ISSN:0001-5792    

DOI:10.1159/000207140

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

The direct immunoperoxidase technique was employed to show the localization of transferrin in immature human erythroid cells. The present method is interesting in that use was made of a small marker (HRP-Fab’ complex) and in that the endogenous peroxidase was blocked under a controlled condition – this ensured good structural preservation allowing electron-microscopic examination. Cytoplasmic transferrin was found not only in the micropinocytotic vesicles but also within the mitochondrial membrane. Similar findings were made in all the immature erythroid cells examined, each of which was in a different erythropoietic condition. Thus, it may be concluded that the present study provides morphological evidence for the endocytotic iron transport to the mitochond

ISSN:0001-5792    

DOI:10.1159/000207141

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

A case of severe anemia complicated with lactic acidosis (blood lactic acid concentration 17.5 mmol/l) is presented. Circulatory failure and other causes of lactic acid accumulation could be excluded. Transfusion treatment resulted in a rapid correction of the metabolic derangements. Activation of anaerobic metabolism is prevented in anemia by the highly efficient compensating mechanisms of the organism. Only an extreme fall of the blood hemoglobin concentration below 2 g/dl may result in lactic acidosis.

ISSN:0001-5792    

DOI:10.1159/000207142

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

Circulating immune complexes in sera from sickle cell anemia (SCA) patients were investigated using inhibition of complement-dependent lymphocyte rosette formation (EAC rosette inhibition) and the anticomplementary assay. A significant inhibition of EAC rosette formation was seen in seven (77%) sera from SCA patients with hepatic crisis. Four (44%) sera from these patients showed slightly increased anticomplementary activity. These preliminary results provide evidence for the presence of circulating immune complexes in some SCA patients with hepatic crisis.

ISSN:0001-5792    

DOI:10.1159/000207143

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

IgG, IgA and IgM levels were studied in 187 homozygous β-thalassemic patients and compared with age-matched normal control subjects. The not yet transfused and the polytransfused nonsplenectomized patients showed a significant increase of all Ig classes. The polytransfused splenectomized patients showed a significant increase only of IgG and IgA. The splenectomized patients, when compared to the nonsplenectomized ones, showed a significant increase of IgG, of IgA in the elder ones and a significant reduction of IgM. High ASLO and ASFLO titers were found especially in the splenectomized patients. The high Ig levels in younger not yet transfused patients, with little iron storage and normal hepatic enzyme values, demonstrate that transfusion therapy and liver damage do not play a main role in hypergammaglobulinemia. The absence of antimitochondrial, anticonnective tissue and homogeneous antinuclear antibodies seems to exclude a deficient suppressor mechanism. The hemocatheteric ‘overworking’ of RES may reduce the antigen clearance with a consequent increase of antibody response. Splenectomy, by removing a consistent part of RES, enhances the phenom

ISSN:0001-5792    

DOI:10.1159/000207144

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

A case of Hb H disease from Algeria was studied at the genetic and molecular level in order to delineate the pattern of α-thalassemia in the Mediterranean population. The family study indicated that both parents had the hematological and clinical manifestation of α-thalassemia trait and that the affected sibling had homozygous α-thalassemia with 5.6% Hb H, microcytosis and an α-/non-α-biosynthetic ratio of 0.64. Hybridization in globin cDNAα excess suggested that the molecular defect responsible for this form of α-thalassemia is a partial deletion of the haploid stock of α-globin genes. The Algerian case of Hb H disease studied thus differs from Asian and Negro cases by the mode of inheritance of the α-thalassemia mutation

ISSN:0001-5792    

DOI:10.1159/000207145

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

The quantitative and/or qualitative distribution of liver iron was assessed in 81 transfusion-dependent thalassemia major patients with chronic liver disease (36 with chronic active hepatitis, 23 with chronic persistent hepatitis, 22 with siderosis). Viral marker studies showed only 3 cases with both HBsAg and anti-HBc posiüvity in the serum, while the others had anti-HBc and anti-HBs or only anti-HBs or no B viral markers. A significantly higher iron overload was found in chronic hepatitis, particularly chronic active hepatitis, than in siderosis. This increased iron overload may be due to less intensive chelation treatment, higher intestinal absorption secondary to lower mean Hb levels, and/or to liver inflammation-depedent iron deposition. The liver iron overload in turn may facilitate the development or persistence of chronic progressive liver disease

ISSN:0001-5792    

DOI:10.1159/000207146

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

Percentage of megathrombocytes, platelet regeneration time (PRT) and platelet-Associated IgG (Pl-A-IgG) were investigated in 12 patients with clinical features consistent with idiopathic thrombocytopenic purpura and in 11 patients with thrombocytopenia associated with chronic liver disease. Bone marrow smears were also examined and megakaryocytes classified into stages I–III according to the current principle. Of 12 patients with idiopathic thrombocytopenic purpura the percentage of megathrombocytes was increased in 9, PRT reduced in 10, and Pl-A-IgG increased in 8 patients. A statistically significant correlation was found between the percentage of megathrombocytes and the level of Pl-A-IgG. A slight correlation was also found between PRT and the percentage of megathrombocytes, while a significant correlation was found between megakaryocytes in stage I and the percentage of megathrombocytes, suggesting that growth of megakaryocytes probably contributes to platelet heterogeneity. In patients with thrombocytopenia and chronic liver disease, the percentage of megathrombocytes was in the normal range. A moderately increased level of Pl-A-IgG was found only in patients with active chronic hepatitis, PRT was reduced only in a few patients, while most of them also showed an increased level of Pl-A-Ig

ISSN:0001-5792    

DOI:10.1159/000207147

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

95 cerebrospinal fluid samples from leukemic children, 50 with and 45 without meningeal relapse, were studied for glucose, total protein, protein electrophoresis, immunoglobulins, lactic dehydrogenase and its isoenzymes, muramidase and leukocyte count, in addition to blast cell investigation. Only total protein and albumin were elevated in children with CNS leukemia, this increase persisted for at least 2 weeks after intrathecal treatment began and was independent of presence of blast cells. Our findings also indicate that protein elevation has no value as prognostic factor for future CNS infiltrations.

ISSN:0001-5792    

DOI:10.1159/000207148

出版商:S. Karger AG    

年代:1981

数据来源: Karger

摘要:

Primary Hodgkin’s disease of the thyroid is rare since only 13 cases have been described. This report documents a case in a 57-year-old woman with a painful goiter. She was treated by subtotal thyroidectomy. The patient still remains symptom-free 6 years after the initial diagnosis. This fairly good prognosis is in accordance with the evolution of the 13 other cases reported in the literature. The role of immunolocalization of intracellular immunoglobulin in the differential diagnosis of primary lymphoma and undifferentiated carcinoma of the thyroid is discusse

ISSN:0001-5792    

DOI:10.1159/000207149

出版商:S. Karger AG    

年代:1981

数据来源: Karger