摘要:

By means of immunofluorescence technique, lymphocytes with surface‐bound immunoglobulin were detected in the blood of normal humans F(ab')2, IgM, IgG, IgA, the four IgG subclasses, and the Gm markers Gm(f) and Gm(Z) were demonstrated on cells. IgM was the dominating class, and IgG2 the dominating IgG subclass. The data suggest that the Ig on any one cell belongs to a single class and IgG subclass. Very few lymphocytes stained for antigens on the C‐terminal half of ihe IgG molecule. Ig‐positive lymphocytes were also demonstrated in cord blood and in spleen cell suspensions. An almost total lack of Ig‐positive lymphocytes was demonstrated in the blood of 8 patients with severe hypogammaglobulinemia. The Ig‐positive lymphocytes are thought to be analogous to B‐lymphocytes in ot

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03730.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Ninety macroglobulinaemia sera were tested for occurrence of multiple monoclonal immunoglobulins. Thirteen (14%) of the sera contained more than one monoclonal immunoglobulin. The homogeneous macroglobulins were isolated from 3 sera; in each case individually specific antigenic determinants common to the two components were demonstrated, in addition to determinants unique for each component. NH2‐terminal amino acid sequence studies in two patients showed that the IgM heterogeneity was due to differences in the primary structure of the variable parts of the μ (case Tö) and the μ (case æ) chains. Macroglobulinaemia appears to be a unique source of homogeneous immunoglobulins from related c

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03731.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Ninety macroglobulinaemia sera were tested for occurrence of multiple mono clonal immunoglobulins. Thirteen (14%) of the sera contained more than one monoclonal immunoglobulin. The homogeneous macroglobulins were isolated from 3 sera; in each case individually specific antigenic determinants common to the two components were demonstrated, in addition to determinants unique for each component. NH2‐terminal amino acid sequence studies in two pa tients showed that the IgM heterogeneity was due to differences in the primary structure of the variable parts of the x (case To) and the μ (case Nae) chains. Macroglobulinaemia appears to be a unique source of homogeneous immuno‐globulins from related cl

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.00019.pp.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The protective effect of antiserum toE. colibacteria was studied in intraperitoneally infected mice. It was found that protection was obtained with O and K antibodies. There was no indication that H antibodies could prevent infection. 19S (IgM) and 78 (IgG) antibodies showed about the same protection against infection.

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03732.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

We coupled bacteriophage T4 with standard (0.16%) or low (0.005%) concentrations of NIP‐azide. The low concentration produced haptenated phage (type B) that was inactivated by the IgG anti‐NIP of some rabbits but not by that of other rabbits similarly immunized. The most active IgG fraction at an anti‐NIP concentration of 100 μg/ml inactivated the type B phage with a titre of 2400, while the same anti‐NIP concentration of the least active IgG fraction from another rabbit had a titre of 0.3. With the standard haptenated phage titres only varied within a 20‐fold range. IgM fractions of a few rabbits tested did not appear to exhibit a great variation with the type B phage. The IgG fractions with high titres against the type B phage bound more NIP‐hapten at low (∼ 10−10M) hapten concentrations than those with a low titre, but this superiority was greatly reduced by an increase of hapten concentration to 10−8M. The same phenomenon could be demonstrated by an inhibition technique, lnactivation of type B phage appeared to detect exclusively antibodies with association constants considerably higher than 108. Its affinity threshold seems to be higher than the threshold of two other methods employed, haemagglutination of haptenated erythrocytes and inactivation of the standar

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03733.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Interaction of mouse immunoglobulins with a mast cell tumor HC was studied by the rosette technique. All mast cells formed rosettes, and this could be inhibited by IgG1myeloma proteins, less efficiently by IgG2, and not by IgA or IgM. Antigen antibody complexes formed under appropriate con ditions also inhibit, with at least 100‐fold greater efficiency than free im munoglobulin. Antisera containing reaginic activity also inhibit rosettes in the presence of antigen, but on heating the sera, a concomitant loss of reaginic activity and rosette inhibition occurs. It is proposed that there is a specific receptor on the mast cell surface for IgG1and reaginic immunoglobulins, and that binding to this site in the form of a complex of correct antigen ratio, will initiate the anaphylactic proces

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03734.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Subfragments of the pFc fragment (equivalent to the Cγ3 homology region) of human IgG have been obtained by tryptic and chymotryptic digestion. Tryptic digestion for 4 or 8 hours removed N‐terminal peptides of 5 and 7 amino acid residues respectively. Chymotryptic digestion appeared to remove an N‐terminal pentapeptide and also approximately 14 residues from the C‐terminus. However, chymotryptic cleavage in the presence of trypsin inhibi tor removed the C‐terminal peptide and only 2 N‐terminal residues. Haemag‐glutination‐inhibition tests for Gm activity indicated that both of the tryptic fragments retained Gm(a), (x), (b°), and ‘non a’ activities. In contrast, the chymotryptic fragments lacked Gm(a), (x), and ‘non a’ activities, but retained Gm (b°) activity. These results support our earlier hypothesis that certain C‐terminal amino acids of the 7‐chain are essential for the serological expres sion

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03735.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

A mixture of rabbit IgM anti‐human A1red cell antibody and a human125I‐labelled monoclonal IgM was reduced with mercaptoethanot and exposed to oxygen under conditions in which most of the half subunits coalesced to IgM subunits with reformation of interchain disulphide bonds. Hybrid molecules were demonstrated in the mixture by precipitin tests with autoradiography and by binding of125l to human A1red cells. By careful selection of IgM antibodies with high energy in each binding site, such hybrids may be useful ‘IgM coats’ for haemagglutination inhibition tests attempting to define sub‐class‐specific and genetically determined anti

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03736.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

It has been found possible to separate cells according to their surface anti gens by the use of antibody‐coated columns. High efficiency columns were made by double‐layer principles, first coating beads with antigen followed by antibody in excess. Such columns could be shown to contain a high amount of free antigen‐binding sites for the relevant antigens. Lymphoid cells were thus fractionated according to their surface concentration of immunoglobu lin and a highly selective retention of mouse B lymphocytes was observed when filtering spleen cells through an anti‐immunoglobulin column prepared according to the above procedure. No evidence of retention of mouse T lymphoid cells was observed in the same system. By the use of anti‐gamma‐2a immunoglobulin columns, it was found possible to deplete a population from memory cells potentially capable of synthesizing gamma‐2a antibodies. No evidence was found that columns prepared in the described manner would function through combining with receptors on lymphoid cells for antibody‐antigen complexes. By using anti‐A blood group columns, it was possible to selectively retain cells (erythrocytes or kidney cells) with A blood group anti gen on their surface. High‐avidity immune antibodies were found to be more efficient than ‘natural’ anti‐A antibodies in this test. No evidence was found of anti‐A antibodies being adsorbed on to the passing cells as tested by in vitro serological tests and tissue culture experiments. The applications of a technique for separating cells according to their surface antig

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03737.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Allogeneic mouse spleen cells of strong hlstocompatibility difference mixed in vitro at equal proportions are unable to make a primary antibody response against thymus‐dependent as well as thymus‐independent antigens. This non‐responsiveness is not due to mutual cytotoxicity, because the reactivity of the cell partners can be recovered if the mixtures are transferred to irradiated hosts isoimmune to one of the cell partners. Responsiveness of allogeneic cell mixtures depends on the proportions at which the allogeneic cells are mixed, the strength of histocompatibility differences, the functional state of the ad mixed cells, the timing of the admixture, and the T/B cell ratio of the ad mixed cells. Supernatants from cultured allogeneic spleen cell mixtures can restore responsiveness of otherwise non‐responsive B‐spleen cells. The results suggest that interaction between cultured allogeneic T‐cells leads to rapid formation of soluble material which, depending on concentration, can either initiate or inhibit the maturation of B‐cells into antibody‐secreting cells in the presence of s

ISSN:0300-9475    

DOI:10.1111/j.1365-3083.1972.tb03738.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY