摘要:

SUMMARYSerolgoical and immunochemical assays detected antibodies to human la‐like antigens in five out of five rabbit antisera to cultured human B lymphoid cells but did not detect them in five bleedings from two rabbits immunized with human peripheral blood lymphocytes. These results, which may reflect differential immunogenicity of la‐like antigens from different cells, indicate that the presence of anti la‐like antibodies in heterologous antihuman lymphocyte sera is not a general phenomenon as reported recently for animal model sy

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01067.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe factorial correspondence analysis, which is a descriptive statistical method, was used to study the preferential associations between locus HLA‐A, B, C and Bf alleles. This method does not limit the number ofloci that can be studied and must therefore be considered as an interesting method of studying haplotypic associations within the HLA syste

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01068.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYThe murine Ly‐4 locus is further described with the definition of both Ly‐4.1 and 4.2 specificities and the demonstration of linkage ofH‐3 on Chromosome 2. As has been found for several other Ly‐specificities, both Ly‐4 specificities are absent from the thymus, but are found on peripheral T and B cells, with B cells having the greatest antigen expression. The expression of both Ly‐4 specificities was influenced by an H‐2‐linked gene, mapping in or near

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01069.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYAn alloantiserum prepared against LyM‐1 antigen of the mouse has been analysed in detail. This antiserum, C3H/HeJ anti‐CBA/J, has turned out to define two antigens which are specified by independently segregating loci. One of these is Lyb‐2.1 or similar antigen specified by a gene located on chromosome 4. The other is identified as LyM‐1.2 based on the result that a gene determining alloantigenicity in the Lyb‐2‐strain is closely linked toMlswith recombination frequency of 0.066 ± 0.028. Since both antigens are expressed preferentially on B lymphocytes and their strain distribution is significantly overlapped, there has been much confusion about LyM‐1 antigen. This is overcome by re‐evaluating anti‐LyM‐1.2 serum under the condition in which Lyb‐2.1 is not involved in analysis. The strain distribution of LyM‐l antigen, its preferential expression on B lymphocytes and close genetic linkage ofLyM‐l

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01070.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYMph‐1 is an alloantigen demonstrable on mouse peritoneal macrophages. Two lines, B10M.I and C.I, have been bred for use with strains I/StDa or BALB/c to produce appropriate alloantisera. Optimal immunization procedures for production of anti‐Mph‐1.2 (the common antigen) are described. Antisera against Mph‐1.1 have so far been obtained only erratically.Antisera show complement dependent cytotoxicity against normal peritoneal macrophages and cultured blood monocytes but have no obvious effect on peritoneal lymphocytes or cultured blood neutophils. Absorption studies show that cell for cell normal peritoneum has about fifteen times more antigen than lymph node, spleen, liver or bone marrow and at least eighty times more than thymus. We were unable to use the antiserum to cause passive enhancement of skin grafts

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01071.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYHaemagglutination inhibition studies using isolated membrane components from rat red blood cells showed that the activity of the major antigenic system RT2 was contained exclusively in the glycoprotein fraction and confirmed immunochemically the existence of two alleles,RT2aandRT2b. The SDS‐PAGE analysis of the membrane showed two interconvertible glycoproteins of apparent molecular weight 72,000 and 36,000, and the higher molecular weight component appeared to be a dimer of the lower molecular weight one. Analysis of the various membrane preparations after125I‐labelling showed that the glycoproteins existed in at least two molecular species on the surface of the red cells and that one species was more readily radio‐iodinated and dimerized than the other. The RT2 antigenic specificities were preferentially associated with this comp

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01072.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYA cloned hybridoma, producing an IgM kappa antibody, has been derived from a fusion between NSI myeloma cells and spleen cells from BALB/c mice immune to CBA lymphocytes. Its specificity for Lyt‐1.1 alloantigen has been established from (1) the lymphoid tissue distribution restricted to T cells and (2) the reactivity pattern on a panel of thymocytes from standard and Lyt congenic mouse strain

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01073.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

SUMMARYSpleen cells from C3HfB/HeN mice (H‐2kv1) were markedly less susceptible to lysis by monoclonal anti‐Kkantibody (Clone 11‐4) than spleen cells from C3H/HeN mice (H‐2k). Less than 50% of C3HfB/HeN cells were lysed even at the highest antibody concentration tested compared to 70% of C3H/HeN cells. The difference in end point titre of the antibody tested on cells from the two strains was 5‐10‐fold. Similar differences were not observed using the conventional H‐2Kk‐typing a

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01074.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY

摘要:

F. H. BACH, B. BONAVIDA, ELLEN VITETTA, AND C. F. Fox; T and B lymphocytes: recognition and function. Academic Press, New York, 1979. ISBN 0‐12‐069850‐1, Price U.S. $

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1981.tb01075.x

出版商:Blackwell Publishing Ltd    

年代:1981

数据来源: WILEY