摘要:

Abstract –A method is presented by which rat facial processes from different stages were obtained in pure fraction. The morphology, and protein and DNA contents in free dissected facial processes were determined. Facial processes of embryonic rats aged 9‐15 days were analyzed by isoelectric focusing for their isoenzymic distribution of four enzymes: lactate dehydrogcnase, creatine phosphokinase, fructose diphosphate aldolase and phosphoglycerate mutase. A dominance of LDH‐5, LDH‐4 and LDH‐3 isoenzymes was observed. As a comparison, LDH isoenzymes from mandibular and maxillary processes of rat embryos aged 9‐11 days only revealed LDH‐5 and to a smaller extent LDH‐4. The results support the presence of a prominent anaerobic metabolism in these tissues during early facial development. The change to LDH‐3 development correlates well with the formation of new blood vessels. From the ninth embryonic day, isoenzyme BB of creatine phosphokinase was present and during days 10‐15 MB and MM developed. Isoenzyme As4of fructose diphosphate aldolase was present from the ninth embryonic day and isoenzymes A3C and A2C2developed during days 10‐15. From the tenth embryonic day, isoenzyme BB of phosphoglycerate mutase was present and during days 10‐15 isoenzyme MB and MM developed. Isoenzyme development was first seen in mandibular processes, followed by maxillary, lateral nasal and medial nasal processes, and it preceded morphologic evidence of sk

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01357.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –Fluoride given as a high single dose to young rats with developing molars has earlier been shown to cause subameloblastic cysts with disorganized ameloblasts in the cystic wall and an irregular mineralization pattern of the underlying enamel. In the present study irnmunohist‐ochemistry has been employed to determine if an increased permeability of the enamel organ occurred at the areas of cell disturbances. For this purpose 5‐day‐old rats were injected with 60 mg sodium fluoride per kg body weight. They were decapitated after 24 h, the maxilla prepared histologically and paraffin sections incubated for the demonstration of IgG according to the avidin‐biotin‐peroxidase complex method. Staining for IgG was present in the cystic lumina and in areas of disorganized ameloblasts. No reaction was observed in areas of unaffected ameloblasts. It was suggested that the fluoride‐induced cell injury increased the permeability of the ameloblastic cell layer. Diffusion of IgG and most likely other substances as well, through the ameloblastic layer may have contributed to cyst formation and to the irregular mineral deposits that have been found in the ameloblastic layer and at the e

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01358.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –Clinical tissue changes were recorded during restorative treatment of proximal carious lesions of young premolars and molars. 60 proximal surfaces with radiolucencies in the inner half of the enamel or the outer half of the dentin were treated. The extent and character of the tissue changes were documented with photographs taken during drilling and evaluated after magnification. The maximum extent for each lesion was correlated to the extent of the radiographically observed lesion. The results showed that 70% of the restored surfaces were associated with a breakdown of the enamel surface. When the radiolucency did not extend deeper than into the inner half of the enamel, cavities were found in 61%; for lesions with a radiolucency extending into the outer half of the dentin the percentage was 78%. In the majority of cases the cavity was limited to the enamel. In all cases discoloration was observed in the enamel. The dentin was soft and discolored in 83%. Severe damage of tooth substance was found in 12% of the teet

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01359.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –A non‐destructive, in vivo method of measuring the enamel hardness directly on patients is described. Normal values on 39 students arc given and compared with those of five patients, one of whom suffered from abnormal oral acid concentration, and the others from the effects of various degrees of hyposialosis caused by radiologic treatment of tumors in head and neck. The difference between the two groups was significant below the 0.1% level. It was suggested that the method may be used to test the degree of salivary gland inactivation after radiologic treatment and the efficiency of different saliva substitutes used to alleviate the effects of hyposialo

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01360.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –Anti‐keratin staining patterns were examined by immunofluorescence in the epidermis, oral mucosa and forestomach of rats and mice by monoclonal antibodies AE1 and AE2. In contrast to human tissues enzymatic pretreatment of sections was necessary even when freshfrozen tissue was used, suggesting masking of the antigens in vivo. AE1 stained the cytoplasm of spinous cells in most epithelia, whereas basal cell staining varied. AE2 showed suprabasal cytoplasmic staining in epidermis, forestomach and palate, whereas the other epithelia were stained only in keratohyalin granules and membranes of cornified cells. In some epithelia a small number of irregularly distributed basal cells stained positive with AE2, indicating heterogeneity in the basal cell compartment. Thus, the anti‐keratin staining pallern varies both regionally and, as earlier shown in human epithelium, with the stage of maturation of cells within a given epithelium. The present study provides a basis for further studies of epithelial differentiation during normal and pathologic develo

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01361.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –The effect of treatment with a commercially available 1 % chlorhexidine gel (Hibitane Dental) in custom trays for 14 days (10 min daily) on the sympathetic adrenergic nerves in biopsies from human buccal mucosa was investigated. Chemical assay of endogenous noradrenaline concentration and immunocytochemical demonstration of tyrosine hydroxylase served as markers for structural and functional integrity of the adrenergic nerves. The mean concentration of noradrenaline in chlorhexidine treated tissues did not differ from the mean concentration in control. No morphologic or fluorescence intensity differences of the tyrosine hydroxylase immunoreactivity were found in the chlorhexidine treated tissues when compared to controls. These findings indicate that a relatively intense treatment with a commercially available 1% chlorhexidine gel does not affect the adrenergic innervation of human buccal mucos

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01362.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –The surface ultrastructure of the healthy oral mucosa of humans was studied using SEM as follows: dorsum of the tongue (10 specimens), buccal mucosa (5), floor of the mouth (3), hard palate (5), and gingiva (10). One part of each formalin‐fixed sample was processed routinely using the system of critical point drying for scanning electron microscopy. The other part of the specimen was embedded in paraffin blocks and stained with hematoxylin‐eosin for light microscopy. With SEM at low magnification, the surface structure of the oral mucosa at different areas of the oral cavity was smooth with some desquamating cells. Only the tongue mucosa with its papillae formed a specialized mucosa. The hairs of the filiform papillae were covered by microorganisms, whereas on the oral mucosa there usually was little or no colonization by microorganisms. At high magnification, the surface structure of the superficial epithelial cells was pitted or microplicated. On keratinized epithelium the surface structure was pitted, whereas on non‐keratinized epithelium it was microplicated. On cell boundaries some variation could also be seen; in keratinized epithelium these boundaries were overlapping and in non‐keratinized epithelium they w

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01363.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –Epidemiologic investigations have shown that nicotine could be a factor causing oral melanin pigmentation in man. Nicotine was therefore tested on amphibian skin melanocytes. Cell reactions were registered by spectrophotometry, in vitro light microscopy, photomicrography and light and electron microscopy. It was found that dermal melanocytes in the toad Bufo bufo and the frog Rana arvalis were activated by nicotine and that this activation was not mediated via β‐adrenergic receptors in melanoc

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01364.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –Sixteen edentulous patients were examined before and 2 months after rehabilitation with fixed bridges supported by fixtures in the mandible. Data were collected regarding bite force, chewing efficiency, chewing movements and self‐estimated chewing ability. The results after rehabilitation indicated that patients with an obvious improvement of chewing efficiency showed a greater increase of the mean bite force parameters than patients with unchanged chewing efficiency. However, it was not possible to find any definite pattern of differences between the groups of patients, based on the chewing efficiency index after rehabilitation. Furthermore, after rehabilitation the test group revealed a positive correlation between the duration of the occlusion phase and bite force values, negative correlations between bite force “as when chewing” and closing velocity and between duration of the occlusion phase and opening mandibular velocity and displacement, respe

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01365.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Abstract –The aim of this study was to compare the marginal breakdown as a measure of clinical behavior of fillings made with two conventional amalgam alloys, one of which contained 1% stannous fluoride. Children requiring class II restorations in both mandibular molars received conventional amalgam (New True Dentalloy (R)) in one molar and a fluoride‐containing amalgam (Fluor Alloy (R)) in the other. Impressions of the filled teeth were taken just after polishing and at 1‐yr intervals up to a 2‐yr period. Plastic models were made and evaluated under a stereomicroscope. The results showed that the fluoride‐containing amalgam presented slightly better margins than the conventional amalgam a

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1986.tb01366.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY