摘要:

Abstract.The protein composition of human erythrocyte membranes was analyzed by discontinuous gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). A protein with a monomer mass of 95,000 daltons and the glycoprotein of highest monomer mass were both altered by extensive pronase digestion of whole erythrocytes. This confirmed that these two proteins were exposed to the external surface of the erythrocyte. Membranes prepared from fresh blood were compared with membranes prepared from blood stored for 3 weeks under blood bank conditions. In membranes from stored blood, a single major protein band, the surface protein of 95,000 daltons, was so extensively denatured that it was not monomerized by 0.1% SDS, and migrated much more slowly through the gel. When the membrane was dissolved in 1.0% SDS, this band was completely monomerized, and the electrophoresis pattern was indistinguishable from that produced by membranes prepared from fresh blood. Treatment with dithiothreitol or mercaptoethanol did not solubilize this band. Electrophoresis under the same conditions, followed by staining by the periodic acid‐Schiff base technique showed that the denaturable protein was not a glycoprotein, and that the surface glycoprotein was not affected by aging. This glycoprotein migrated in the same position when membranes from fresh and stored blood were compare

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02660.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.Human red cells, granulocytes and lymphocytes were examined electro‐phoretically. The effect of change of pH and ionic strength of the medium was determined. Lowering the ionic strength had the effect, not only of altering the electrophoretic mobility but also the characteristic shape of the pH mobility curves. Antilymphocyte globulin was shown to modify the mobility of granulocytes and lymphocytes, the latter to a greater extent. The results when compared to a silica surface completely covered with horse antihuman lymphocyte globulin show that the lymphocyte is only partially covered, suggesting a relatively low number of antigenic sites on the surfac

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02661.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.To try and identify those women at possible risk of Rh immunization during pregnancy and so limit ante‐partum treatment to them, 558 smears from 140 women were examined for fetal cells. Those found to have more than an estimated 0.05 ml of fetal blood were then given Rh IgG. Two of 134 untreated women and one of six treated women became immunized. The reason for the latter failure is uncertain. The total frequency of immunization, 2.2%, does not differ from that expected in the absence of treatmen

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02662.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A ‘new’ antibody that recognizes a high‐frequency red‐cell antigen in the Kell blood group system has been found in the serum of a K–k+, Kp(a–b+), Js(a–b+) person, who is heterozygous for the silentKogene. The antibody, named anti‐K13, reacted with all of 1,000 unselected blood samples, but does not react with the red cells of the propositus, one of his five siblings, nor with Kocells. Red cells of a child with chronic granulomatous disease and the McLeod phenotype were

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02663.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A new ‘private’ antigen was detected in a blood donor for our panel of test cells, Mme Hey. The original serum was derived from a blood donor sample during a routine screening test. The authors have tested 8,127 unrelated Caucasians and found two further Hey‐positive individuals. The antibody was looked for in 7,369 sera: three were found to react with the Hey red

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02664.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.In primary immunodeficiency diseases of childhood, antibodies reacting with human immunoglobulins and with bovine serum proteins were found predominantly in deficiency involving the IgA system. In generalized hypogammaglobulinaemia, no anti‐immunoglobulin activity could be detected. No clear‐cut correlation could be established between the clinical reactions to γ‐globulin injections, the appearance of anti‐IgA antibodies and previous transfusions or therapy with blood d

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02665.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A modification of the conventional antiglobulin test for routine use is described. Saline as the red cell suspension medium is substituted with salt solution of low ionic strength. This permits a considerable decrease in the incubation time, which is of practical importance in clinical work. The sensitivity of the reaction does not seem to be decreased by this procedure, in some instances even an increase in reaction strength can be observed.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02666.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A faggot made of glass microhaematocrit tubes coated with Formvar is made to fit inside a plastic disposable syringe. Granulocytes and monocytes, in a leucocyte suspension placed in this syringe, stick to the faggot, and a lymphocyte suspension can subsequently be run from the nozzle of the syringe.

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02667.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.A five‐bag system was developed and tested. It allows to wash red blood cells three times under sterile conditions starting from whole blood and to store them by addition of a PDG preservation solution at 4°C for 2 weeks.In vitroandin vivotests were performed. The system may be used also to wash and preserve prefrozen red blood cel

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02668.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY

摘要:

Abstract.Nineteen African tribes living in Uganda have been studied for ABO and Rh(D)antigens. High incidences of group A (41%), group B (30%), and group O (51%) were found in the tribes of West Nile, Busoga‐Bukedi, and Karamoja, respectively. Genetical mixing showing a southwestward ‘cline’ is apparent. The northern, eastern and central regions have less Rh(D)‐negative than the western region. The incidence of Rh(D)‐negatives increases from the north (less than 2%) to the south (about 6%).In the Kampala area, incidence is A 27%, B 20%, O 48%, AB 5%, and Rh(D)‐negative 2%. It is recommended that transfusion of blood without Rh(D)typing be discouraged. The practice would be more dangerous in the western regio

ISSN:0042-9007    

DOI:10.1111/j.1423-0410.1974.tb02669.x

出版商:Blackwell Publishing Ltd    

年代:1974

数据来源: WILEY