ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

Transplantation tolerance of the H-Y antigen may be induced and transferred by lymphoid cells, but not by nonlymphoid cells. In this study we have demonstrated that amongst lymphoid cells, T cells—but not B cells or macrophages—are capable of inducing transplantation tolerance. In contrast, all three populations express sufficient amounts of immunogenic H-Y antigen to sensitize adult female mice to a subsequent male skin graft, as demonstrated by the second-set reaction.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

The H-Y (male) antigen is detectable on 50% of eightcell mouse embryos. We show that 82% of the offspring resulting from the transfer of (C57BL/6XA/J)F1embryos surviving treatment with H-Y antibody and complement to the uteruses of pseudopregnant mice are female. The increased ratio of female-to-male births indicates that the H-Y-bearing embryos are male.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

The question is raised whether the H-Y transplantation antigen, as defined by skin graft rejection, is the same as the “male-specific” antigen that is identified by H-Y antibodies. We have addressed that question by injecting female mice of the inbred C57BL/6 strain with serologically defined H-Y antigen, and measuring the response of those female mice to skin grafts from intrastrain male mice. The results of our study indicate that the “two” male antigens are crossreactive or identical.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

Radiobinding assays using monoclonal H-Y antibodies were performed on human peripheral blood leukocytes, concanavalin-A-(conA)3stimulated blast cells, and fibroblast primary cell cultures. For peripheral blood leukocytes, male-specific binding was demonstrated only when the cells were enriched for T cells and the H-Y antisera were partially purified for the IgG or IgM fractions. Similar results were obtained with con-Astimulated blast cells. Fibroblast cells could be tested with unpurified H-Y antisera. With our monoclonal antibodies, best results were obtained using con A blast cells and fibroblasts.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

The minor transplantation antigen H-Y can cause graft rejection and can stimulate the generation of H-2-restricted T cell responses. We have used both responses to type karyotypically abnormal mice for the presence of H-Y antigen, in order to investigate the role of H-Y in sex determination. The mice under scrutiny wereSxr5-carrying females derived by crossing females carrying theT(16;X)16Htranslocation withSxrcarrying males. These females were fully fertile and were H-Y positive. These results are consistent with the testis determining gene,Tdy,which may or may not beH-Y,having a threshold effect on testis differentiation during embryogenesis. They also show that the presence of H-Y in adult females does not impair reproduction.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

Cytotoxic T lymphocytes (CTL)5of female origin that readily lysed syngeneic male lymphoid cells in specific, dose-dependent, H-2 restricted fashion had little or no activity against syngeneic male epidermal cells (EC) in short-term or long-term chromium-release assays. Moreover, although male EC were quite capable of priming syngeneic female lymphocytes in vivo for the accelerated rejection of male-specific skin grafts and for the subsequent generation of H-Y-specific CTL by exposure of primed female spleen cells (SC) to irradiated, syngeneic male SC in vitro, male EC themselves were incapable of stimulating the development of H-Y CTL when cocultured with primed female SC. Tests of EC from reciprocal male-female radiation chimeras revealed that keratinocytes, not marrow-derived EC (Langerhans cells), were reponsible for the priming ability of EC in vivo. Moreover, H-Y antigen was serologically defined on EC that failed to express H-Y-specific CTL target-cell determinants. Alternative explanations of these findings are discussed, including the possibility that the inability of H-2-restricted T cells to lyse male EC results from the lack of association of H-Y antigen and H-2 restricting elements on the EC membrane.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

Studies of the survival of weakly histoincompatible skin grafts in which the Langerhans' cells (LC)5have been replaced with major histocompatibility complex (MHC)-compatible or incompatible LC, as well as studies of the fate of cultured endocrine allografts in MHC-compatible and incompatible mice and rats, indicate that allografts are only recognized as foreign if they possess donor macrophages (or cells of this family), or if antigen presenting cells MHC-compatible with the graft can be provided by the host.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID

摘要:

The immunization of selected congenic strains and hybrids with the Ea-2.1 cellular alloantigen of the mouse shows that the anti-Ea-2.1 immune response is regulated by a gene or genes associated with the H-2 gene complex. It was earlier demonstrated thatH-2randH-2bwere, respectively, responder and nonresponder haplotypes. In this report it is shown that the haplotypes H-2dandH-2g2are also responder haplotypes, whileH-2a,H-2k,H-2m,H-2v,H-2q,H-2awlandH-2uare nonresponder haplotypes. The responderH-2g2and nonresponderH-2awlhaplotypes are recombinant configurations that map the Ea-2.1 regulator gene, or genes, within or adjacent to theI-Asubregion of theH-2 gene complex.

ISSN:0041-1337    

出版商:OVID    

年代:1984

数据来源: OVID