摘要:

15 patients with aplastic anemia were prospectively followed after having measurements of myeloid progenitor cells in bone marrow and blood. Treatment included androgens, low or high dose steroids and standardized supportive care. The median length of survival was 5.8 months. When patients were grouped according to the numbers of myeloid progenitor cells present in their blood and bone marrow, we found that the survival length of aplastic patients with higher progenitor cell numbers was prolonged when compared to that of patients with lower numbers of these cells. The prognostic information obtained from such in vitro cultures was particularly indicative when the patients were grouped according to ‘growth’ and ‘no growth’: the absence of colony-forming myeloid stem cells was associated with survival times significantly shorter than those of patients whose cells maintained their colony-forming capacity. Besides initial numbers of myeloid progenitor cells, only initial numbers of granulocytes were related to survival length. Thus, the measurement of myeloid progenitor cells in bone marrow and blood can be of prognostic value in patients with aplastic

ISSN:0001-5792    

DOI:10.1159/000206263

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

In a first small study the chlorinated hydrocarbons present in the bone marrow of haematologically normal persons and of 6 randomly selected patients with severe aplastic anaemia have been identified and quantified by glass-capillary gas chromatography. We found great interindividual differences in the concentrations of these compounds both within the control group and within the patient group, but no definite difference between the two groups. So far, we have been unable to detect known toxic concentrations of chlorinated hydrocarbons in any of our samples, which could have supported the idea of a deficiency in the metabolism of these chemicals in some patients with aplastic anaemia.

ISSN:0001-5792    

DOI:10.1159/000206264

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

78 previously untreated patients (aged 16–82 years) with acute nonlymphoblastic leukemia received intensive chemotherapy for induction of remission. In 64 patients, the combination used consisted of daunomycin and cytosine arabinoside with or without 6-thioguanine. The overall remission rate was 59 and 87% for those who received what was considered as adequate therapy. Patients who achieved remission received maintenance and, in part, late intensification. The median duration of remission was 9 months. The median survival of the patients who attained remission was 24 months, compared to 15 months for the entire patient group and 3 months for patients who failed to achieve remission. Age had no effect on the rate and duration of the remission obtained but the median survival was significantly longer in patients below the age of 50 year

ISSN:0001-5792    

DOI:10.1159/000206265

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

The hemoglobin content of single erythrocytes was determined by microspectrophotometry as total extinction (TE) at 415 nm in subjects with a wide spectrum of hemoglobin levels and erythrocyte indices. Precise measurements of the cellular area (A) and the ratio TE/A were also recorded. A significant correlation was found between TE and mean corpuscular hemoglobin (MCH), A and mean corpuscular volume, and TE/A and mean cell hemoglobin concentration (MCHC) for the cases studied. In addition, TE, A and TE/A were determined in fetal erythrocytes obtained by fetoscopy at the 20th week of gestation for prenatal diagnosis of β-thalassemia. The mean red cell TE and TE/A of the group of fetuses diagnosed to have thalassemia major were significantly lower than those of the group of normal controls and with heterozygous β-thalassemia. The significant differences of TE, A and TE/A between maternal and fetal blood allowed a safe distinction of the latter. The calculated MCH of the fetuses with thalassemia major was 12% less than that of the normal controls; the respective difference of the mean cell size was 5 % and that of MCHC 3 % lower than norma

ISSN:0001-5792    

DOI:10.1159/000206266

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Four possible methods of preparing young red cells (YRBC) which exploit the relationship between red cell ageing and density are compared. Of these, two cell separators and the IBM cell washer are shown to be capable of producing YRBC suitable for clinical use. YRBC prepared from the IBM 2991 cell washer are recommended for clinical trials because of lower cost, greater convenience and lower white cell contamination compared to cell separator-derived YRBC.

ISSN:0001-5792    

DOI:10.1159/000206267

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

The hypothesis that human red cells contain activity of ribulose-1,5-diphosphate (Ru-1,5-P2) oxygenase, an enzyme that catalyzes the synthesis of phosphoglycolate, was examined. The putative Ru-1,5-P2 oxygenase was partially purified from human red cells using DE-52 chromatography and (NH4)2SO4 fractionation by monitoring Ru-1,5-P2-dependent 3-phosphoglycerate formation. The synthesis of [32P]phosphoglycolate from [1-32P]Ru-1,5-P2 was attempted in the presence of the partially purified preparation of the provisional Ru-1,5-P2 oxygenase. There was no formation of radioactive phosphoglycolate even under 100% oxygen gas, indicating the absence of this enzyme activity in human red cells. Together with our previous report that glycolate kinase in human red cells is not responsible for the synthesis of phosphoglycolate in vivo, these studies raise the questions whether there is actually phosphoglycolate in red cells as well as whether novel pathways for its synthesis exist.

ISSN:0001-5792    

DOI:10.1159/000206268

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

A family with a new factor X defect is reported. The proposita is a 56-year-old female. She is asymptomatic and no consanguinity is present between the parents. The main features of the defect are: prolongation of prothrombin time and derivative tests but normal partial thromboplastin time. Factor X was found to be low (about 25–30% of normal) only if tissue thromboplastins were used in the assay system. Chromogenic substrate S-2222 also yielded decreased factor X levels. However, factor X activity was normal with cephalin and cephalin-RVV mixture. Factor X antigen was normal in three immunological systems (electroimmunoassay, an Elisa method and laser nephelometry). Crossed immunoelectrophoresis and antigen-antibody kinetics recorded in a laser nephelometer failed to show major differences from normal factor X. Both sons of the proposita, the father and other family members showed slightly decreased factor X levels and normal factor X antigen and were considered heterozygous for the abnormality. The toponym factor X Padua is proposed to indicate this peculiar abnormalit

ISSN:0001-5792    

DOI:10.1159/000206269

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Angio-immunoblastic lymphadenopathy is a systemic disease of unknown aetiology. It carries a high mortality mainly from infection which results both from the intrinsic immunodeficiency and its exacerbation by treatment with steroids and or cytotoxics. We report a case of angio-immunoblastic lymphadenopathy with the unusual feature of hypogammaglobulinaemia who died of miliary tuberculosis. We suggest that all patients with angio-immunoblastic lymphadenopathy who have inactive tuberculous foci should be given antituberculous prophylaxis.

ISSN:0001-5792    

DOI:10.1159/000206270

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

2 cases of diffuse large-cell non-Hodgkin’s lymphoma developing in conjunction with idiopathic myelofibrosis are described. In neither case could any predisposition to neoplastic transformation be discerned. The possible pathogenetic implications of this association are discussed, and a brief literature review of the relationship between myeloproliferative and lymphoproliferative disease is presente

ISSN:0001-5792    

DOI:10.1159/000206271

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

A unique association of a transient IgM λ monoclonal gammopathy and a serum sickness-like illness, appearing after a short course of indomethacin, is described. The IgM λ monoclone did cryoprecipitate along with fibronectin in hypotonic medium and disappeared with the resolution of the clinical picture. Emphasis is given to the role of some foreign antigens in the induction of benign monoclone

ISSN:0001-5792    

DOI:10.1159/000206272

出版商:S. Karger AG    

年代:1985

数据来源: Karger