ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00702.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryExpression of H‐2 antigenic specificities on K36, a spontaneous leukaemia originating from AKR (H‐2k) mice, was studied by serology and immunochemistry. Two ascites lines of the tumour, as well as a tissue culture adjusted and cloned tumour line, were used in these studies with similar results being obtained.K36 expresses on its cell surface D‐region encoded H‐2K antigens but does not express K‐region encoded H‐2K alloantigens. It also expresses on its cell membrane, H‐2 specificities of foreign haplotypes not present on normal AKR lymphoid cells. The molecular basis of the H‐2Ddspecificity on K36 (H‐2kwas analysed by immunoprecipitation and polyacrylamide gel electrophoresis. The specificity was shown to be present on a glycoprotein of apparent molecular weight 45,000. However, antisera against the H‐2Ddprivate specificity (H‐2.4) precipitate additional glycoprotein of 45,000D and also 70,000D.In tryptic peptide maps of the isolated 45,000D fraction precipitated by anti‐H‐2.4 serum from radiolabelled K36 glycoprotein, all H‐2Ddspecific peptides were present in the same quantitative ratio. This is consistent with the structural identity of the foreign H‐2Ddfrom the K36 tumour with normal H‐2Ddand supports the hypothesis of a regulator system controlling theH‐2allelism. Under certain circumstances such a system could cause suppression of one and derepres

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00703.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryCellular cytotoxicity experiments were done to test the potential of the extra H‐2 antigenic specificities on the K36 spontaneous leukaemia as targets. In addition, experiments were performed to rule out the possibility that the target determinants could be normal cross‐reacting alloantigens, e.g., T1, Qa, non‐H‐2 and previously undetected H‐2 public specificities, which are more readily detectable on tumours than on normal cells.We used F1hybrid mice, in which one parent was of the strain of origin of the tumour (K36) and the other parent of the B10 congenic series, i.e., (AKR x B10)F1. These cells were stimulated by lymphoid cells from other B10 congenic strains, B10.A and B10.D2, and tested against the test tumour K36 and several PHA blast controls. Several K36 sublines as well as a cloned line of K36 (K36.16) were used and significant cytotoxicity against. an H‐2d‐like target on these tumour cells was obtained. These data exclude the possibility of a corss‐reactive alloantigen, e.g., undetected H‐2 public specificity, or differentiation antigens. These results with the K36 tunour were consistent with our immunochemical studies (see Schmidt&Festenstein, 1980) and were confirmed and extended by cold target inhibition experiments. In these experiments, B10.BR cells were sensitized by B10.D2 lymphoid cells and tested against B10.D2 (51chromium‐labelled PHA balsts). Two kinds of normal unlabelled lymph node suspensions as well as the K36.16 tumour cell suspension were used. Significant specific inhibition of between 19% and 40% was obtained using K36 and between 23% and 37% using B10.D2 (positive conrol). AKR cells (negative control) in contrast were unable to reduce the precentage specific cytotoxicity.Since it was already known that theH‐2Kkgene products are missing from this tumour (Schmidt&Festenstein, 1980), it was of interest to test whether cytotoxic effectors directed against theH‐2kkgene products were able to kill the K36 tumour. Accordingly, B10.D2 lymphoid cells were sensitized to B10.BR (C3H.0H) and B10.A targets, respectively, and tested against K36 and appropriate controls. Only weak killing was observed when sensitization was effected against the K end of theH‐2khaplotype (i.e., using B10.A as the sensitizing cell) but strong and significant cytolysis was found when the sensitization was against the wholeH‐2khaplotype or against the H‐2Dkgene product. These results were confirmed by cold target inhibition studies.These experiments provide further indications for the H‐2d‐like characteristics of these allodeterminants. We have already excluded some of the possible explanations for these findings (i.e., corss‐reactions with H‐2 and non‐H‐2 normal specificities). The cold target inhibition experiments rule out non‐specific viral effects. Thus, we favour an alteration in regulatory genes leading to repression of theH‐2Kkproduct and derepression of theH‐2Ddproduct, but cannot formally rule out highly corss

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00704.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryThe K region of the H‐2 major histocompatibility complex (MHC) of mice ofH‐2Kkhaplotype codes for two distinct alloantigens. One of these alloantigens, designated k‐common, is expressed by C3HfB/HeN mice (C3Hf). The other alloantigen, designated k‐unique, is not expressed by C3Hf mice. TheH‐2haplotype of C3Hf mice has been classified as kv1 and the variant antigen distinguishing this strain from mice ofH‐2Kkhaplotype has been designated kv1‐unique. Several transplacentally‐induced lung tumours of C3Hf mice express the k‐unique, rather than the expected kv1‐unique, antigen. The immunogenicity of the k‐unique antigen on C3Hf‐derived lung tumours varies with different tumours. In particular, the capacity of the k‐unique antigen to induce radioresistant immunity in C3Hf mice appears to be lost on long term cultured tumour cells even though the tumour remains susceptible toin vivoimmune responses directed against the k‐unique antigen. Alterations in expression and in immunogenicity of unique H‐2‐coded antigens may dictate the nature and efficacy of immune survei

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00705.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryThe inbred mouse strains BN/a and BN/b have haplotypeH‐2bpcharacterized by H‐2.33 and lacking any other private specificity known in inbred strains. Two transplantable B cell leukaemias which originated in BN/a and BN/b mice treated with anti‐lymphocyte globulin were tested serologicaly for H‐2 antigens. Tests during passages 169‐181 revealed several quantitatively different reactions with sera against public specificities, some of these being due to Ia antibodies. No change in expression of the private specificities was seen. On the other hand, during the later passages (239‐258) a number of qualitative differences were seen, the most remarkbale being the loss of H‐2.33 and gain of H‐2.4, 31. The overall serological pattern of cells resembled that ofH‐2drather than ofH‐2bphaplotyes and this was confirmed by absorption tests. The changes reported here may be due to alterations of repression and derepression pattern of the presumed multiple structuralH‐2genes

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00706.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryAlien H‐2k‐like antigens were found to be expressed by a methylcholanthrene induced tumour of BALB/c (H‐2d) origin. H‐2 specificities of thekhaplotype were detected on this tumour by a variety of serological techiques, including51Cr‐release cytotoxicity, microradioassay and absorption. The antisera employed were conventional polyspecific alloantisera, typing sera with restricted specificty and monoclonal hybridoma‐derived anti‐H‐2kantibodies.The tumour has a low expression of the private specificty 31, which characterizes Kdmolecules, and does not seem to express the private specificities of Dd, Kkand Dkmolecules. It appears to express predominantly alien H‐2‐like antigens which are very similar to but not identical with n

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00707.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryThe immune response of SJL/J (H‐25) mice to syngeneic reticulum cell sarcoma (RCS) tumour cells was investigated. The nature of tumour‐associated antigens was examined by immunological and biochemical techniques. Reticulum cell sarcomas of different origins (spontaneous, transplantable and cultured cell lines) stimulate a strong syngeneic response measured by [3H]‐thymidine incorporation. However, only the cell line were able to stimulate a syngeneic cell‐mediated cytotoxic response. Further analysis of the syngeneic response revealed that RCS cells express inappropiate alloantigenic specificities on their surface. Thus, evidence is provided which demonstrates that RCS tumour cells carry antigens which cross‐react with BALB/c (H‐2d) and C57BL/6 (H‐2b) alloantigens. The presence of inappropriate antigens in thein vitrolines was detected by cell‐mediated cytotoxicity, cytotoxic antibody and immunofluorescence whereas antigens onin vivolines and spontaneous RCS were detected primarily by immunofluorescence. Conversely, H‐25expression on thein vitrolines was detected only by immunofluorescence whereas the H‐25of thein vivolines were detected by cytotoxic antibody. By these assays inappropriate antigens were not detected on normal SJL/J lymphocytes. Immunochemical analysis of the inappropriate antigens by two‐dimensional gel electrophoresis was performed with SJL/J anti‐BALB/c and monospecific anti‐H‐2Ddsera. These sera precipitated 45,000 MW molecules from BALB/c lymph node cells and from SJL/Jin vivoandin vitroRCS tumour which appeared very similar. The sera also precipitated molecules from SJL/J lymph node cells which resemble the BALB/c and RCS molecules. Thus, RCS tumours express inappropriate alloantigens which may be cryptic on normal cells. The biological significance of these tumour‐associated antige

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00708.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummarySerological and structural changes of surface markers involved in immune reactions may occur in human and murine tumour systems. Thus nine out of twenty‐one human tumour cell lines and SV40‐transformed fibroblasts differed from autologous lymphoblastoid cells or fibroblasts in their reactivity with HLA allonatisera. H‐2 antigens isolated from the murine tumour cells 6C3HED and TP 1422, undergo structural changes. An alien HLA‐B7 was detected in sera from two melanoma patients. The serologic activity on H‐2 antigens was significantly increased in the serum and ascites fluid of tumour bearing mice. Additionally, human SV40‐transformed fibroblatsts acquire receptors for monkey red blood cells and the murine lymphosarcoma cells 6C3HED express receptors for sheep red

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00709.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryGenes in the D region of the murine major histocompatibility complex, H‐2, confer resistance to radiation‐induced leukaemia virus.H‐2Dgene control appears to ensue at a step subsequent to virus infection, since elimination of virus infected cells does not become apparent until 3‐5 weeks after virus infection. Nonetheless, almost immediately after virus infection, expression of H‐2D‐coded antigens is markedly elevated on the surface of thymocytes from resistant (H‐2Dd) but not susceptible mice (H‐2Dsor H‐2Dq). This increased H‐2D antigen expression triggers a vigorous cell‐mediated immune response which probably plays a key role in resistance to leukaemia via elimination of virus‐infected cells. A hypothesis is put forth to explain the induction of increased sythesis and expression of H‐2D antigens. This hypothesis postulates that the oncogenic segment of RadLV bears a close resemblance to H‐2.4, the private specificity for H‐2Dd, allowing it to integrate at or near theH‐2Ddmurine gene. Subsequent to integration, the rates of transcription and translation are altered with a resulting increase in cell surface antigen expression. Other p

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00710.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

SummaryWhen testing the serum of an individual anti‐H‐2 immunized mouse (B10 x A.SW)F1anti‐B10.M by the routine micro‐lymphocytotoxicity test on lymph‐node cells, unexpected antibodies were found. The most striking finding was that after absorption of anti‐H‐2.8 antibodies with B10.A(2R) (Kk) cells, antibodies remained which reacted with AKR, B10.AKM and B10.A V + mice while B10.A V‐, B10.BR and C3H mice were negative. While all these strains share theKkallele, only the positively reacting strains express high titres of infectious RNA turnover viruses. Unexpected reactions were observed also with H‐2d, H‐2jand H‐2rcells and absorption experiments indicated two or three antibody populations.These reactions could be interpreted by two different possibilities: (1) anti‐H‐2 antibodies react with virus‐altered H‐2 structures; and (2) antiviral antibodies react with H‐2 structures complexed with viruses. These possibilities should be taken into account when H‐2 sera are tested

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1980.tb00711.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY