ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

The authors studied five patients with primary cutaneous Hodgkin's disease (PCHD). Each patient presented with skin lesions without evidence of systemic HD. Skin lesions were papules or nodules, many of which regressed spontaneously. Lesions were distinguished from lymphomatoid papulosis (LyP) by the presence of numerous diagnostic Reed-Sternberg (RS) cells that expressed CD30 and CD15 but were negative for CD45R; LyP lesions usually are CD15 -, CD45R+. Anaplastic large cell lymphoma (ALCL) was excluded by the polymorphous background of inflammatory cells in PCHD. Three patients with PCHD had a benign course without systemic disease with up to 20 years of follow-up, whereas two other patients developed mixed-cellularity HD in lymph nodes 2 months and 6 years following the onset of PCHD. This study indicates that PCHD does occur as a rare but distinct clinicopathologic entity morphologically and immunophenotypically indistinguishable from nodal HD but with an unexpectedly indolent course in some patients. Patients with PCHD should be observed for development of systemic HD, but unlike patients with LyP or ALCL, an association of PCHD with mycosis fungoides or cutaneous T-cell lymphoma has not yer been observed.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

Spitz nevus is a well-known histologic simulator of malignant melanoma. Vascularity of human neoplasms has been associated with prognosis as well as propensity for metastasis. Were vascularity significantly different between melanoma and Spitz nevus, it could serve as a feature to distinguish between these two neoplasma. We evaluated the vascularity of a series of primary cutaneous melanomas ranging from thin superficial spreading to nodular lesions as well as superficial compound and nodular deep Spitz nevi by using light-microscopic evaluation of factor-VIII-stained sections. Vascularity was density graded and microvessels were counted per X200 and X400 field. Vascular density and microvessel counts were significantly different between melanomas of 2+ and 3+ vascularity compared with all types of Spitz nevi. Although nodular malignant melanomata tended to have a greater degree of vascularity than thin melanomas, there was a significant population of both of these subtypes having few or many vessels. We conclude that the number of microvessels per X200 and X400 field in areas of greatest vascularity is significantly less in Spitz nevus than in malignant melanoma. There is a subset of melanoma, however, in which the number of microvessels may be low. Nodular melanomata tended to have a greater number of vessels than did thin superficial spreading lesions. Evaluation of microvessel count may be of assistant when coupled with clinical and histologic findings in distinguishing between melanoma and Spitz nevus in selected cases.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

Using a panel of seven cell markers, we studied the value of immunocytochemical labelling in the histological diagnosis of desmoplastic malignant melanoma. Sections from routine formalin-fixed tissue of 45 surgical specimens were obtained from 25 cases of malignant melanoma that showed well-marked desmoplastic or neurotropic features. Routinely stained sections (Haematoxylin-and -eosin and melanin stains) were compared with the following panel of seven antibodies: S-100, neuron-specific enolase (NSE), vimentin, factor XIIIa (FXIIIa), desmin and the newer, supposedly more specific anti-melanoma antibodies HMB45 and NKIC3. S-100 and NSE were the most sensitive antibodies for desmoplastic malignant melanoma with strong labelling of spindle cells in most cases. In contrast, results for NKIC3 were more variable; results were negative in nearly half the tumours, but strong labelling was seen in six cases (27%). Positive labelling for HMB45 was noted in five tumours (22%); it was mostly confined to small groups of cells in the superficial part of the lesions. Tumour spindle cells were negative for FXIIIa in all cases; there was no increase in the number of positive dermal dendritic cells compared to conventional and spindle cell melanoma. All tumours were desmin-negative, but most were vimentin-positive. Our findings indicate that immunocytochemistry is of less value in the diagnosis of desmoplastic malignant melanoma that it is with other types of malignant melanoma. However, positive or negative labelling for S-100 protein and NSE is useful for suggesting or excluding a diagnosis of desmoplastic malignant melanoma; neither marker is specific and, in particular, positive labelling is also found in most neurofibromas and benign cellular naevi. NKIC3 and HMB45 label desmoplastic malignant melanoma much less consistently than other conventional malignant melanomas.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

Apoeccrine glands were found in 13 of 57 specimens of nevus sebaceus. The glands showed the basic structure of eccrine glands, but differed in that a part of the secretory duct was lined by characteristic apocrine epithelium. The apocrine alteration varied from slight apocrine changes in a few secretory epithelium in other glands. The association with changes in the excretory ducts of rare glands suggested a transformation of eccrine into apocrine glands. It was nevertheless considered as most likely that a majority of the apocrine glands in nevus sebaceus had developed in the “classic” way in association with pilosebaceus structures. In nevus sebaceus, apoeccrine glands appeared to represent eccrine glands with a variable degree of apocrine metaplasia that could result in complete transformation rather than the formation of a separate category of specialized sweat glands. The capacity of eccrine epithelium to transform into apocrine epithelium is why difficulties are experienced in the precise categorization of sweat gland neoplasma into eccrine and apocrine tumors.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

To demonstrate elastic tissue and its reactions in the skin and other organs, the hematoxylin-eosin staining method had been modified in two ways: (a) the hematoxylin solution is used once only to obtain its full potential, and (b) aqueous eosin is given greater selectivity by controlling its pH with a buffer. This strain highlights the broad supporting role of elastic tissue, particularly in pressure-containing blood vessels. It also helps to define the hyperplasia, degeneration, and elastolysis that arise from its peculiar sensitivity to actinic radiation.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

Cutaneous neurofibromas consist of heterogenous cell populations including Schwann cells, perineurial cells, and fibroblastlike cells. However, the histogenesis of neurofibromas, particularly the origin and nature of the fibroblastlike cells, is still controversial. Recently, cells containing blood coagulation factor XIIIa have been reported in cutaneous neurofibromas, although their identity is uncertain. In this report, by the combination of double immunohistochemical straining and immunoelectron microscopy, we demonstrate that factor-XIIIa-positive cells are distinct from Schwann cells, perineurial cells, endothelial cells, mast cells, and conventional macrophages, but correspond to the fibroblastlike cells in cutaneous neurofibromas. Such factor-XIIIa-positive cells in cutaneous neurofibromas, however, differ from conventional fibroblasts in the strong expression of HLA-DR antigen and lack of prolyl 4-hydroxylase. Similarly, so-called endoneurial fibroblasts and, occasionally, connective tissue cells within perineurium and epineurium in normal peripheral nerve fibers express factor XIIIa as well as HLA-DR antigen. The results suggest that fibro-blastlike cells in cutaneous neurofibromas are probably derived from factor-XIIIa- and HLA-DR antigen-positive connective tissue cells in peripheral nerves. The role of such factor-XIIIa-positive cells in the growth and development of cutaneous neurofibromas is discussed.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

In situ hybridization is an important tool in molecular and developmental biology to detect specific nucleic acid sequences (either mRNA or DNA) within cells. This technique is especially applicable to tissue sections since it provides information about the spatial distribution of DNA or mRNA sequences. However, previous studies utilizing in situ hybridization in the skin were hampered by a high degree of nonspecific background, which has made interpretation of the results difficult. In this paper, we demonstrate how refinements in in situ hybridization techniques, combined with laser-scanning confocal microscopy, significantly reduce nonspecific background and produce improved resolution of in situ hybridization in skin specimens. The sensitive detection method of laser-scanning confocal microscopy allows three-dimensional localization of S35radioactive-labeled riboprobes within the emulsion of specimens, which is not possible with conventional bright or dark field light microscopy.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID

摘要:

Follicular mycosis fungoides is a rare variant of cutaneout T-cell lymphoma. We report a patient with thin plaques of mycosis fungoides studded with follicular papules. Histologically, these lesions were composed of dense infiltrates of lymphocytes within and around follicular epithelium (“folliculotropism”). This case indicates that follicular mycosis fungoides is a distinct clinicopathologic entity. It is different from follicular mucinosis associated with mycosis fungoides. Conceptually, the relationship between follicular mycosis fungoides and ordinary mycosis fungoides is analogous to the relationship of lichen planopilaris to ordinary lichen planus.

ISSN:0193-1091    

出版商:OVID    

年代:1994

数据来源: OVID