is

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00072.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00073.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00074.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00075.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0956-5507    

DOI:10.1111/j.1365-2303.1993.tb00076.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY