摘要:

SUMMARYIn a population of 282 Caucasoid patients in end‐stage renal failure (ESRF) Gm and Km typing has confirmed a significant increase in the frequency of the Gm1,2,17;21haplotype. This was confined to an increase in the number of heterozygous Gm(1, 2, 3, 17; 21, 5, 10, 13, 14) individuals. A concomitant decrease in the frequency of presumptive Gm(3, 5, 10, 13, 14) heterozygotes was also confirmed. Analysis of individual renal diseases revealed significant immunoglobulin allotype, phenotype and haplotype frequency deviations in those patients presenting with hypertension, glomerulonephritis or pyelonephritis. The allotype distribution in patients with physical abnormalities of the urinary‐genital tract, whether congenital or acquired, was nor

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00845.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYTwo groups of Chinese individuals of the city of Wuhan in the People's Republic of China, consisting of 98 blood bank donors and 278 workers in a steel factory, respectively, have been typed for G1m, G2m, G3m, A2m and Km allotypes. TheGm‐A2mhaplotype frequencies as well as theKmgene frequencies appeared to be quite similar in these two groups of people, the main haplotypes beingGmaf;n;bA2m2and Gmza…;gA2m1. After joining the data of the two groups, the frequencies of the mainGmhaplotypes and theA2mandKmgene frequencies have been compared with those of six other Asian populations. In Asia, going from the south to the north, an increasing frequency ofGmza;…;gand a decreasing frequency ofGmaf;n;bwere observed. The frequencies found in the investigated Chinese population lie between the frequencies found in the Chinese from Taiwan and the Jap

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00846.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYMethodologies are described for the production and characterization of monoclonal antibodies to human haemoglobin. Three monoclonal antibodies are described, two of which recognize distinct determinants on the α‐chain subunit. A third monoclonal antibody, Hb‐2d, recognizes a determinant expressed on human β‐chain. The Hb‐2d determinant is shared by human and baboon haemoglobins, but is not expressed by haemoglobins from beef, goose, pig, rabbit, sheep, dog, rat or mouse. Monoclonal antibody Hb‐2d will bind to haemoglobin A2 but not to foetal haemoglobin suggesting that δ‐ but not γ‐chain also expresses the Hb‐2d determinant. The results of testing a limited panel of human haemoglobin va

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00847.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYUsing an anti‐Pkmonoclonal antibody (mAb) designated CPK‐1, the expression of the Pkantigen was assessed on normal human tissue from non‐Pkindividuals. Although the Pkantigen was detected on fibroblasts and blood vessels as previously reported, it was also found on smooth muscle cells of the digestive tract and the urogenital system. Pkwas also found on glandular cells of the stomach, oesophagus and prostate. Additionally, CPK‐1 reacted weakly with oesophagus squamous cells, and a small number of glomeruli and tubules in the kidney. The mechanism of expression of the Pkdeterminant in non‐Pkindividuals is

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00848.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYC57BL/10 (abbreviation B10) female mice give a high primary popliteal lymph node (PLN) response to syngeneic male thymocytes. The PLN response to the H‐Y antigen is suppressed if B10 females are primed by an intraperitoneal injection of syngeneic male cells. Suppression of the response can be induced by priming with not only syngeneic B10 male thymocytes but also allogeneic thymocytes which share theH‐2Dlocus of theH‐2bhaplotype with the responder (Dbrestriction). On the other hand, B10.D2, B10.A, and HTO female mice, giving a low primary PLN response to H‐Y, give high secondary PLN responses when primed intraperitoneally with syngeneic male thymocytes. A high secondary response can also be obtained by priming with allogeneic male thymocytes which share theK/i>end loci (K, Aβ, Aα, Eβ, and Eα) of theH‐2complex with the responder. Apparently the male thymocytes used for priming must share class I (and possibly class II)H‐2loci with the female recipients to enable the recognition of the H‐Y antigen and subsequent development of the genetically determined type (suppression or amplification) of the secon

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00849.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYThe purpose of the present work was to characterize the immune response (Ir) genes that influence augmentation of the antibody response by help with the hapten azobenzene‐arsonate (ABA). Hapten help was measured as the augmentation in the plaque‐forming cell (PFC) response to bovine gamma gloulin (BGG) after priming mice with ABA conjugated to ovalbumin (OVA) and challenging subsequently with ABA‐BGG. The first approach involved inbred mouse strains that were matched forH‐2but differed in their non‐H‐2genetic backgrounds. B10.D2 mice were low responders even though they shared theH‐2dhaplotype with BALB/c and DBA/2 mice, which were high responders. C57BL/10 mice were high responders even though they shared theH‐2bhaplotype with C57BL/6, C3H.SW, and A.BY mice, all of which were strains that were low responders. The second approach was to identify any segregation ofH‐2woth the gene(s) encoding susceptibility to hapten help in the backcross generation. (BALB/c x C57BL/6) F1hybrids were backcrossed to C57BL/6. The results showed no association with the major histocompatibil

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00850.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYExperiments reported here demonstrate that the RT7 alloantigen and the L‐C antigen are separate and distinct structures on the surface of rat lymphocytes. The distribution of the antigens in different rat strains, including the mutant WF/fz, clearly establish the RT7 antigenic system as a polymorphic diallelic system, whereas the recognized L‐C antigenic determinatn is monomorphic and present on the lymphocytes of all strains tested. These data were obtained using monoclonal antibodies to the antigens in indirect immunoffluorescence experimenta. The two antigens were shown to redistribute (cap) independently of one another on the surface of rat thymocytes. Cells that had been exposed to anti‐L‐C antibody and FITC‐cojugated anti‐Ig followed by anti‐RT7.1 antibody and RITC‐conjugated anti‐Ig demonstrated FITC ca

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00851.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

SUMMARYUsing monoclonal antibodies, mouse peritoneal neutrophils were typed for the presence of 23 cell surface alloantigens, the expression of which was quantitated by flow cytofluorometry and compared with that of lymphocytes. The H‐2K and H‐2D alloantigens and β2‐microglobulin were present on all neutrophils, but Ia and Qa antigens were not detected. It was found that Ly‐5.1, Ly‐15.2, Ly‐21.2, Ly‐24.2 (Pgp‐1) and Ly‐25.1 were present on>90% of neutropils; Ly‐6.2 and Ly‐27.2 were absent, but Ly‐28.2 (encoded by anLy‐6linked gene), was present on>90% of neutrophils. As expected, the lymphocyte‐specific antigens Ly‐1.1, Ly‐2.2, Ly‐3.1, Ly‐7.2, Ly‐12.1 and Thy‐1.2 were absent from the neutrophils. When compared with lymphocytes, marked differences in alloantigen expression on neutrophils were seen for Ly‐5.1, Ly‐24.2 and Ly‐28.2. These studies should be of value in

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00852.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

HLA antigen frequencies have been determined in 282 controls and in 33 individuals with isolated cleft palate or the related birth defect velopharyngeal incompetence. No association of particular HLA antigens with this birth defect was found. A previously reported association of HLA A2 with cleft palate, in males, was not confirmed.

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1985.tb00853.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY