ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00365.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYBiosynthetically labelled Qa‐2 antigens were isolated from mouse spleen cells by immunoprecipitation with anti‐Qa‐2 antisera. When newly synthesized Qa‐2 molecules from several different inbred strains were analysed by two‐dimensional (2D) gel electrophoresis; four different phenotypes were observed that differed in the number of polypeptides present. The ability to distinguish Qa‐2+phenotypes was used to map the recombination points in two congenic strains, B6.TlaaandA. Tlab. No alternative Qa‐2‐like polypeptides were detected in B6.K1 (Qa‐2−) cells using a polyspecific rabbit antiserum against mouse class I antigens, but a new molecule was detected in BALB/cBy (Qa‐2−) cells. Pulse‐chase and surface‐labelling experiments showed that some, but not all, of the newly synthesized Qa‐2 precursor forms are processed to

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00366.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYCareful genotyping of three families, each having a member with classical salt‐losing steroid 21‐hydroxylase deficiency, has allowed identification of carrier haplotypes. Digestion with TaqI or EcoRI and probing with a cDNA probe for the 21‐hydroxylase genes (pC21/3c) revealed that all six affected haplotypes are abnormal with at least EcoRI. The data suggest that there is extreme polymorphism of the 21‐hydroxylase genes and that dysfunction may result from several different abnorm

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00367.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00368.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYAntigen‐specific helper factor (ASHF), a soluble product of T helper (Th) cells, binds antigen and can induce B‐cell and cytotoxic T‐lymphocyte (CTL) differentiation. Its relationship to the T‐cell surface antigen receptor (TcR) is unknown. Both have MHC‐restricted recognition of nominal antigen, thus they may share very similar combining sites. Using monoclonal anti‐TcR to imrnunoprecipitate partially purified ASHF, we have obtained evidence for shared determinants between ASHF and the TcR. Antigen affinity‐enriched supernatants of a Th clone, LB19, are functionally active in antigen‐specific, help‐dependent CTL assays. FPLC anion exchange salt fractions of these supernatants were125I‐labelled and immunoprecipitated with KJ16.133 monoclonal anti‐TcR coupled to Sepharose 4B. Precipitates were analysed by SDS‐PAGE. We have obtained clear evidence that functionally active Th culture supernatants contain molecules specifically precipitab

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00369.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYAntigen‐specific T‐cell helper factors were secreted from a (T,G)‐A—L specific T‐cell line and clones. The factors were released upon antigenic stimulation and could be induced by a low or a high dose of antigen. The factors secreted upon low‐dose stimulation possessed the antigenic specificity of the secreting cells, while the high dose‐induced factors had a broader antigenic specificity and could react with the closely related polypeptide (Phe,G)‐A—L, even when the cells were restricted to (T,G)‐A—L. Both the low dose‐ as well as the high dose‐induced factors could not trigger antibody production in the presence of a non‐relevant antigen, and did not collaborate with B cells immunized with a non‐related antigen for the production of antibodies. The helper factors, like their secreting cells, wereH‐2‐restricted in the collaboration with B cells. In contrast to the helper cells, however, they did not require accessory cells for triggering the B cells in the process of antibody production. Some preparations of helper factors were found to be inactive. The helper activity could be restored by IL‐2. Thus, IL‐2 is an additional essential factor required for the antigen‐specific collaborati

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00370.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARY(CBA × B10)F1[(H‐2kxH‐2b)] mice produce two types of antigen‐specific T‐suppressor factor (TsF), which can be separated by affinity chromatography on anti‐I‐J monoclonal antibody. After reduction and alkylation, both chains of F1TsF are required for biological activity. However, the antigen‐binding chain (AgBC) of F1TsFk(AgBCk) is only complemented by I‐Jkand likewise for F1TsFb. In other words, interchain complementation shows the same genetic restriction in interchain complementation in parental and F1mice. F1TsF bearing, for example, I‐Jk(TsFk), interacts with haptenized ‘antigen‐presenting cells’ (‘APC’) of both parental haplotypes, and may be described as showing dual reactivity. In contrast, it is known that parental TsF shows single reactivity, and only interacts with haptenized ‘APC’ of the parental haplotype. The combination of F1AgBCkand parental I‐Jkchains interacts with haptenized ‘APC’ of both parental haplotypes (dual reactivity). In contrast, the combination of parental AgBCband F1I‐Jbshows single reactivity and only interacts with haptenized ‘APCb’. It was inferred that the antigen‐binding chain is responsible for the dual reactivity and, hence, for the interaction with a member of the I‐J hierarchy on the ‘APC’. It was concluded that the antigen‐binding chain of TsF has two recognition sites for members of the I‐J hierarchy—one for interchain complementation and the other for interaction with the haptenized ‘antigen‐presenting cell’. The termmember of fhe I‐J hierarchyis used instead of I‐J because it is not clear whether the AgBC interacts wi

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00371.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00372.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYSerum IgG antibodies to ovalbumin (OA) and beta‐lactoglobulin (BLG) were quantified by ELISA techniques in 22 monozygotic (MZ) and 24 dizygotic (DZ) healthy twin pairs. Antibody levels were comparable in the MZ and DZ groups both for anti‐OA and anti‐BLG antibodies. The genetic variance (ĜWT) was 0.167 for log IgG anti‐OA antibodies, and 0·173 for log IgG anti‐BLG antibodies, with heritability estimates of 0·44 and 0·37, respectively. No indication was observed of genotype–environmental interaction or differential environmental covariance for the log antibody levels in the MZ and DZ twins. The anti‐OA and anti‐BLG antibody levels in the same individual correlated only to a low degree. The levels of naturally occurring serum IgG antibodies are significantly influenced

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00373.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

SUMMARYAn association of HLA‐DR5 and goitrous autoimmune thyroiditis has been reported elsewhere (Faridet al., 1981; Weisselet al., 1980). Recently, the disease was found to be associated with HLA‐DR4 in Newfoundlanders (Farid&Thompson, 1986). In order to find out whether different HLA associations with the disease may be found in different ethnic groups, we have now typed 68 patients with autoimmune goitrous thyroiditis from Eastern Hungary for HLA‐A, ‐B, ‐C, and ‐DR antigens; 66 of these patients were also typed for IgG heavy‐chain markers (Gm). A significant increase in DR3 (OR = 3·30) and a non‐significant increase in DR4 (OR = 1·67) were found in the patients when compared with controls. The Gm3 allele, g, interacted with DR3 to enhance the risk for goitrous autoimmune thyroiditis. Hashimoto's disease may show different associations in different ethnic groups, and indeed within the same ethnic group, when newly diagnosed patients are typed se

ISSN:1744-3121    

DOI:10.1111/j.1744-313X.1987.tb00374.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY