ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02827.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02828.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02829.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Experiments are described for T‐independent polyclonal activation of B cells in vitro by the immunoglobulin binding substance (IBS) from the granary weevil. The affinity‐chromatographically purified IBS was used. IBS is a heat‐, alkali‐ and acid‐stable glucopeptide which is characterized by non‐specific immunoglobulin binding lo the Fab fragment. The purified IBS consists of three polymer homologues whose molecular weights are 12–14,000, 25–30,000 and more than 150,000 Da. IBS did not stimulate DNA synthesis by murine T cells, macrophages or plasma cells whereas it did stimulate that by mature B cells without any help from T cells or macrophages, IBS also stimulated both in vitro IgG production by spleen cells and in vitro sensitization of spleen cells by sheep red cells (SRBC). IBS was found to stimulate DNA synthesis by B cells mediated by binding to surface immunoglobulins of B cells. IBS is thought to be a useful amplifier for inducing human hybridomas and a valuable tool for examining mature B cells, both diagnostically and

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02830.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Peripheral blood lymphocytes from two polytransfused renal dialysis patients were transformed by Epstein Barr virus, fused to heteromyeloma and cloned. Eight human monoclonal antibodies from the resulting clones were tested for their binding to variety of antigens by ELISA, indirect immunofluorescence and immunoblotting. Antigens tested included B‐cell lines, T and B lymphocytes, red blood cells, chronic lymphocytic leukaemic B cells, IgG, ssDNA, dsDNA. histones, nucleoprotamine, sperm nuclei, thymus and spleen extracts. MOLT4 cell lysatcs, affinity purified autoantigens, tetanus toxoid, bacterial lipopolysaccharide, insulin, and tissue section screen. These human monoclonal antibodies reacted with more than one antigen to varying degrees and were autoreactive and pulyreactive. One of these heterohybridoma cell lines exhibited cytoplasmic staining with an anti‐CD5 monoclonal. Our findings support the concept that in adult individuals subset of B cells produce heterogeneous IgM antibodies which can bind to variety of different autoantigens and also to foreign antigens. These monoclonals were different from the autoantibodies usually seen in renal dialysis patients in the sense that they were not lymphocytoto

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02831.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Ig‐producing immunocytes and epithelial expression of secretory component (SC) and HLA‐DR were evaluated by two‐colour immunofluorescence staining in 10 normal and 20 inflamed salivary glands; the latter included specimens from 10 patients with obstructive sialadenitis and 10 with Sjögren's syndrome (SS), Epithelium adjacent to T‐cell infiltrates showed extensive co‐expression of SC and HLA‐DR, suggesting that leucocyte‐derived cytokines were responsible for this concurrent up‐regulation. Clusters (>2 positive cells) of IgA‐producing cells were spatially related to DR‐positive ducts. The possibility is discussed that DR‐expressing epithelium contributes to local terminal differentiation of IgA‐producing plasma cells. A cytokine‐mediated up‐regulation of SC that simultaneously increases the transport capacity for polymeric IgA would constitute an efficient enhancement of secretory immunity

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02832.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The circulatory pool of B cells from the majority (11/13) of chronic hepatitis B surface antigen (HBsAg) carriers contained sensitized B cells with the capacity to secrete IgG antibodies with specificity for human serum albumin (HSA), when stimulated withE, coli‐derived core protein at low concentrations in vitro. The IgG anti‐USA secretion was dependent upon and regulated by T cells, and optimal secretion was obtained at T/B‐cell ratios of 1.0–4.0, varying for different individuals. The level of anti‐HSA secretion was higher for patients with on‐going viral replication as assessed by hepatitis B virus (HBV)‐DNA in serum. Culture supernatants containing anti‐HSA antibodies also contained anti‐HBc antibodies, as detected by enzyme‐linked immunosorbent assay (ELISA) where the solid phase was charged withE. coli‐derived core protein, or the synthetic peptides corresponding to the 75–84 and 132–147 sequences in the C region of HBV, In contrast, IgG anti‐HBc (E. coli‐derived), but no anti‐MSA or anti‐HBc 75–84, 132–147 antibodies, were detected at similar T/B‐cell ratios in cell cultures from 56 individuals with naturally acquired immunity to hepatitis B. These data indicate that peripheral B cells from the majority of HB‐immune donors are sensitized lo unique (e.g. non‐albumin associated) structures in the nucleocapsid of HBV, while B cells in the majority of chronic HBsAg carriers are sensitized to linear C‐gene‐derived structures in

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02833.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The elution pattern obtained when amyloid fibrils from amyloid‐laden bovine kidneys were subjected to gel filtration under dissociating conditions revealed a larger amount of non‐AA material (eluting between the void volume and protein AA) than usually seen in other species. SDS PAOF, of this non‐AA fraction yielded several Coomassie blue stained bands. The most distinctive ones gave estimated molecular masses of 15 kDa, 18 kDa, 33 kDa and 43 kDa. These molecular species were electroblotted onto PVDK membranes, and wore further characterized by amino acid composition analyses, cyanogen bromide cleavage and N‐terminal analyses. The results revealed that the intermediate “non‐AA” fraction consisted of histones H2B, H3 and H4 in addition to protein AA also found in

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02834.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

In this study we report on functional characteristics of pustule as well as blood polymorphonuclear neutrophils (PMN) in patient suffering from relapsing bullous staphyloderma. Large numbers of viable PMN from newly formed pustules as well as from the peripheral blood were investigated. During the course of disease chemotactic migration, enzyme degranulation, superoxide‐anion generation and leukotriene B4production were determined simultaneously.The results revealed C5a‐ and NAP‐1/IL‐8‐specific dysfunction of pustule PMN as compared with blood PMN. In contrast, FMLP‐elicited functional activities of pustule PMN were only slightly affected.Our findings provide evidence that in inflamed tissue invading PMN are regulated by in situ generated mediators. C5a produced by staph. aureus‐induced activation of the alternative pathway of the complement cascade represents predominant regulatory factor in situ. Furthermore, the results substantiate previous observations concerning different modulation of C5a and f‐met‐peptide recep

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02835.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Secretory immunoglobulins A (SIgM) and M (SIgM)were investigated in 20 sera containing high levels of monoclonal polymeric IgM or IgA. In the sera of patients suffering from Waldenström's macroglobulinemia (WM), the level of SIgA was found to be low, whereas that of SIgM was extremely high. Reciprocally, in the multiple myeloma (MM) patients, SIgA were increased and SIgM were dramatically decreased. Electrophoretic analysis showed these SIgA and SIgM to have the same monoclonal pattern as the corresponding paraprotein. Hence these molecules must originate from the malignant clone. The most likely mechanism involved is an intravascular formation of the secrelory‐like immunoglobulins. Free secretory component (SC) could diffuse passively from the digestive lumen and bind the circulating myeloma polymeric immunoglobulins. Such a possibility of in vivo binding of free SC to IgM and IgA polymers leads to a reconsideration of the secretory origin of SIgM and SIgA in normal human ser

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1992.tb02836.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY