ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04096.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.During filtration leukapheresis a factor(s) is produced, released or extracted into rat blood which causes a transient granulocytosis in pheresed animals and in recipients of homologous plasma from these animals. To identify which factors contribute to this granulocytosis, the procedural steps involved in filtration leukapheresis as well as a number of chemical agents which are potentially extracted from of produced by the procedure, were tested for their ability to stimulate granulocytosis. Procedural steps tested included the depth of anesthesia, effect of the anticoagulant and possible interactions of blood cells with the plastic tubing in the system (sham‐pheresis). Chemical agents tested included common mediators of inflammation and proteinases released by polymorphonuclear leukocytes (PMNs), extracts of nylon fibers and Tygon tubing, nylon monomers and solvents used in the manufacture of nylon, oxidized and decomplemented plasma and lysates of PMNs or microorganisms. Our findings demonstrate that several of these agents contribute to the granulocytosis seen during filtration leukapheresis of rat

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04097.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.To evaluate the effective role of hepatitis viruses in thalassemic (Th) liver disease, we carried out a long‐term study in 42 subjects with nontransfusion‐dependent Th minor hospitalized for an episode of acute viral hepatitis. 10 patients had serologic evidence of hepatitis A, 23 of hepatitis B and 9 of hepatitis non‐A, non‐B. In the follow‐up chronic hepatitis was detected histologically in 5/23 patients with hepatitis B and 5/9 with hepatitis non‐A, non‐B. All hepatitis A patients recovered completely. The prevalence in 7 out of 10 patients with chronic hepatitis of piecemeal necrosis and of inflammatory changes over hepatic siderosis and fibrosis evidenced a determinant role of chronic viral infection in the development of liver damage in these patients. Thus, heterozygous nontransfusion‐dependent Th patients seem to have a high risk of developing a chronic inflammatory liver disease especially after an episode of non‐A, non‐B hepatitis. Therefore, in our geographical area, chronic hepatitis of viral origin should be taken into account, among other pathogenetic factors, in many cases of cryptogenic thalas

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04098.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.The incidence, etiology and risk factors of posttransfusion (PT) hepatitis were evaluated in a prospective study of 297 consecutive open‐heart surgery patients. PT hepatitis occurred in 63 (21.2%) patients with a significantly higher hepatitis attack rate in 51 recipients of commercial clotting factor concentrates (56.8%) compared to 246 recipients of blood units from single volunteer donors (13.8% p<0.001). Among the concentrates, Prothrombin‐complex showed the highest relative hepatitis risk (24) while in patients receiving only blood, the incidence PT hepatitis was correlated with the blood volume transfused. Of the 63 patients with PT hepatitis, 2 (3%) had hepatitis B, 8 (13%) showed evidence of cytomegalovirus infection and 53 (84%) had non‐A, non‐B hepatitis. These results show that in Italy, as elsewhere, non‐A, non‐B PT hepatitis is frequent, particularly when commercial blood produc

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04099.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.To maximize the availability of platelet concentrates (PC) and minimize their outdate, competing criteria, a computer simulation model of platelet production and distribution was developed. Based on 2 years of actual platelet orders placed with a regional blood center, the simulation program generated daily platelet orders, and also calculated mean demand and standard deviation of demand for each day of the week. The number of PC to be produced on a given day was calculated from: PC to be produced = (mean demand for that day of the week) +T× (standard deviation of demand for that day of the week) — (PC in inventory on the given day), where T is a selected multiple of the standard deviation. As T increases, availability is maximized, but outdating is expected to increase. Conversely, lower T is associated with less availability, but lower outdate. At the simulated platelet demand level (about 735 PC per week), with 3‐day platelet storage life, 99% availability is associated with 1% outdate and distribution of PC of less than 1 day average age. 100% availability, with no outdate is predicted for a 5‐day storage life, with no further improvement in logistics with 7‐day storage life, at this level of demand. Although logistics of platelet production and distribution vary from center to center, the simulation analysis is generally applicable, and a formal plan has the great advantage of predictive, rather than reactive, platelet pr

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04100.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.Platelet concentrates stored for 48 or 72 h were shipped up to 10 h at 22 or 4°C. Thereafter platelet function was compared with platelet concentrates stored under optimal blood bank conditions with continuous agitation and strict temperature control. Platelet function measured by hypotonic shock response, aggregation and serotonin uptake is better maintained at 22 than at 4°C. The only difference between shipping and continuous agitation is seen in the hypotonic shock response after a total storage time of 72 h. Shipped platelets showed a marked drop in hypotonic‐shock response. The data indicate that shipment of platelet concentrates does not significantly impair platelet funct

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04101.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Abstract.Spectrin‐free erythrocytic vesicles isolated from outdated liquid‐preserved blood samples show a distinct increase of the clot‐promoting activity compared with membrane phospholipid equivalents of intact erythrocytes. We suppose that, among other remodelling processes, local spectrin detachment from the inner membrane surface may trigger a flip‐flop‐mediated disturbance of the membrane lipid asymmetry. The interactions of spectrin with the cytoplasmic membrane surface seem to be essential for the structural membrane integrity including the lipid

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04102.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04103.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1983.tb04104.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY