摘要:

Megakaryocytopoiesis in polycythemia vera (PV) was characterized by in vitro growth of marrow megakaryocytic progenitors (CFU-Meg) and quantitation of megakaryocyte numbers in marrow biopsy specimens in 14 patients with PV. Megakaryocyte numbers and CFU-Meg numbers in the 14 patients were variable, with values from the control range to markedly increased numbers. Nine of the 14 patients showed spontaneous CFU-Meg growth, and the presence or absence of spontaneous CFU-Meg growth was found to be related to the degree of marrow megakaryocyte increase, i.e., those with spontaneous CFU-Meg had higher megakaryocyte numbers. None of the plasmas from the PV patients contained detectable levels of megakaryocyte colony-stimulating activity (Meg-CSA), assayed using the culture with nonadherent normal marrow cells and tested plasma without phytohemagglutinin-stimulated leukocyte-conditioned medium as an exogenous source of Meg-CSA. Increased megakaryocyte numbers and resultant thrombocytosis in PV are likely based on the abnormal population of CFU-Meg.

ISSN:0001-5792    

DOI:10.1159/000205681

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Five patients with hepatitis-associated aplastic anemia were transplanted with HLA-identical, mixed lymphocyte culture-compatible sibling marrow. One patient who had suffered from severe chronic graft-versus-host disease died from intracranial bleeding at 42 months following the transplant. The other four patients are surviving from 15 to 54 months after transplant with a median follow-up of 21 months. Previous hepatic damage from viral hepatitis and liver function abnormalities existing at the time of grafting do not appear to increase the risk of hepatic venocclusive disease or the side effects of ciclosporin in patients with hepatitis-associated aplastic anemia.

ISSN:0001-5792    

DOI:10.1159/000205682

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Ferrokinetic and RBC mass determinations were made at 3-month intervals in iron-replete hemodialysis patients randomized to a control group or to nandrolone decanoate therapy. After 3 months, RBC mass increased in two of 4 androgen-treated patients. Erythron iron turnover, an index of RBC production, increased in the one responder studied but not in the two nonresponders. Similarly, in a fifth subject, who was not restudied until 6 months of androgen therapy were completed, an increase in RBC mass was associated with an increase in erythron iron turnover. However, between 3 and 6 months, RBC mass increased in all 4 androgen-treated patients studied even though erythron iron turnovers remained unchanged and dialysis-associated blood losses did not decrease. Thus, at least two androgen-treated patients had increases in RBC mass without ever increasing their erythron iron turnover. Two of three control subjects also had increased erythron iron turnovers, which in one case was related to increased dialysis-associated blood losses. Changes in RBC mass were not consistently paralleled by changes in Hb. These findings suggest that increases in RBC mass during nandrolone decanoate therapy result from two mechanisms: increased erythropoiesis (shown by simultaneous increases in RBC mass and erythron iron turnover) and increased RBC survival (indirectly shown by increases in RBC mass without increases in erythron iron turnover). The importance of control groups, RBC mass determinations and the monitoring of dialysis-associated blood losses in studying the effects of androgens on erythropoiesis in chronic hemodialysis patients is also demonstrated.

ISSN:0001-5792    

DOI:10.1159/000205683

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Twelve patients with clinical and laboratory findings typical of essential mixed cryoglobulinemia, type II (EMC II) underwent multiple liver and bone marrow biopsies. In 9 of 12 cases (all hepatitis B surface antibody-negative), routine histology revealed patent infiltration of liver portal tracts, lobules and sinusoids by small lymphocytes provided with cytological characteristics closely resembling those of the LP immunocytoma of the Kiel classification. At immunophenotyping on frozen sections, these elements expressed the CD22 antigen (marker of B cells) and bore the same type of immunoglobulin (IgM/k = 8, IgM/λ = 1) as the monotypic component in the serum. Furthermore, in 7 of 9 patients repeated bone marrow needle biopsies showed multiple foci of infiltration by plasmacytoid cells, often with paratrabecular location. In the remaining 3 cases (all hepatitis B surface and core antigen-positive), liver biopsies were consistent with a diagnosis of cirrhosis (two) or chronic active hepatitis (one). In two of them, however, Jamshidi needle biopsy evidenced bone marrow infiltrates quite similar to those observed in the other group. On the basis of these findings, the authors discuss the hypothesis that most EMC II are substained by a low-grade malignant lymphoma

ISSN:0001-5792    

DOI:10.1159/000205684

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

The phenotype and functions of monocytes in patients with haemophilia A and age-matched controls were studied. Fourteen male haemophiliacs were classified in three categories according to the mean number of units of factor VIII received during the last 5 years. Eleven patients were positive for antibodies to human immunodeficiency virus but none of our patients were homosexuals or drug abusers, nor do they fulfill the criteria of acquired immunodeficiency syndrome. Patients treated with high amounts of factor VIII concentrates ( > 3 3× 105 U/year) showed a significantly lower percentage of monocytes expressing HLA-DR, LFA-1 and CR3 antigens as compared with patients receiving lower amounts of factor VIII ( < 2 × 106 U/year) or controls. Kinetics of DR, LFA-1 and CR3 in cultured monocytes showed tht they were lost faster by monocytes from haemophiliacs treated with large amounts of factor VIII than by control monocytes. Adherence ability and chemotactic response of monocytes from patients treated with < 3 × 105 U/year of factor VIII were also impaired. Although phagocytic indices were in normal ranges in haemophiliacs, a significant difference was observed between percentages of phagocytic monocytes from haemophiliacs treated with the largest doses of factor VIII and normal controls. Tests for respiratory burst activity, measured by chemiluminescence and superoxide anion generation, and Staphylococcus aureus killing were in normal ranges in haemophiliacs’ monoc

ISSN:0001-5792    

DOI:10.1159/000205685

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Primary polycythaemia (PP), idiopathic myelofibrosis (MF), essential thrombocythaemia (ET) and chronic granulocytic or myeloid leukaemia (CGL) are clonal disorders of the pluripotent haemopoietic stem cells. We have studied granulocyte, megakaryocyte and erythroid progenitors from the peripheral blood of 7 patients with PP, 9 with ET, 19 with MF and 6 with CGL in order to characterise similarities and differences at the committed progenitor cell level. Spontaneous megakaryocytic and erythrocytic growth was characteristic of MF, PP and ET but was not seen in CGL. Circulating erythroid (BFU-E) and granulocyte/macrophage (CFU-GM) progenitors were markedly increased in MF and CGL, less raised in ET and closest to normal in PP. Erythropoietin-independent erythroid bursts (EIBFU-E) grew from the blood of patients with MF, PP and ET but spontaneous growth of megakaryocytes occurred in only MF and ET. These results suggest a progression of increasing abnormality from PP, where EIBFU-E occurred with relatively normal numbers of circulating progenitors, to ET where both EIBFU-E and megakaryocyte precursors regularly occur with elevated numbers of progenitors, to MF where spontaneous BFU-E, CFU-Mk and CFU-GM occur at high levels.

ISSN:0001-5792    

DOI:10.1159/000205686

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

The phagocyte function of granulocytes and monocytes of whole blood was measured as superoxide production in patients treated for acute nonlymphoblastic leukaemia. The results indicate that the antileukaemic protocol used in this study did not cause a decrase of the oxidative metabolism triggered by phagocytosis and by phorbol esters.

ISSN:0001-5792    

DOI:10.1159/000205687

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

The effect of verapamil on the surface ultrastructure and calcium content of human platelets was studied by scanning electron microscopy and X-ray microanalysis. The results showed that the drug prevents platelet pseudopodia formation and causes a dose-dependent inhibition of platelet calcium concentration. The significance of these findings in the explanation of the inhibitory effect of verapamil on platelet aggregation is discussed.

ISSN:0001-5792    

DOI:10.1159/000205688

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

The plasma cortisol levels of 108 children aged 1–16 years with various Hb genotypes (AA, AS and SS) were measured. The mean plasma cortisol levels of both the AS and SS groups (during steady state) fell within the normal range but the mean of the SS group is significantly lower than the AA group. In fact, 9 of the sickle cell children had plasma cortisol levels below the normal range while no child in the HbAS and HbAA groups had plasma cortisol below the normal range. These results indicate that in sickle cell disease, there is low cortisol production while the secretion is increased during painful crisi

ISSN:0001-5792    

DOI:10.1159/000205689

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Acquired Pelger-Huet anomaly has been found in association with both hematologic and nonhematologic diseases. While its association with myeloid hematologic disorders is well known, this granulocytic anomaly has also been found in chronic lymphocytic leukemia, multiple myeloma and Hodgkin’s disease. This report describes a case of acquired Pelger-Huet anomaly in non-Hodgkin’s lymphoma and reviews the association of this anomaly with both lymphoid and myeloid hematologic disord

ISSN:0001-5792    

DOI:10.1159/000205690

出版商:S. Karger AG    

年代:1988

数据来源: Karger