摘要:

SUMMARYKidneys were transplanted from Lewis and Brown Norway F1hybrid rats to Lewis recipients, and glomerular alterations were examined serially by light, electron, and immunofluorescence microscopy. Glomerular hypercellularity develops 3-5 days after allografting. Granular deposits of immunoglobulin G (IgG) and β1e-globulin (C3) occur at this time on glomerular capillary walls and mesangial areas, and subepithelial “humps” of basement membrane-like material are located on glomerular capillaries. The glomerular IgG and C3 deposits are short lived. Numerous polymorphonuclear leukocytes appear within glomeruli, strip away endothelium from basement membranes by extending pseudopods between endothelial cytoplasm and basement membrane, and accumulate IgG and C3 within their cytoplasm. On day 7, when renal function ceases, glomeruli are hypocellular and glomerular capillaries are occluded by platelets and fibrin. The results suggest (1) development of an immune complex glomerulitis during acute allograft rejection and (2) fibrin deposition during allograft rejection.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYEnhanced growth of isografts of a benzpyrene-induced sarcoma (BP-4) was produced in C3H mice by mixing tumor cells in vitro with normal syngeneic spleen cells which had been preincubated with ribonucleic acid (RNA) preparations (BP-4 RNA) extracted from the lymph nodes and spleens of guinea pigs previously immunized with cells from the same sarcoma. RNA from pigs immunized with normal C3H tissues was significantly less effective in mediating enhancement, while RNA from pigs immunized with only Freund's adjuvant was totally ineffective. The response was abolished when active RNA preparations were treated with ribonuclease (RNase). Sera were obtained from mice receiving mixtures of tumor cells and spleen cells which had been preincubated with BP-4 RNA. Such sera contained enhancing antibodies, as evidenced by the enhanced growth of tumor cell inocula when tumor cells were incubated with these sera in vitro prior to their injection into susceptible hosts. The enhancement was specific for the immunizing tumor, since BP-4 RNA mediated enhancement of BP-4, but not BP-1, isografts (a second benzpyrene-induced sarcoma in C3H mice), and BP-1 RNA mediated enhancement of BP-1, but not BP-4, isografts.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYAn apparent histoincompatibility was demonstrable between mice chronically infected with lymphocytic choriomeningitis (LCM) virus and their uninfected syngeneic counterparts. Transplants of whole skin from infected donors to uninfected recipients were consistently rejected within 12-14 days. Also rejected were transplants of single cell suspensions of a histocompatible adenocarcinoma when they were transferred from infected donors to uninfected recipients. Using infected cells or debris from infected cells as challenge antigens, a delayed-type of hypersensitivity reaction could be evoked in animals that had previously rejected infected tumor transplants or which were otherwise immunized with the virus. Lymph node cells harvested from mice previously injected with LCM virus were shown to have a destructive action upon mouse L cells which were chronically or acutely infected with the virus. It is suggested that cells chronically or acutely infected with LCM virus exhibit new antigenic determinants on the surface of the cytoplasmic membrane. An immune response directed against these new antigens might inflict irreparable damage upon such infected cells.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYThe survival of rabbit skin allografts was significantly prolonged for up to 22 days following treatment of the skin grafts with electrophoresis prior to transplantation. No relationship between one combination of current and time and a maximum survival time could be established. A new method of window electrophoresis is presented, and its possible advantages over regular paper electrophoresis are discussed.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYRabbits were sensitized with multiple sequential skin allografts and then challenged with kidney grafts. Rejection, characterized by its rapid tempo and by accumulation of polymorphonuclear neutrophils and/or renal cortical necrosis, occurred in all 12 animals which received renal grafts, against which they had the corresponding transplantation antibodies, detectable by means of the mixed agglutination test with cell cultures. Four grafts were in situ long enough for renal cortical necrosis to develop. The necrosis involved the entire outer cortex, but only an occasional glomerulus contained a few strands of fibrin. For comparison, the generalized Shwartzman reaction (gSr) was induced by infusion of ehdotoxin into seven normal rabbits. Here, the renal cortical necrosis was characterized by the sparing of some nephrons in the outer cortex and by occlusion of all of the glomerular capillaries in the necrotic areas. In the grafts, lipid stains revealed large amounts of fat in the interlobular arteries but none in the glomeruli, while in the gSr only the glomeruli were involved, suggesting different levels of vascular obstruction in the two lesions. Circulating fibrin degradation products were present in only one of eight grafted rabbits but were present in all seven rabbits with the gSr. All eight grafts placed into rabbits which did not have the corresponding antibodies showed rejection characterized by a mononuclear cell infiltration but no significant fibrin deposition or fat accumulation. Circulating fibrin breakdown products were detected in none of these rabbits. Finally, kidney allografts were given to nine normal rabbits. The production of the gSr in these rabbits at a time when the graft was in the process of rejection showed that such grafts were less vulnerable to the noxious effects of an endotoxin infusion than were the recipients' own kidneys.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYAdult, nonirradiated C57BL/6J {H-2bfemale mice do not show splenic enlargement following the injection of allogeneic (H-2k) spleen cells. Adrenalectomized, noninjected controls show splenic enlargement at 7, 14, and 21 days after surgery. When adrenalectomized mice are injected with allogeneic spleen cells, the spleens do not enlarge by the 7th day but, by the 14th day, splenic enlargement exceeds that seen in noninjected, adrenalectomized controls. By the 21st day, the spleens, although still enlarged, do not exceed in weight those of the noninjected adrenalectomized controls. Both of these effects —splenic enlargement following adrenalectomy and modification of this enlargement by injection of allogeneic cells—are abolished by injection of cortisone acetate. It is suggested that adrenalectomy may modify the host-versus-graft reaction of mice.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYA technique for the in vivo microscopic study of functioning cardiac homografts in Syrian golden hamsters has been developed, and a study of the sequence of microcirculatory and cellular events occurring in these grafts is reported. Observations indicate that cardiac grafts are vascularized initially by endothelial buds from the graft tissue itself moving outward and establishing connections with the host vessels. Grafts derived from fetal and newborn donors showed functional activity more frequently than did those derived from adult animals. Similarly, grafts derived from atrial tissue showed contractile activity more often than did ventricular grafts. The hamster cheek pouch technique permits greater resolution of the cellular and microvascular phenomena associated with cardiac graft survival than did previous methods.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYA panel of inbred mouse strains selected for their low incidence of common ancestrywas typed for the alleles of theH-1, H-3, H-4, H-7, H-8, H-9, H-12andH-13histocompatibility loci, using histogenetic techniques. As in a previous study, typing revealed new alleles at all loci with the exception of H-l. Because of the inability to type for the null antigen of the H-1ballele, we could not distinguish between strains possessing H-1band those possessing new alleles. In view of the rather high frequency of new alleles (66%), it seems likely that a high degree of genetic polymorphism exists at these histocompatibility loci. The strengths of the non-H-2 antigens varied from the relatively strong H-4b antigen to the extremely weak H-4a antigen. The immune response of the 13 F1groups was rather tightly grouped. No one appeared particularly strong or weak as compared to the group as a whole.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYRenal transplantation was performed between two strains of rat (AS and AS2) known to differ at the major histocompatibility locus, and the results were compared to those of skin grafting. Skin grafts exchanged between the strains were rejected rapidly, as were AS2 renal transplants made to rats of the AS strain. Transplantation of AS kidneys to AS2 rats gave very different results: 70% of the recipients survived for more than 50 days with good renal function and minimal evidence of rejection in their transplants. Shorter survival times were seen, however, when AS kidneys were transplanted to AS2 rats previously sensitised toward the renal donor. Long surviving AS2 recipients of AS renal allografts accepted subsequent AS skin grafts and it has been suggested that these AS2 recipients might have become immunologically tolerant of the donor strain.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID

摘要:

SUMMARYAutologous bursal implants located within the peritoneal cavity did not repair the deficiencies in germinal center production or primary antibody responsiveness of chickens bursectomized at hatching. Similarly, bursal implants had no effect on the hypermacroglobulinemia of bursectomized birds. Serial observation of bursal implants revealed early central necrosis, followed by vascularization and subquent morphological and functional integrity of bursal epithelium. Continued lymphopoiesis was not observed in bursal implants; the lymphoid follicular nature of the bursa was entirely lost within the first few days following implantation. Thymus implants exhibited early central necrosis but redeveloped normal lymphoid architectural features in the same peritoneal location. It is postulated that proximity to the contents of the intestinal lumen is essential to the special lymphopoietic functions of the bursa.

ISSN:0041-1337    

出版商:OVID    

年代:1971

数据来源: OVID