摘要:

abstract—The activity of calcium‐stimulated ATPase (E.C. 3.6.1.3) in homogenates of the secretory enamel organ of rat incisors was studied biochemically. ATP hydrolysis was estimated from the amount of inorganic phosphate liberated. An analysis of the total degradation of ATP was initially performed to ensure that the enzyme assays pertained to the original substrate, ATP, and were not influenced by reaction products formed. Standard incubations were run in trismaleate buffer, pH 8.2, with 3 mM ATP, 3 rnM Ca2+and 0.5 mM R8231 at 37°C. The presence of R 8231 was necessary to inhibit nonspecific alkaline phosphatase. The calcium‐stimulated ATPase was completely inhibited when heated at 55–60°C for 5 min. The pH optimum was found to be 8.2. The hydrolysis was substantially dependent on Ca2+and was fastest when the ATP:Ca2+ratio was 1:1. High substrate concentrations inhibited the hydrolysis. The addition of 1 mM Zn2+and Ni2+to the incubation medium markedly inhibited the hydrolysis as did, though less strongly,p‐hydroxymercuribenzoate, oligomycin, EDTA and ruthenium red.l‐Cysteine, mercaptoethanol, iodoacetic acid and sodium azide were without effect. F. was without effect unless added to a final concentration above 15 mM to media where Ca2+had first been allowed to r

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00601.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

abstract—Nitrophenyl phosphate hydrolysis was studied at neutral pH with tissue preparations of the rat secretory and maturation enamel organs and denial pulp. By introduction of inhibitors to nonspecific alkaline phosphatase activity and stimulants to the K+stiinulated and ouabain‐sensitivep‐nitrophenyl phosphatase activity, the latter‐enzyme activity could be demonstrated. This enzyme activity is generally held to be representative of the enzyme sodium‐ and potassium‐stimulated adenosine triphosphatase. The K+‐stimulated activity was magnesium dependent and highly sensitive to fluoride. It was inhibited completely by 3 mM fluoride in the incubation medium and about 1 mM produced hall the maximum inhibition. The K+‐independent enzyme activity was inhibited 50–60% by fluoride in concentrations between 3 and 15 mM. The high fluoride sensitivity of the K+‐stimulated activity may perhaps help to explain the vulnerability of dental

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00602.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

abstract—Following a single application of a chemically polymerized fissure sealant (Concise Enamel Bond System®) 60% of the sealants were present after 2 years of examination. The effectiveness of the treatment was highly significant and the caries reduction, irrespective of the sealant status, was found to be about 5096. The caries reduction was 98% in sealed pairs where the material was fully retain

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00603.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

abstract—Cell‐mediated immunity (CMI) and humoral immunity (HI) against oral bacteria (Streptococcus sanguisstrain 2A, ATCC 10556,Streptococcus sanguisgroup H1,Neisseria subflavaandStaphylococcus) and adult human oral mucosa (AHOM) antigens were investigated in relation to exacerbation of recurrent aphthous stomatitis (RAS). The material consisted of 10 patients with RAS and 10 controls. The CMI and HI were assessed by means of the leukocyte migration test (LMT) and a double‐layer immunofluorescence (IF) technique. Compared with normal controls the leukocyte migration inhibition againstStrep. 2Aand AHOM was significantly increased in patients with exacerbation of RAS and the reactivity seemed to develop in relation to the exacerbation of RAS. The leukocyte migration inhibition was not significantly increased whenStrep. H1, Neisseria subflava, Staphylococcusor fetal human kidney extracts were used as antigens. The IgG antibodies againstStrep. 2AandStrep. H1were significantly raised in sera from patients with exacerbation of RAS. However, the endpoint titers (EPT) did not increase significantly in relation to the exacerbation of RAS. The distribution of EPT againstNeisseria subflava, Staphylococcusand AHOM did not differ significantly when sera from patients with exacerbation of RAS were related to sera from controls. The present investigations support the hypothesis that CMI against streptococcal and AHOM antigens may be involved in the immunopathogenesis o

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00604.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

abstract—The number ofS. mutans(S.m.) in saliva of parents and their children was studied. Thirty‐six children, 4 1/2–5 years old, and their parents participated. A quantitative correlation was found between the number of S.m. in the mothers and their children. The degree of infection also seemed to reflect the caries experience of the child. When adults with different levels of S.m. in saliva contaminated metal spoons with saliva, a correlation between the salivary count of S.m. and the number of microorganisms transferred to the spoon was found. The survival of S.m. on saliva‐contaminated test‐plates was tested. After 7 h the number of viable S.m. had decreased considerably but a few cells could be found after 24–48 h. To study the presence of S.m. on objects in the city often touched by hands, MSB‐containing agar dishes were pressed against several objects such as doorhandles. Only four out of 24 objects were found to be contam

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00605.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

abstract—The rate of migration of leukocytes (OMR) was estimated by counts of sequential mouthrinses in 81 human subjects in order to evaluate its usefulness as a laboratory test of oral inflammation. Periodontitis was taken as a model, and neither patients nor controls had any other oral inflammation. Patients with advanced periodontitis, but with periodontal destruction of about the same level, were divided into two groups, acute phase and chronic phase. The mean number of leukocytes entering the oral cavity in 30 s was estimated in these two groups. In the same patients the difference between the cell count in the first rinse and the OMR level was estimated, to test whether it could be taken to indicate the grade of acuteness of the inflammation (GAI). OMR and GAI were assessed in the patients with periodontitts after the initial treatment and at Follow‐up 1 year after completion of the full treatment, which almost always included surgical procedures. The results indicate that the OMR reflects the existence of oral inflammation and can be used as a laboratory test with the same reservations as white blood cell counts for systemic inflammation. The difference in GAI between patients in the acute and chronic phases of the disease was highly significant, indicating that this test gives information about the severity of the inflammat

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00606.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

abstract—The purpose of this clinical study was to evaluate the effect of a 3‐min daily toothbrushing with 1% chlorhexidine gluconate toothpaste on dental plaque, gingivitis and gingival hyperplasia in 30 mentally retarded epileptic children treated with phenytoin. A double‐blind cross‐over technique was used. Brushing once daily for 2 months with 1% chlorhexidine gluconate toothpaste resulted in significantly lower Pl I and GI values. Gingival hyperplasia was not significantly reduced by the use of the chlorhexidine too

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00607.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY

摘要:

abstract—A clinical, histologic and microradiographic investigation of 62 natal, neonatal and otherwise pre‐erupted teeth from 51 children is presented. Such a comprehensive material has not previously been reviewed. Of the 62 teeth, 59 were mandibular incisors. A hereditary factor could be demonstrated in 12 cases. Fifty‐three teeth showed more or less pronounced enamel hypoplasia. The enamel had a normal prism structure and mineralization with the exception of 22 cases where the prism structure was absent in the cervical part of the enamel. The cervical and apical dentin was atubular and in developing teeth the dentin in these regions changed to an irregularly formed hard tissue of osteodentin character, in which enclosed cells could be observed. Developing teeth often had no cementum, and in those cases where acellular cementum could be observed it was thinner than normal. In four patients atypically located carious lesions occ

ISSN:0909-8836    

DOI:10.1111/j.1600-0722.1978.tb00608.x

出版商:Blackwell Publishing Ltd    

年代:1978

数据来源: WILEY