ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01084.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01085.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYNeonatal infection of C57BL/10 mice with cloned ecotropic and/or dual‐tropic mink cell focus‐inducing (MCF) mouse leukaemia viruses (MuLV), induces a wide spectrum of different lymphomas of T, B, and non‐T/non‐B cell types. TheH‐2complex has a marked influence on both the development of lymphoma incidence and lymphoma type. A study using the oncogenic MCF 1233 virus and a series of B10 congenic mice enabled the mapping of the following:(a) Resistance to the early development of T cell lymphomas is controlled by theH‐21‐Alocus.(b) Susceptibility to early T cell lymphomagenesis is associated with anI‐A‐regulated low anti‐MCF 1233 envelope antibody response and persistent infection of the thymus.(c) B10 (H‐2b) mice, which are resistant to early T cell lymphomagenesis induced by MCF 1233 or other MuLV isolates, have high anti‐MuLV envelope antibody responses which are I‐A‐regulated. These mice develop more B cell lymphomas late in life in contrast to the early development of T cell lymphoma in B10.A (H‐2amice. The possible response mechanisms which underlie these observations, including: (1)I‐A‐regulated immunoselection against MuLV antigens expressed by (pre) leukaemic T cells, (2) aberrant expression of class II MHC antigens on some B cell lymphomas and (3) I‐A‐regulated chronic immunostimulation of MuLV‐expressing

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01086.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYHLA‐A,B,C and DR antigen frequencies were determined in a group of 188 patients suffering from acute myeloid (AML) and acute lymphoid leukaemia (ALL). These antigen frequencies were compared with those obtained on a panel of normal individuals (n= 109) of the same ethnic origin. The significance of the differences in the antigen distribution and the strength of the associations between particular HLA antigens and the disease were then calculated. The resuks obtained show a decreased frequency of HLA‐Awl9 in the overall group of patients and the group of patients with ALL. In addition, the antigen frequency of the HLA‐B18 and DRS(DRw11) antigens was also decreased in the overall group of patients and in those patients with AML but not in the patients with ALL. The results suggest that the antigen Aw l9 may confer some degree of resistance to the development of ALL and that the HLA‐B18 and/or DR5 antigens may be resistance factors for the development

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01087.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01088.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYUltraviolet tight (UV)‐induced tumours in mice are often highly immunogenic and have unique (individually specific) antigens which cause tumour rejection in normal mice. The molecular nature of these unique ‘rejection’ or ‘transplantation’ antigens is not known. We have recently isolated a syngeneic monoclonal antibody (mAb), CP28, that recognizes a unique tumour‐specific antigen on the UV‐induced regressor tumour 1591‐RE. Further analysis revealed that the antibody‐recognized antigen represents a novel major histocompatibility complex (MHC) class I molecule. However, the relationship of this molecule to the unique T cell‐recognized antigen that causes tumour rejection remained unresolved. In this study we have explored the relationship of the antibody‐defined tumour‐specific novel class I molecule to the rejection antigen, that we have previously defined with a cytolytic T cell (CTL) clone (‘anti‐A’). Two different lines of evidence suggested a close relationship. First, it was found that random subclones of the 1591‐RE tumour expressed different levels of the CP28‐defined antigen which correlated with the level of lysis by the anti‐A CTL clone. Second, the selection of antigen‐loss variants using either the anti‐A CTL clone or the mAb CP28 resulted in the simultaneous loss of both the CP28 as well as the ‘A’ antigen. This tight correlation strongly suggests a relationship between the antibody‐defined and the T cell‐defined antigen. However, the role of the antibody‐recognized antigen in causing trans

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01089.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe C3H UV‐induced fibrosarcoma, 1591, is highly immunogenic and, therefore, is readily rejected when transplanted into immunocompetent syngeneic recipients. Previous analysis of 1591 with tumour‐specific or H‐2‐reactive monoclonal antibodies revealed that this antigenicity might be due to the expression of two novel class I major histocompatibility complex (MHC) antigens. In this report we describe the molecular cloning and initial characterization of three genes which account for all of the unique serological class I reactivities observed on this tumour. These include two distinct, but highly conserved, H‐2L‐like genes, and a third gene the product of which bears determinants which are characteristic of both the tumour and of class I products of theH‐2khaplotype. Moreover, each of these genes contains a polymorphic restriction enzyme fragment which is detected in the class I sequences of 1591 relative to normal C3H tissue. Since the expression of these polymorphic class I sequences is relevant to the immunogenicity of 1591, the mutational events by which these genes were generated may be significant to the immunobiology o

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01090.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYThe K36.16 thymoma expresses an H‐2Dd‐like allospecilicity as detected by the 34‐5‐8 monoclonal antibody directed against H‐2Ddusing immunoprecipitation techniques. This monoclonal antibody gives a negative result by radio‐immune assay but other anti‐Ddantibodies react positively with this tumour. These results confirm and extend previous observations and are attributed to probable gene‐conversion events possibly involving a donor gene from theQafTlaregion, the products of which cros

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01091.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYK36.16 is an AKRH‐2kthymoma which expresses an aberrant H‐2Dd‐like allospecificity, does not have a detectable amount of the H‐2Kksyngeneic antigen and grows very easily in syngeneic mice. By DNA‐mediated gene transfer experiments, we were able to obtain transformed clones which do express the H‐2Kkmolecules and are rejected by AKR mice. Southern hybridization was performed to assess whether any gross changes had occurred in the K36.16H‐2Klocus or elsewhere in the MHC, which might explain the lack of H‐2K expression and/or the presence of the aberrant H‐2Dd‐like allospecificity. Specific H‐2 class I DNA probes were used to compare the K36.16 genomic DNA with normal AKR thymus DNA after digestion with a variety of restriction enzymes. After hybridization with the pH‐2IIa probe a 2.8 kb ‘Hind III’ fragment was identified in the K36.16 genomic DNA which is absent from AKR DNA. The pH‐2IIa probe detects the third, transmembrane and cytoplasmic domains of class I genes. Although these changes are indicative of MHC genome modifications it is not yet possible to link these specific Southern blot pattern variations with the pheno

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01092.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

SUMMARYFrom a series of mouse sarcomata, newly induced by Rous sarcoma virus (RSV), the DOH cell line was shown to lack expression of syngeneic H‐2Kdand Dkantigens (No11et al., 1986). It unexpectedly displayed determinants specific for H‐2Kkmolecules. Interferon treatment of DOH stimulated the expression of H‐2Kkdeterminants and also the display of some, but not all, determinants of the syngeneic H‐2Kdmolecules. H‐2Dkexpression was not stimulated. Southern blot hybridization of genomic DNA digests from DOH cells confirmed the identity of theH‐2Kregion with that from syngeneic C3H.OH liver cells, but also showed changes in the pattern of restriction fragments that contain class I genes from theDandQaregions. These results suggest that aberrant MHC class I molecules that carry H‐2Kd‐ and H‐2Kk‐like determinants are expressed by DOH sarcoma cells. These molecules may act as target antigens for turnour‐specific cytotoxic T cells elicited by injection of DOH cell

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1986.tb01093.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY