ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYThe relationship of cell-mediated immunity to transplantation antigens and clinical graft rejection was studied in inbred Wistar rats using an in vitro capillary tube migration- inhibition technique. Spleen cells from animals sensitised by grafting were inhibited by donor strain (AGUS)-derived antigens but not by recipient strain (Wistar)- derived antigens. Inhibition became significant 24-48 hr before the onset of clinical graft rejection. In a comparison of the response of skin-grafted animals to a small number of heart-transplanted animals, no difference was seen in the onset of sensitisation.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYCertain antihuman lymphocyte sera raised by immunization of horses with whole thymus homogenate contain antiglomerular basement membrane (GBM) antibody and cause severe glomerulonephritis in nonhuman primates. This study was done to characterize this antibody and attempt its removal with isolated human GBM. Injection of radiolabeled antilymphocyte globulin (ALG) into rats revealed that 0.9% bound to kidney, 0.3% to liver, and 0.1% to spleen. Immunofluorescence studies showed antibody bound to the GBM in the kidney, around central veins and along sinusoids in the liver, and along sinusoids in the spleen. There was no uptake by the thymus. Use of a species with larger renal mass did not increase the percentage of antibody bound to kidney (KFAb). In contrast to in vivo studies, 5% of radiolabeled ALG was bound by GBM in vitro. When ALG was absorbed with human GBM, it no longer bound to kidney in vivo, as detected by immunofluorescence, and the KFAb content was reduced to less than 0.005%. However, its lymphocytotoxicity was retained. The results show that the anti-GBM antibody in this ALG has broad connective tissue specificity, is readily absorbed by GBM in vitro, and probably arose in response to connective tissue in the thymus homogenate used for immunization.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYIn a system of renal allotransplantation across a major histocompatibility barrier it is possible to induce enhancement by active immunization using a single i.v. injection of cells from the thymus, spleen, lymph nodes, and whole blood, when given under dosage and timing conditions previously described for bone marrow. When using bone marrow cells, the i.v. or i.p. route is preferable to the s.c. or oral route and frozen thawed cells are ineffective. Genetic analysis reveals that a significant number of allografts are actively enhanced by immunization with reciprocal backcross and F2bone marrow cells, suggesting that the products of only 1-2 histocompatibility loci are involved in producing renal allograft enhancement during the period of usual acute rejection.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYC57BL/6J skin grafts enjoy a prolonged survival on CBA/J recipients, if the C57BL/6J donor is pretreated with high titer recipient antidonor serum. This form of immunological enhancement differs from classical immunological enhancement because the immune serum is administered to the donor rather than the recipient. To distinguish the two, the term “donor enhancement” has been used. Nonspecific immunosuppression of the donor with azathioprine or heterologous or isologous antilymphocyte serum fails to produce a similar prolongation. Moreover, a prolongation is seen even in the presence of a simultaneous ongoing rejection response.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYA study of long-term survival of rat renal transplants following cyclophosphamide (CY) treatment is reported. In the experimental model of LBNF, hybrid to L, CY, given as a single i.p. injection on day 2 or 3 post-transplant, best preserved function of the renal allograft. To obtain long survival of recipients, 21 days of CY treatment was better than an equivalent single dose given once weekly for 3 weeks. When kidney donors were Bf, ACI, or BN, rather than LBNF2, comparable effectiveness of continuous low dose treatment could not be demonstrated. Long survivors with renal allografts rejected donor skin normally with no adverse effect on the kidney. Serum from these animals in passive transfer experiments modified rejection minimally in normal rats when harvested shortly after drug treatment stopped. The mechanism of long-term graft survival in this drug treatment model is associated with normal cellular immunity, may transiently be related to specific serum factors, and ultimately may be attributable to graft adaptation or serologically undetectable enhancement.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYMice treated with antilymphocyte serum (ALS) at the time of placement of a firstset skin allograft were grafted additionally with second-set and third-party skin 2 weeks later. The survival of first-set grafts was consistently prolonged beyond the time of rejection of the second-set grafts. Such “adaptation” of first-set grafts was either diminished or abolished in presensitized mice or in mice given additional donor antigen in the form of splenic cells at the time of first-set grafting. Neither enhancing antibody nor residual leukocytes in the graft were thought to be significant factors in facilitating adaptation. The timing of immunosuppressive treatment with respect to the period of vascularization of the allograft by host endothelium may be an important determinant of adaptation.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYPeripheral blood lymphocytes from each of three multiply transfused patients exhibited cell-mediated immunity against freshly explanted allogeneic cells of known HL-A type. Lymphocytes from each donor damaged target cells obtained from siblings of identical HL-A genotype, as well as target cells obtained from unrelated persons of nonidentical HL-A type. Specificity of the reactions was demonstrated by the failure of immune lymphocytes to damage autologous cells. Target cell damage resulted from direct cell-mediated cytotoxicity independent of conventional complement-dependent antibody and other cell-free toxins. Cell-mediated cytotoxicity tests appear to detect membrane differences not detected by conventional HL-A typing.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

摘要:

SUMMARYGerm-free (BALB/c X C57BL/10)F1and BALB/c female mice and age-matched, conventional controls were challenged with mouse skin allografts of varying degrees of histoincompatibility: “strong” (H-2), “moderate” (non-H-2), and “weak” (H-Y) and with rat skin xenografts. No significant difference in survival times of first- or secondset grafts was found between the germ-free recipients and their conventional counterparts. Apparently, neither the absence of viable microorganisms nor the reduction of lymphoid tissue in germ-free mice has an effect on skin allograft and xenograft rejection.

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1972

数据来源: OVID