ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01098.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTLymphocyte transformation by varicella‐zoster virus antigen was investigated in 43 blood samples from herpes zoster patients without serious underlying diseases. Lymphocyte responses were low in the early days of the illness and increased in the convalescent stage with wide dispersion. Although the intensity of the skin test and complement fixing antibody became mostly positive on coincident convalescent days, no distinct correlation was observed in the values of these three immunological indexe

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01099.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe purpose of the present study was to examine the phenotype of cutaneous immunocompetent cells and to quantify Langerhans cells in Lichen planus by the use of monoclonal antibodies directed against T‐cell populations.Helper cells (OKT4+) and Suppressor/cytotoxic cells (OKT8+) were observed in all cutaneous infiltrates, and numerous Langerhans cells were identified by OKT6, BL6, and BL2 (HLA‐DR) in the epidermis and dermis.The quantification of Langerhans cells demonstrated that the number of dendritic cells in epidermis is greater in involved skin than in non‐involved skin. In recent lesions, Langerhans cells are more abundant than in older lesions.The results in peripheral blood indicated a T Helper/Suppressor imbalance with a decreased T‐Suppressor/cytotoxic subpopulation in patients with lichen planus diseases.In lichen planus, our results suggest an immunological reaction involving all the immunocompetent cell subpopulations with a first stage of information by Langerhans cells (OKT6+, BL6+, BL2+ (HLA‐DR)) and Helper cells (OKT4+), and second stage mediated by Suppressor/cytotoxic cell

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01100.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTPulse‐chase labeling experiments with3H‐histidine and3H‐arginine were performed in hairless mice to investigate the origin of free amino acids in the stratum corneum. Time‐course labeling of epidermal proteins was examined by sodium dodecyl sulfate‐polyacryl amide gel electrophoresis (SDS‐PAGE). The radioactivity was also measured in the following three epidermal fractions; 0.1 N HClO4soluble‐ethanol soluble fraction (Fr. I), 0.1 N HClO4soluble‐ethanol insoluble fraction (Fr. II, histidine‐rich protein fraction) and 0.1 N HClO4insoluble‐8M urea soluble fraction (Fr. III).Labeled amino acids were rapidly incorporated into Fr. III, especially into a particular protein which showed little mobility on SDS‐PAGE. As the radioactivity of this protein decreased, an increase in radioactivity was observed in a 32,000 MW histidine‐rich protein, the only major protein in Fr. II. The radioactivity of 32,000 MW protein then fell, accompanied by an increase in radioactivity of Fr. I. The major radioactive substances in Fr. I were identified as3H‐histidine and3H‐urocanic acid after3H‐histidine injection and as3H‐arginine after3H‐arginine injection. The amino acid composition of the histidine‐rich protein was very similar to the composition of free amino acids of the stratum corneum, in which amino acid metabolites were considered as their precursor amino acids.These results suggested that the free amino acids of the stratum corneum might be a final product of the de

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01101.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTIncreased adenosine deaminase activity was observed in peripheral lymphocytes from a patient with adult T cell leukemia. Gel filtration pattern on a Sephadex G‐150 revealed that the enzyme from normal lymphocytes existed in two forms with different molecular weights (large and small), whereas the enzyme from the patient existed solely as the small molecular weight form. Comparison of this small form adenosine deaminase from the patient with that from normal controls revealed that both enzymes were quite similar in relative substrate specificity, Km values for adenosine, pH optima, heat stability pattern, and sensitivity to inhibition by coformycin, a tight binding inhibitor of adenosine deaminase. Increased adenosine deaminase activity and the lack of the large form of the enzyme might be significant features of adult T cell leukemia lymphocyte

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01102.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTThe dorsal resting hair of C3H mice was shaved, activating the hair into the anagen stage. Hair regrowth in mice of different ages (10–20 mice in each group), whose hair cycles were in the telogen stage, were compared after shaving. In both groups of mice, new hair growth was not uniform, but irregular on the shaved areas. Delay in hair regrowth increased as the mice became older (20<66<188<312 day old mice). Furthermore, eighteen 59 day‐old mice were used for biochemical analysis. Nine days after shaving, 30–50% of the shaved skin area was covered with new hair. After sacrifice, the dorsal skins were separated into hair re‐growing, non‐growing, and non‐shaved control areas. Several enzyme activities in the extracts from the above‐named areas were compared in terms of “per g of wet weight tissue”, “per mg DNA” and “per mg of soluble protein”. The activities of ornithine decarboxylase (ODC), transglutaminase (TGase) and alkaline phosphatase (Al‐P) were remarkably high in the extract from the hair re‐growing area as compared with that from the non‐growing area. Comparison of the activities of the follicle‐rich fractions from the hair re‐growing area to those of the non‐shaved area showed that ODC, TGa

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01103.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTForty‐six patients with various skin diseases applied 0.064% betamethasone dipropionate (BD) ointment and cream simply for 3 to 6 weeks, and serum cortisol, blood sugar, and peripherocirculatory eosinophyll counts were measured.Serum cortisol was almost unaffected by the application of BD ointment or cream at the rate of 10 g a day. After using between 10 and 20 g a day a reducing trend in cortisol levels was observed with BD ointment, but not with BD cream. When the application was increased to 20 g a day or more, a marked reduction was noted. This reduction was temporary and the serum cortisol returned to the normal level in no more than one week after the treatment. Peripherocirculatory eosinophyle counts decreased with application of even less than 10 g per day, and then markedly decreased further as the dose increased. Blood sugar was not increased by BD ointment or cream.Topical corticosteroid treatment is generally used by outpatients who usually receive less than 10 g of medication per day. Thus, it was presumed that, for outpatients, simple application of BD ointment or cream should not cause any systemic effects, and that, even if there were effects, they would only be slight, as far as suppression of adrenocortical functions was concerne

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01104.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTHyaluronic acid synthetase (HAS) activity of lyophilized skin from a 49‐year‐old Japanese man with lichen myxedematosus was assayed. The HAS activity was very high in both diseased and normal appearing s

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01105.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTA case of malignant fibrous histiocytoma is reported. Due to the histological features of cellular pleomorphism and myxoid degeneration seen also in other soft tissue sarcomas (rhabdomyosarcoma, liposarcoma), differential diagnosis was difficult. However, electron microscopic studies led to a distinct diagnosis of malignant fibrous histiocytoma.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01106.x

年代:1983

数据来源: WILEY

摘要:

ABSTRACTA case of hypereosinophilic syndrome complicated by malignant lymphoma is reported, including autopsy results. The malignant lymphoma was pleomorphic and characterized clinically and histologically by its unusual localization in the brain. The disturbed immune system of the patient, who had been immunologically compromised by immunosuppression therapy consisting of predonisolone and azathioprine, seemed relevant to the pathogenesis of this malignant lymphoma.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1983.tb01107.x

年代:1983

数据来源: WILEY