ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01532.x

年代:1985

数据来源: WILEY

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01533.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTAntigen‐primed T lymphocytes require accessory cells to initiate a proliferative response. These cells have been designated antigen presenting cells. In the present investigation, we found that immune associated antigen (Ia) bearing, mouse epidermal cells possess antigen presenting activity. These epidermal cells also display substantial levels of mixed lymphocyte reaction (MLR) activity. However, this MLR of itself did not suppress nor enhance antigen presenting activity. Genetic mapping studies demonstrated that compatibility at theI‐AandI‐Esubregions of the MHC is important for effective presentation of either lysozyme or ovalbumin.In vitroinhibition and blocking studies using alloantisera confirmed thatI‐AandI‐Egene products of these epidermal cells may play an important role in the interaction of primed T lymphocytes and antigen‐pulsed epidermal cells. In addition, epidermal cells bearing Fc and C3 receptors on the cell surfaces were more efficient at inducing the lymphocyte proliferation than were epidermal cells depleted of these cells. These results suggest that Ia positive epidermal cells may be involved in the initiation of delayed type hypersensitivity reactions through antigen recognition followed by antigen presentation and T lymphocyte pr

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01534.x

年代:1985

数据来源: WILEY

摘要:

ABSTRUCTPassive transfer of experimentally induced drug‐rash (GR) was studied in JY‐1 strain guinea pigs. Sulbenicillin (SBPC)‐induced GR was transferred into four of ten recipients by SBPC immunized cells. CET‐induced GR was transferred into twelve of seventeen recipients by CET‐immunized cells and accidentally transferred into two of fifteen recipients by CET‐immunized serum. However, CET‐induced GR was not successfully transferred by SBPC‐immunized cells, complete Freund's adjuvant (CFA)‐immunized cells, frozen and thawed materials, or sonicated cells of CET‐immunized cells. Anti‐CET antibody of CET‐immune serum was not detected by passive hemagglutination assay.CET‐immunized cells were separated into adherent and nonadherent fractions (Ad and Non‐Ad) in laboratory dishes coated with anti‐guinea pig γ‐globulin. CET‐induced GR was not transferred by Ad, but was transferred into eight of twenty‐two recipients by Non‐Ad. Only 4.6% of Non‐Ad cells were immunoglobulin bearing. Transferred GR appeared about 19–24 hr after GR‐elicitation. Erythema was paler than that of GR in actively sensitized guinea pigs.Microscopic examination of the skin taken about 24 hr after the cell transfer with systemic challenge revealed edema, dilation and compaction of capillaries, and diffuse lymphoid cell infiltration with a few eosinophils and basophils in the upper dermis. After about 48–72 hr, basophils were not confirmed as in actively sensitized animals. The count of peripheral leukocytes increased until 24 hr after GR elicitation in both recipients of immunized cells and immunized serum regardless of the severity of the GR reaction. It is likely that, in the transfer of drug‐induced GR, immune cells (probably primed T cells) which do not bear immunoglobulin have a significant role in GR, but serum factors a

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01535.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTIn mammals, melanin formation occurs only in melanosomes within the melanocyte, where tyrosinases are most highly accumulated; however tyrosinases exist as precursors of melanosomal enzymes in microsomes, where the enzymes are inactivated by unknown mechanism(s). The Km value of melanosomal tyrosinase for tyrosine was 0.6 mM, but that of the microsomal enzyme was 1.3 mM. The Km for tyrosine of microsomal tyrosinase was lowered to that of melanosomal tyrosinase after incubation of the microsomal enzymes with a Golgi fraction isolated from mouse melanoma. Further, the molecular weight of microsomal tyrosinase appeared to increase after incubation with the Golgi fraction; microsomal tyrosinase showed a slower mobility in SDS Polyacrylamide gel electrophoresis after incubation with the Golgi fraction than before incubation. These data suggest the possibility that microsomal tyrosinase is processed in the Golgi area during transfer to the melanosome in the melanocyte.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01536.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTSusceptibilities ofStaphylococcus aureusstrains derived from furuncle and bullous impetigo to Ampicillin, Cephalexin, Erythromycin, Tetracycline and Chloramphenicol were studied by examining their minimum inhibitory concentrations. Susceptibilities to every antibiotic studied were lower among the furuncle group than bullous impetigo group. This result is considered to be due to differences in the types of strains causing the two pyodermas. It was also indicated in this study that the susceptibilities to the antibiotics (Ampicillin, Cephalexin, Erythromycin and Tetracycline) which have been used in greater amounts were higher than that to the antibiotic (Chloramphenicol) which has been used in lesser ones.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01537.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTA method for measuring skin elasticityin vivobased on repeated applications of suction to a circular skin area with a diameter of 10 mm and measurement of tensile distensibility (TD), hysteresis (H), and resilient distension (RD) by the principle of electrical capacitance calibrated to elevation of the skin surface in mm is introduced. Measurements in 14 patients of 51 lesions of localized scleroderma (morphoea) and regional controls revealed a characteristic rheological pattern in the sclerotic state representing structural alterations with reduced TD and H; H became temporarily increased during remission. Measurements in control regions showed significant correlations between TD and H (r=0.382), and between H and RD (r=0.420). Regional differences between extremities, abdomen, and back were significant and “inversely” correlated to skin thickness as measured by ultrasound (r=0.451). The method was highly reproducible according to studies on silicone rubber membrane (SD of TD different sites, 0.023 mm; SD of H, 0.010 mm; SD of TD same sites repeated, 0.010 mm). The biological, day‐to‐day variation in different regions of a healthy individual was considerably higher (SD of TD, 0.048–0.087 mm), in particular with respect to H (SD, 0.122–0.277 mm). TD was very variable. Experiments on abdominal skin showed that previous mechanical stress resulted in a characteristic pattern with an increase in TD and a decrease in H. Acute edema (histamine weal) resulted in a characteristic pattern with increased TD and H. Both experiments illustrated functional aspects of skin rheology overlying the structure and explaining part of the biologica

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01538.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTGranuloma annulare (GA) and sarcoidosis are two diseases of unknown causes with epithelioid cell granuloma formation. In this paper, we discuss a patient with manifestations of GA and both systemic and cutaneous sarcoidosis. We examined serum MIF and serum MAF to study the relationship between GA and sarcoidosis. Only the serum from a patient in the active stage of sarcoidosis had significant MIF activity. Additional significant MAF activity was found in the active stages of sarcoidosis and GA, especially the stage of developing GA. It may be characteristic that GA has a high potential MAF activity.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01539.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTIn urine samples of patients with Fabry's disease (angiokeratoma corporis diffusum, ACD) mulberry cells appear, exhibiting the ‘Maltese cross’ with polarized light; they are said to be characteristic of this disease. However, their diagnostic significance has never been clearly defined. It is also well known that patients with this disease have electron‐dense periodical lamellar bodies in the cells of various organs. Several electron microscopic investigations have demonstrated such lamellar bodies in urine samples from hemizygous male patients, but it remains unclarified whether heterozygous female carriers excrete similar bodies in urine. We examined urine samples from a female carrier of ACD and two hemizygotes with the disease, as well as from three patients with nephrotic syndrome. With the light microscope, mulberry cells with ‘Maltese cross’ were detected in all six patients, suggesting that these cells are not diagnostic for ACD. With the electron microscope, concentric lamellar spheres were found in the heterozygous female carrier and two hemizygous males, but not in patients with nephrotic syndrome. This is the first report describing urinary laminated bodies in a female carrier of ACD. It is concluded that the demonstration of mulberry cells with ‘Maltese cross’ as well as concentric lamellar bodies in urine is helpful in detecting female carriers of ACD and hemizygotes as young as 7 years of age, before they exhibit definite clinical m

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01540.x

年代:1985

数据来源: WILEY

摘要:

ABSTRACTA 45‐year‐old female patient developed 10 verrucous lesions on the face, neck, back and forearms. Diplopia and occasional headaches occurred at the same time. Examination of the skin lesions by histopathology, direct immunofluorescent study and tissue culture showed cutaneous Cryptococcosis. Meningeal cryptococcosis was also found. Complete cure was effected by a two‐month‐course of Amphotericin B and 5‐fluorocytosine as combine

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1985.tb01541.x

年代:1985

数据来源: WILEY