ISSN:0001-5792    

DOI:10.1159/000207016

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The conditions, accuracy, precision and possible errors of the thrombin time assay of plasma fibrinogen are determined. Comparison with an estimation of clottable protein by absorbance at 280 nm gave a correlation coefficient of 0.96 and the regression line y = 1.00 × + 0.56 (n = 34). Comparison with a radial immunodiffusion method yielded the correlation coefficient 0.97 and the regression line y = 1.18 × = 2.47 (n = 26). The presence of heparin in clinically applied concentrations produced a slight shortening of the clotting times. The resulting error in the estimated concentrations of fibrinogen was too small to affect the clinical usefulness of the determinations. The influence of fibrin(ogen) degradation products was significant only in excessive amounts in samples containing low levels of fibrinoge

ISSN:0001-5792    

DOI:10.1159/000207017

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The plasma from a patient with thrombotic thrombocytopenic purpura contained a low molecular weight dialysable factor which inhibited the synthesis and release or activity of prostacyclin-like activity from vascular tissue. This factor was not an immunoglobulin or complement component. Following fresh plasma infusions the ability of the patient’s plasma to stimulate the release of prostacyclin-like activity returned but no clinical improvement occurre

ISSN:0001-5792    

DOI:10.1159/000207018

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

In the literature the values for phospholipid content and the pattern of human gel-filtered platelets diverge considerably. In order to find the possible reasons for these discrepancies, the separation of plasma constituents from gel-filtered platelets and the influence of different extraction methods were reinvestigated. The critical point in phospholipid determination of platelets appeared to be the completeness of plasma lipid separation from platelets and not the extraction method. Avoiding contamination by plasma lipids the results with gel-filtered platelets correlated to that obtained with washed platelets.

ISSN:0001-5792    

DOI:10.1159/000207019

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Phagocytosis of the heat-killed opsonised yeast, Candida guilliermondii, by human neutrophils resulted in the rapid release of a potent factor which suppressed granulopoiesis in vitro. The factor has been shown to act on monocytes and macrophages by inhibiting the production and release of colony-stimulating factor (CSF) which is the specific stimulator of granulocytic colony-forming cell (CFUc) proliferation in vitro. When added directly to target cells containing CFUc, the inhibitory factor had no effect on colony growth. The absence of detectable inhibitor in media conditioned for short periods with resting neutrophils or neutrophils challenged with unopsonised Candida which are not phagocytosed confirmed that active phagocytosis was the stimulus for inhibitor release. In further experiments, addition of endotoxin to the cultures was shown to suppress the inhibitory effect. We suggest that feedback inhibition of CSF production in vivo may be mediated by products derived from phagocytosing neutrophils.

ISSN:0001-5792    

DOI:10.1159/000207020

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Peripheral blood cells from untransfused rabbits that were treated with a moderate dose of benzene during 2 months were cocultivated with autologous and normal bone marrow. In a significant number of experiments, CFUE and BFUE growth of both autologous and normal bone marrow was suppressed. Further experiments showed that this inhibition was associated with adherent mononuclear peripheral blood cells. It is concluded that benzene, an established myelotoxic agent, also induces CFUE and BFUE inhibiting activity in adherent peripheral blood cells.

ISSN:0001-5792    

DOI:10.1159/000207021

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Progenitor cell fractions from normal human marrow have been prepared by a method that enables low numbers of cells to be cultured and results in very little T cell or monocyte contamination. The enrichment of 14- and 21-day BFU-E was similar to that for CFU-GM and the readdition of sheep red blood rosette-forming cells had no effect on BFU-E growth. The significance of the discrepancy in cellular requirements for the growth of blood and bone marrow BFU-E is discussed.

ISSN:0001-5792    

DOI:10.1159/000207022

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The presence of iron on the transferrin molecule increases its affinity for and sojourn time on the reticulocyte. This could be due to selective internalization of iron-containing transferrin molecules. This possibility was investigated by electron microscopic autoradiography. Rabbit reticulocytes were incubated with rabbit transferrin at 6, 33, and 72% iron saturations, and the distribution of transferrin molecules at membrane and intra-cellular locations was assessed by grain counting. The results showed that (1) both apo-transferrin and iron transferrin enter the cell interior and (2) the amount of intracellular transferrin was primarily controlled by the concentration of membrane-bound transferrin and not by its iron saturation.

ISSN:0001-5792    

DOI:10.1159/000207023

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

In this study maximum urinary iron elimination with continuous desferrioxamine subcutaneous infusion was obtained in thalassemia major patients with chronic persistent or active hepatitis with lower doses (60 mg/kg) than those necessary in patients without hepatitis (80 mg/kg). Since dose-response curves were highly variable the treatment schedule should be tailored to the individual needs of each patient. Both groups may achieve iron balance but chronic hepatitis patients have more frequently a net urinary iron excretion. In patients with chronic hepatitis no correlation was found between serum ferritin levels or serum ferritin/aspartate aminotransferase ratios and transfusional iron overload while serum ferritin/aspartate aminotransferase ratios were seen to be correlated with liver iron stores.

ISSN:0001-5792    

DOI:10.1159/000207024

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Four homozygotes for β-thalassemia with normal hemoglobins A2 and F were studied. The absence or scarcity of transfusion requirement and comparatively low hemoglobin F content were the most important findings. Both parents of 3 patients showed the findings of β -thalassemia with normal hemoglobins A2 and F. Biosynthetic studies in 2 patients and their both parents showed moderate or mild β -chain deficiency. The possible reason for this comparatively mild course of a β -thalassemia syndrome lies in a mild deficit in β -chain produc

ISSN:0001-5792    

DOI:10.1159/000207025

出版商:S. Karger AG    

年代:1982

数据来源: Karger