ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00098.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00099.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Serum iron and ferritin concentrations were measured in 1,532 regular blood donors from South Wales who were undergoing HLA typing prior to registration on the British Bone Marrow and Platelet Donor Panel. Serum transferrin concentrations were determined for donors with serum iron concentrations>24 µmol/1. There were 25 donors with transferrin saturations>50% and 11 with transferrin saturations>60%. There were five donors with serum ferritin concentrations>200 µg/1 (women) or>300 µg/1 (men). Two of the male donors had transferrin saturations>50% and serum ferritin>300 µg/1 on repeat blood samples and are being treated by venesection. Donors with HLA‐A3 did not differ from those without A3 in serum iron or ferritin concentrations. Even in the group of donors who were apparently homozygous for A3 there were neither abnormal serum iron nor ferritin concentrations.Although it is well established that measurements of transferrin saturation are required to detect homozygous haemochromatosis (HFE) in its earlier stages, the number of ‘false‐positive’ results is likely to be unacceptably high for screening blood donors. Serum ferritin assays should identify donors withHFEand iron overload before the onset of liver damage. With two million regular donors and 300,000 new donors each year, a significant proportion of the U.K. population will be screened within 10 years. The assay of serum ferritin identifies donors with low levels of storage iron who are at risk of developing iron‐deficiency anaemia. Furthermore, donation frequency may be increased for those donors with higher ferritin concentrations when blood sup

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00100.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary:An effective, modular automated blood grouping system has been developed which performs more effectively than the AG16C autogroupers and which uses readily obtainable reagents and disposables. Operating and maintenance costs are low by comparison with currently available equipment of similar capacity. The 99·2% success rate for blood grouping using this image analysis‐based system compares well with the 98·3% success rate on the AG16C autogroupers. Staffing requirements are reduced and the flexible modular approach allows this Newcastle system to be used for a range of serological functions, including grouping, antibody screening and screening for antigen donors. The ability to operate with small sample numbers without long start‐up times and the relatively low capital cost makes the Newcastle system an option that could be considered by the larger hospitals as well as transfusion centres. The sensitivity of the system could be developed to include compatibility testing as a feature of the s

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00101.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary:The new low‐frequency antigen STEM was identified when a Cape coloured woman with an unknown antibody in her plasma (which agglutinated the red cells of her husband and a minority of other individuals) gave birth to a baby suffering mildly from haemolytic disease of the new‐born. Most, but not all, examples of anti‐STEM distinguish different strengths of STEM antigen on the red cells of different people; the different strengths are inherited. Family studies established that STEM was inherited as a Mendelian dominant character. STEM subdivides hrs––– (Rh: ––– 18, ––– 19) and hrB––– (Rh: ––– 31, ––– 34) red cells into two types: STEM + and STEM –––. The manually calculated lod score for STEM being associated with the Rh system is 3·91 and LIPED calculated lod score 4·35. The International Society of Bloo

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00102.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary:Poor stability of plasma factor VIII in whole blood and loss of erythrocyte 2,3‐bisphosphoglycerate (BPG) during red cell storage are limitations with systems for blood component preparation in current use. This study presents attempts to improve post‐collection storage conditions in both these respects using half‐strength citrate CPD solution (0·5CPD) for blood collection, which has been shown by others to improve the stability of factor VIII, and some compositions of hypotonic additive solutions for red cell storage containing citrate, adenine, mannitol, and phosphate. Guanosine was also included in some of the media. The erythrocyte BPG concentration was maintained at a normal level for 3–4 weeks with the best of the tested compositions. Total adenine nucleotide concentration was maintained at the original level for 49 days and adenosine triphosphate for 28 days. Spontaneous storage haemolysis was low, 0·31% (mean) ± 0·08‐0·10% (SD) after 49 days in the two best compositions. The intracellular pH was 0·2‐0·3 pH units higher than the extracellular pH at the beginning of storage, but this difference gradually diminished and disappeared after 4–5 weeks. We suggest two likely explanations of the effects: the maintenance of intracellular pH at a level sufficiently high not to impair BPG synthesis until after several weeks of storage, and a sufficient supply of phosphate needed in the synthesis of organic phosphate compounds. The content of citrate was selected such that the total amount supplied to a patient in a massive transfusion, when using a combination of 0·5CPD plasma and red cell suspension, would be smaller than that provided by a transfusi

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00103.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary:The von Willebrand factor (vWf) activity, as measured by the ristocetin co‐factor (vWf:RCo) and collagen binding (vWf:CBA) assays, declined progressively in standard blood units stored at 4°C after a 2‐day storage period. This loss of activity was accompanied by a loss and degradation of high molecular weight (HMW) vWf multimers. In studies using a paired design, filtration of blood with a high efficiency leucocyte‐removal filter, prior to storage at 4°C, led to significantly improved maintenance of vWf:RCo and vWf:CBA compared with unfiltered units (P,<0·01 after 8 days). Loss and degradation of HMW vWf decreased when blood was filtered prior to 4°C storage. Filtration had no effect on vWf‐associated activities when blood was stored at 22°C for 10 days.These results indicate that part of the storage lesion of vWf in banked blood is due to leucocyte‐mediated removal and degradation of HMW vWf. This has implications when specifying plasma for the production of vWF concentrates and may also play a role in the haemostatic lesion associated with massive transfusion o

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00104.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary:In order to evaluate the influence of intensity of plasmapheresis on donor serum total protein and immunoglobulin concentrations, these parameters were measured monthly in two groups of plasma donors over a period of 6 months. Donors in group one donated 500–600 ml of plasma at weekly intervals and those in the other groups at intervals of 14 days or longer. Regular whole blood donors were used as a control group. The average concentrations of total protein and IgG immunoglobulin fraction in group one were significantly lower (P<0·002) than those of the other two groups but they always remained well within the normal ranges. Although the mean total protein level of this group of donors fell significantly during the first 3 months, their values returned to almost baseline levels at the end of the study. No statistically significant difference from the initial concentrations was observed during monthly measurements of IgG, IgA and IgM levels among any of the groups studied. We conclude that removal of 500–600 ml of plasma at weekly intervals involves little, if any, risk of total protein or immunoglobulin depletion in donors who satisfy current criteria. This study also suggests that the frequency of IgG and IgM evaluations may be safely lowered and that IgA determinations may be limited to first time plasma do

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00105.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary:The reaction pattern of monoclonal anti‐D with category DIIcells differed from those of other category D cells. DIIcells express epDl, epD2, epD3, epD5, epD6/7 and epD8 but lack epD4 and a new epitope epD9. The new epitope, epD9, is proposed to explain the failure of some monoclonal anti‐D (previously considered to be anti‐epD3) to react with DII

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00106.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A1 INFLUENCE OF POSTURE ON REACTIONS IN NEW BLOOD DONORS.A2 A CONFIDENTIAL UNIT EXCLUSION SYSTEM IDENTIFIES DONORS WITH A POTENTIAL FOR HIV INFECTION.A3 A STABLE BLOOD SUPPLY FOR THE FUTURE: THE RECRUITMENT OF 16 TO 18 YEAR OLD DONORS TODAY AND THEI CONTRIBUTION AS COMMITTED REGULAR DONORS OF TOMORROW.A4 APPROACH TO A SUPPLY CRISIS OF HYPERIMMUNE RHESUS PLASMA FOR THE PRODUCTION OF RhD IMMUNOGLOBULINA5 THE INFLUENCE OF AGE, SEX AND ABO BLOOD GROUP ON THE INCIDENCE OF CMV ANTIBODIES IN SYDNEY BLOOD DONORS.A6 THE INCIDENCE OF CATEGORY VI AMONGST WEAK Rh(D) POSITIVE SYDNEY BLOOD DONORS.A7 A MODIFIED METHOD FOR DETECTING HIGH TITRE ANTI‐A AND ANTI‐B IN GROUP O DONORSA8 IMPROVING THE CLINICAL SPECIFICITY OF ALANINE AMINO TRANSFERASE (ALT) TEST RESULTS WITHIN THE NORMAL BLOOD DONOR POPULATION OF QUEENSLAND.A9 EXTRACTION OF HCV RNA USING A GUANIDINE ISOTHIOCYNATE METHOD.A10 HEPATITIS C VIRUS (HCV) ANTIBODY DETECTION IN TASMANIAN BLOOD DONORS.A11 EFFECTIVE INTERNAL QUALITY CONTROL FOR ENZYME IMMUNOASSAYSA12 DETECTION OF ANTIBODY TO NON‐PATHOGENIC RETROVIRUSES (SPUMAVIRUSES) IN HUMAN SERUMA13 DETECTION OF ANTIBODY TO NON‐PATHOGENIC RETROVIRUSES (SPUMAVIRUSES) IN HUMAN SERUMA14 A NOVEL BLOOD BAG SYSTEM WITH POTENTIAL, FOR THE ASIA‐PACIFIC MARKET.A15 DESIGN OF CONTAINERS SUITABLE FOR THE TRANSPORT OF RED CELL, PLATELET AND FROZEN PLASMA PRODUCTS.A16 EVALUATION OF INDICATOR LABELS FOR QUALITY ASSURANCE OF IRRADIATION PROCEDURE OF BLOOD PRODUCTS.A17 MOLECULAR TYPING FOR UNUSUAL ABO TYPES.A18 AN EXAMPLE OF THE RARE ABO SUBGROUP,A19 RFLP ANALYSIS OF A RH NULL BLOOD DONOR.A20 A RELATIONSHIP BETWEEN LEWIS ERYTHROCYTE PHENOTYPES AND COLORECTAL CANCER.A21 PATERNITY TESTING USING SINGLE LOCUS DNA PROBES: OBSERVATIONS ON THE REFERENCE DATA BASE SIZEA22 USE OF FAMILY AND POPULATION STUDIES TO DETERMINE THE SPECIFICITY AND INHERITANCE OF NEUTROPHIL ANTIGENS DEFINED BY PLANT LECTINS.A23 SAMPLING PLANS: IS THERE RELEVANCE FOR BLOOD COMPONENT QC?A24 QUALITY MANAGEMENT: HOW DO WE DO IT IN A STATE THE SIZE OF QUEENSLAND?A26 THE ENERGY METABOLISM OF CIRCULATING CELLS.A27 ACETATE UTILISATION RATES AND THE EFFECT OF GLUCOSE‐FREE PLASMA IN PLATELET CONCENTRATE STORED IN A MIMIMAL MEDIUM (MPM).A28 IMPROVED LEVELS OF 2,3 DJPHOSPHOGLYCERATE IN RED CELL SUSPENSIONS PREPARED FROM BLOOD COLLECTED INTO DEXTROSE‐FREE ANTICOAGULANT.A29 EVALUATION OF RED CELL FREEZING METHODS AS A PRELUDE TO ADOPTING ‐80° C FREEZING IN HIGH GLYCEROL IN ROUTINE PRACTICE.A30 CLUMPING IN PLATELET CONCENTRATES ‐ AN UNSOLVED PROBLEM.A31 AUTOLOGOUS BLOOD: SAFE FOR OTHERS OR NOT?A32 ESTABLISHMENT OF AN AUSTRALIAN HAEMOPHILIA TREATMENT CENTRE DATA BANK.A33 EXPERIENCE IN THE USE OF ROBOTICS AND MICROPLATE TECHNOLOGY TO SEMI‐AUTOMATE A ROUTINE HOSPITAL BLOOD BANK.A34 AN ANTI‐IgAl/IgA2 ELISA ASSAY FOR THE INVESTIGATION OF HYPESENSITIVITY TRANSFUSION REACTIONS.A35 THE INFLUENCE OF IgG AGGREGATES AND FRESH NORMAL SERUM ON THE MONOCYTE MONOLAYER ASSAYA36 DETECTION OF Rh(D) POSITIVE FETAL CELLS IN PREGNANT Rh(D)‐NEGATIVE WOMEN BY FLOW CYTOMETRY.A38 HAEMOSTAT‐IX: A HIGH PURITY FACTOR CONCENTRATE FOR THE TREATMENT OF PATIENTS WITH HAEMOPHILIA B.A39 GRAVITY FILTRATION OF PLASMA FROM DONOR BLOOD UTILISING A HOLLOW FIBRE FILTER MEMBRANE DEVICEA40 The Therapeutic Device Problem Reporting Scheme, and the Victorian Red Cross Blood BankA43 HIGH FREQUENCY ANTIBODIES AND THE ADVANTAGES OF MANUAL POLYBRENE.A44 FACTS AND FANTASY IN THE DEVELOPMENT OF PLATELET ADDITIVE SOLUTIONS.A45 LACK OF EFFECT OF STORAGE CONTAINER ON STORAGE OF PLATELETS PREPARED FROM DEXTROSE‐FREE BLOOD,A46 PLATELETS PREPARED FROM DEXTROSE‐FREE BLOOD MAY BE STORED WITHOUT AGITATION.A47 QUALITY OF BED CELL CONCENTRATE IN HOSPITALS COMPARED TO THE BLOOD BANKA48FLOW CYTOMETRIC CHARACTERISATION OF LEUCOCYTE ‐ DEPLETED RED CELL CONCEHTRATES.A49 PRODUCTION AND CHARACTERISATION OF HUMAN MONOCLONAL ANTI‐D ANTIBODIES.A50 CD55 AND CD59 SUSCEPTIBILITY TO PROTEASE TREATMENT AND THE RESULTANT EFFECT ON COMPLEMENT LYSIS OF RBCs.A51 DIRECT COMPARISON BETWEEN PLATELET STORAGE CONTAINERS ‐ IMPROVEMENT IN STORAGE CHARACTERISTICS OF TUTA PLATELET BAGS OVER THE PAST FOUR YEARS.A52 IMPROVED SOLID‐PHASE MIXED PASSIVE HAENAGGLUTININ ASSAY (MPHA) WITH FROZEN PANEL PLATELETS FOR THE DETECTION OF HUMAN PLATELET ANTIBODIES.A53 DEVELOPMENT OF A SOLVENT DETERGENT TREATED THROMBIN CONCENTRATE AS A COMPONENT OF A FIBRIN GLUE KIT.A54 Autologous blood transfusion: a promotional programmeA55 AVAILABILITY OF BLOOD PRODUCTS FOR ACUTELY BLEEDING PATIENTS.A56 REMINISCENCES OF 50 YEARS A. A TRANSFUSION ST.A57 A NATIONAL SYSTEM FOR REPORTING TRANSFUSION REACTIONS TO FRACTIONATED BLOOD PRODUCTS.A58 EFFECT OF FLUORESCENT LIGHTING ON THE VISUAL APPEARANCE OF PLATELET CONCENTRATESA59 USING A MICROWAVE OVEN TO THAW FRESH FROZEN PLASMA.A60 COAGULATION CAPACITY OF POOLED PLATELET PLASMA.A61 A COMPARISON OF IMMUNOHAEMATOLOGY SURVEY PERFORMANCE BETWEEN NEK ZEALAND AND AUSTRALIAA62 COMPATIBILITY TESTING: ARE ENZYME TESTS REQUIRED?A63 AN EVALUATION OF THE DIAMED MEROTYPING SYSTEM FOR THE PERFORMANCE OF THE DIRKT ANTIGLOBUDIN TEST.A64 ASSESSMENT OF PERFORMANCE IN BLOOD GROUP ANTIBODY DETECTION.A65 CHARACTERISATION OF MABS TO THROMBIN‐HIRUDIN COMPLEXES WITH IMMUNOASSAY POTENTIAL.A66 MONITORING ANTT‐HPA‐la (P1A1) PLATELET ANTIBODY LEVELS DURING PREGNANCY USING THE MAIPA TEST.A67 COMPARISON OF PIFT AND MAIPA TEST“ IN THE DETECTION OF ANTI‐HPA‐la (PIA1) PLATELET ANTIBODIES.A68 USE OF PLATELET‐CROSSMATCHING IN SUPPORT OF A CASE OF MYELODYSPLASIA WTTH A PLATELET SPECIFIC AND B LYMPHOCYTE ANTIBODYA69 The Pattern of Leucocyte Antibody formation in Transfused Patients.A70 DETECTION OF HPA‐Ia ANTIBODY IN BREAST MILKA71 ANALYSIS OF PRENATAL SCREENING.A72 DETECTION OF MINOR POPULATIONS OF ERYTHROCYTESA73 MODIFICATIONS TO THE MCNOCYTE‐MEDIATED ADCC ASSAY.A74 AN AUTO ANTI‐JMH; GAMMA‐CLONE POLYSPECIFIC AHG AS A USEFUL TOOL.A75 CLINICALLY SIGNIFICANT ANTI‐A1 DERIVED FROM B LYMPHOCYTES FOLLOWING SINGLE LUNG TRANSPLANTATION.A76 CONFIRMATION THAT ANTI‐ELO CAUSES HAEMOLYTIC DISEASE OFTHE NEWBORN.A77 ANTI‐DoaSTIMULATED BY PREGNANCY.A78 DONOR IgM ANTI‐A ASSOCIATED WITH HAEMOLYTIC TRANSFUSION REACTIONA79 COLLECTION OF GRANULOCYTES AND PLATELETS USING FENWALL CS 3000 AND HAEMONETICS 30 CELL SEPARA

ISSN:0958-7578    

DOI:10.1111/j.1365-3148.1993.tb00107.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY