摘要:

AbstractWe have analysed TFP3 transposable elements from five independently isolated FP mutants of theAutographa californicanuclear polyhedrosis virus (AcMNPV). We also analysed genomic copies of TFP3 elements amplified from the DNAs of theTrichoplusia nicell line (TN‐368) andT. nilarvae using the polymerase chain reaction (PCR). The sequences of all the newly isolated TFP3 elements closely resemble the previously described TFP3/1 element. Each of the transposons isolated from the virus mutants duplicated a TTAA tetranucleotide target site upon insertion into the viral genome. Four of these TFP3 elements transposed into three different ‘TTAA’ target sites within the 25 K gene (FP locus, map units 36–37 of AcMNPV). The fifth TFP3 element inserted at a ’TTAA’ site within the AcMNPVHindlll‐E fragment. One genomic TFP3 element, amplified from the TN‐368 cell line DNA by an inverse PCR method, duplicated a ‘TTAA’ tetranucleotide target site that is present only once in the homologous larval DNA sequence. These data suggest that mobilization of TFP3 into both viral and cellular sites is identical in specif

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1993.tb00111.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractAnalysis of restriction fragment length polymorphism (RFLP) in mitochondrial DNA (mtDNA) in a population ofLeptothorax acervorumdemonstrates substantial population substructuring. Digestion with four restriction endonucleases, Haelll, Mbol, Mspl and Rsal, gave six, four, three and two different patterns, respectively. Seven composite haplotypes were obtained from the observed cleavage patterns. Uniform aggregations of nests from the same maternal lineage (i. e. with the same haplotype) were found which suggests that nestfounding by a process of budding‐off is common. Groups of nests seem to actively exclude other haplotypes from establishing nests within their territory. Fstatistics suggests that individuals mate at random within the populatio

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1993.tb00112.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractTwo isolates of bacterial endosymbionts, GP01 and GM02, were established in cell free medium from haemolymph of the tsetse,Glossina pallidipesandG. morsitans.These microorganisms appear similar to rickettsia‐like organisms reported previously from various tsetse species. The 16s rRNA sequence analysis, however, placed them within the gamma subdivision of the Proteobacteria, phylogenetically distinct from most members of the Rickettsiaceae which align with the alpha subdivision. Distinct multiple endogenous plasmids are harboured by GP01 and GM02, suggesting that the two isolates are different. Restriction mapping analysis showed that one of the conserved plasmids is present in high copy number and is at least 80 kb in size. A heterologous plasmid pSUP204, which contains the broad host rangeoriVreplication origin, was used to transfect bacterial cultures. The symbiont GM02 was transformed, and it expressed plasmid encoded resistance to the antibiotics ampicillin, tetracycline and chloramphenicol. Transformation of these symbionts may provide a novel means for expressing anti‐parasitic genes within tsetse populati

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1993.tb00113.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractSelective enrichment has been used in a number of instances for the isolation of species‐specific sequences in prokaryotes. This paper reports the successful application of the technique to insects. Genomic probes were derived to the target speciesD. funebrisandD. simulans. The method involves the biotinylation of non‐target ‘driver’ DNA prepared from the closely related speciesD. melanogasterand its hybridization to homologous sequences in the target DNA. Hybrid molecules were removed from the reaction by incubation with streptavidin followed by phenol extraction, leaving a preparation enriched for target fragments. All DNA fragments isolated in theD. funebrisexperiments proved to be specific to that species. Five out of twenty‐four fragments screened in theD. simulansexperiments were specific when screened with homologous DNA and genomic DNA from its sibling species,D. mel

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1993.tb00114.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractA glycoprotein (BM86) from the gut cells of the cattle tickBoophilus microplus, when used to vaccinate cattle, has been shown to protect cattle from tick infestation. A recombinant BM86 protein is the principal component of a novel tick vaccine currently under development. The nature of the anchorage of BM86 to tick gut epithelial cells has been investigated using BM86 fromB. microplusand recombinant BM86 proteins expressed in insect cells using the baculovirus expression system. BM86 fromB. microplusand a full length recombinant BM86 are shown to be anchored to the extracellular surface of tick gut epithelial cells and baculovirus‐infected insect cells, respectively by a glycosyl‐phosphatidyl inositol membrane anchor. A recombinant BM86 truncated by the removal of a hydrophobic region coding for thirty amino acids at the carboxy‐terminal end was secreted from baculovirus‐infected Sf9 cells. This secreted form of recombinant BM86 showed strong protective activity against ticks in cattle vaccinated with this

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1993.tb00115.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractIn haematophagous insects digestion of the blood meal provides nutrients for survival and essential components for egg production. We have isolated and partially characterized two gut‐specific genes from the black flySimulium vittatum. Sequence analysis revealed that both are highly similar to digestive proteases, one to trypsins and the other to carboxypeptidases. RNA blot analysis indicates that the expression of these two genes is regulated in a sexspecific manner; when fed the same sucrose‐based diet, expression in males is substantially lower than in females. In females, expression of both genes is strongly induced by a blood meal. At 6 h after the blood meal the trypsin‐like gene product was immunolocalized to the midgut epithelium and to the outer layers of the peritrophic m

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1993.tb00116.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

AbstractThe polymerase chain reaction was used to amplify six mitochondrial t‐RNAs for Ala, Arg, Asn, Ser, Glu and Phe between genes for mitochondrial NADH dehydrogenases 3 and 5. With respect toDrosophila yakubathe gene order and direction of transcription is completely conserved. Analysis of secondary structure shows complete conservation of the anticodon loops but a number of differences in the dihydrouridine and TψC loops with respect toDrosophila. However, differences are such that tertiary interactions that stabilize stacking are preserved. The use of the reported sequence in combination with PCR to explore population variability is discuss

ISSN:0962-1075    

DOI:10.1111/j.1365-2583.1993.tb00117.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY