摘要:

AbstractType VII collagen is a genetically distinct member of the collagen family of proteins. Type VII collagen has been shown to be the major component of anchoring fibrils, attachment complexes which secure the cutaneous basement membrance of the skin to the underlying dermis. Understanding of the structure of type VII collagen has been advanced by recent cloning of the corresponding gene. Chromosomal mapping of the gene to the short arm of chromosome 3 and identification of intragenic polymorphic markers have allowed demonstration of strong genetic linkage between the type VII collagen locus and the dystrophic forms of EB (epidermolysis bullosa). This overview summarizes the progress made in the molecular genetics of type VII collagen.

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00065.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00066.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractInjection of melanoma cells into the UV‐irradiated ear skin of syngeneic mice results in an increased incidence of melanomas compared with that in nonirradiated ear skin. This effect of UV is localized to the site of irradiation and appears to be immunoiogically mediated. In these studies we test the hypothesis that the effect of UV irradiation on melanoma development is related to its ability to alter epidermal Langerhans cells and impair the induction of contact hypersensitivity. A regimen of UV irradiation that altered epidermal immune cells and interfered with the generation of contact hypersensitivity was tested for its ability to increase the incidence of melanoma. Conversely, the ear skin of C3H mice treated with a regimen of UV radiation that enhanced melanoma development was examined for the number of appearance of ATPase+and Thy‐1+dendritic epidermal cells and tested for the ability to initiate a contact hypersensitivity response. No correlation between these effects of UV irradiation could be detected. Furthermore, implantation of melanoma cells into UV‐irradiated ear skin resulted in the generation of systemic immunity against subsequent tumor challenge. Therefore, we conclude that the ability of UV irradiation to modify melanoma development is unrelated to its effects on the afferent arm of the contact hypersensitivity response and that enhanced melanoma development is not due to an impairment in the induction of tumor imm

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00067.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractA soluble form of the usually membrane‐bound adhesion molecule ICAM‐I was detected in supernatants derived from human epidermal keratinocytes. Specifically, supernatants harvested from long‐term cultured normal human keratinocytes, or from the spontaneously immortalized keratinocyte cell line HaCaT, did not contain significant amounts of sICAM‐l, but shedding of sICAM‐1 was found to be markedly induced upon stimulation of keratinocytes with rh IFNγ. In contrast, cells from the two epidermoid carcinoma cell lines. KB and A431, constitutively shed significant amounts of slCAM‐l even without cytokine stimulation, and sICAM‐l contents in supernatants harvested from these cells were further increased upon stimulation of cells with rh IFNγ. These studies indicate, that in addition to peripheral blood mononuclear cells and human melanoma cells, human epidermal keratinocytes constitute an important cellular source of sICAM‐l. By binding to leukocyte LFA‐1 molecules, keratinocyte‐derived sICAM‐l may influence inflammatory responses in the skin. In addition, constitutive shedding of sICAM‐l by transformed human keratinocytes may represent a possible mechanism by which neoplastic keratinocytes

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00068.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractExamination of biopsy samples from 62 patients with – or with suspected – cutaneous T‐cell lymphoma (CTCL) revealed 2 cases in which the neoplastic cells were positive for the T‐cell receptor (TCR) γδ complex. One patient had mycosis fungoides and 1 patient had a pleomor‐phic lymphoma of medium and large‐cell type. Both cases showed aggressive courses with dissemination to internal organs and short survival times. The phenotypic examination showed that the neoplastic cells were positive with TCRδ1, CD3, CD25, CD29, CD45RO and CD54. No staining was seen with antibodies against framework determinants or variable regions on the TCR αβ heterodimer. Negative reactions were also seen with CD4, CD8, CD5, CD7, CD16, CD30 and CD57. It is concluded that rare CTCL express TCR γδ chains. These malignancies may originate from the TCR γδ‐positive T cells seen in normal skin, and it is possible that their recognition may be importan

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00069.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractRecent investigations indicate that malignant melanoma cells can produce distinct cytokines. While differences in the production of single cytokines have been observed among different melanoma cell lines, the extent of variability in the production of single and multiple cytokines between individual melanoma cell lines has not been as thoroughly investigated. A heterogeneity in melanoma cell cytokine production could have important implications for the biology of this aggressive neoplasm since certain cytokines may act as autocrine growth factors or be potent modulators of host immune response to the developing tumor. The purpose of this study is to assess the cytokine production profile of two widely available human melanoma cell lines, A375 and G361. The A375 cell line constirutively expressed the mRNA for IL‐1α, IL‐1β and PDGF‐A, with increased expression of these cytokines after induction with PMA. GM‐CSF mRNA was expressed by the A375 melanoma line only after induction with PMA. No IL‐6 mRNA was detected in the A375 melanoma cell line. The cell culture supernatants from the A375 cells likewise contained a parallel increase in IL‐1 activity as determined in the D10 bioassay and secreted GM‐CSF and PDGF‐AA as measured by ELISA. In contrast, the G361 cell line did not express IL‐1, GM‐CSF or PDGF‐A mRNA (constitutively or after PMA induction) but expressed only IL‐6 mRNA and secreted IL‐6 activity after PMA induction. These results demonstrate a significant heterogeneity in the production of IL‐1α, IL‐1β, IL‐6, GM‐CSF, and PDGF in two distinct melanoma cell lines. This study demonstrates that individual melanoma cell lines express and secrete multiple cytokines both constitutively and after stimulation with PMA. The immunomodulating and mitogenic properties of these melanoma‐derived cytokines may have implications in determining the biologic be

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00070.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract5‐methoxypsoralen (5‐MOP) is considered an alternative to 8‐methoxypsoralen (8‐MOP) for photochemotherapy of psoriasis. We have compared the clinical efficacy and tolerability of 5‐MOP (1.2 mg/kg)‐UVA versus 8‐MOP (0.6 mg/kg)‐UVA therapy in 25 patients of skin type III and IV, affected by relapsing plaque‐type psoriasis of similar body involvement; indeed, the same patients were given 8‐MOP during 1 year and 5‐MOP during the subsequent year after relapsing. Both treatments cleared psoriatic lesions with a comparable number of exposures, but 5‐MOP required significantly higher cumulative UVA doses. The difference was due to the lower phototoxicity of 5‐MOP, as assessed by the determination of the minimal phototoxic dose, and to its higher tanning activity, as assessed by the weekly grading of pigmentation. Nevertheless, therapy by 5‐MOP‐UVA seemed particularly interesting in that it showed a higher tolerability since only 1 patient experienced nausea, whereas during therapy with 8‐MOP‐UVA nausea and/or vomiting occurred in 7 patients, sunburn in 6 and itching in 3. Since we have treated the same patients with the two drugs, our results were not influenced by interindividual variations of phototoxic responses, tanning ability and susceptibility to develop psoraleninduced short‐term side‐effects. It was concluded that, although long‐term side‐effects of the 5‐MOP‐UVA treatment have still to be determined, such treatment of psoriasis should be reappraised due to its higher tole

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00071.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

AbstractAn unexpected finding concerning our previously reported polyclonal antibody raised against an extract from human amnion (pAb 6/2, also termed AA3), and which recognizes an epidermal keratinocyte protein, is presented in this study. Using the immunoblot technique, pAb 6/2 binds to a 37 kD intracellular protein antigen. We have subsequently found that, by radioimmunoprecipitation performed after metabolic labelling with35S‐methionine of cultured keratinocyles, pAb 6/2 recognizes the 600 kD epidermal basement membrane component (termed BM‐600/nicein) which was reported to be bound by the monoclonal antibody mAb GB3. Specifically, pAb 6/2 reacts with immunoaffinity chromatography‐isolated BM‐600/nicein blotted onto nitrocellulose. The data suggest the existence of two immunological reactivities borne by pAb 6/2, each of them being directed against, respectively, the 37 kD (seen in immunoblots) and the 600 kD protein (seen in immunoprecipitations). The data further suggest possible independent expression of these two proteins in cell culture. In comparison with the staining pattern of normal skin, immunofluorescence was previously noted to be impaired (pAb 6/2) or absent (mAb GB3) in lethal junctional epidermolysis bullosa. Thus, we conclude that mAb GB3, rather than pAb 6/2, is a more appropriate probe for the comprehensive biochemical study of this genoder

ISSN:0906-6705    

DOI:10.1111/j.1600-0625.1992.tb00072.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY