ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02004.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTAnin vitromethod for assessing the activity of epidermolytic toxin (ET) was developed in this study. It was almost equal in sensitivity to thein vivoassay system in which ET was injected subcutaneously into new‐born mice. By using thein vitromethod it was found that ET could induce epidermal splitting after incubation with the skin only for 5 minutes. No epidermolytic activity remained in the reaction mixture. The activity of ET was not abolished by proteinase inhibitors such as Trasylol,ε‐aminocaproic acid or soy bean trypsin inhibitor. It was abolished by an extract from the epidermis of new‐born mice. The inhibitory activity of the extract was preserved by heating at 56°C for 30 minutes. It was destroyed by heating at 80°C for 30 minutes. The activity was found in a subcellular fraction which should contain mitochondria and lysosomes from the epidermal cells. A reduction in the amount of ET was observed in acrylamide gel electrophoresis after incubation of ET with the subcellular fraction. The possible mode of action of ET was d

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02005.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTTrypsin separation of the epidermis from the dermis was applied to 14 benign, premalignant, and malignant epithelial skin tumors. The three‐dimensional architecture of the undersurfaces of these tumors was clearly observed in all cases.Seborrheic keratosis showed network‐like structures in young lesions and a reticulated type of lesion, but showed sharply demarcated mass‐like structures in well‐developed lesions and an acanthotic type of lesion.Bowen's disease, Paget's disease, and senile keratosis exhibited coarse network‐like structures similar to each other accompanied by a general decrease in the amount of melanin pigment. Boundaries between these tumors and the surrounding epidermis were more or less irregular and gradual.In superficial basal cell epithelioma, interconnections between tumor‐cell nests were likely to exist.It is suggested that this method may fill a gap between macroscopic and microscopic findings, and that it is of considerable use for detecting invasive changes, for determining interrelationships between tumors and skin appendages, and for deciding ranges of surgical

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02006.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTCirculating immune complexes were detected by the polyethylene glycol precipitation method in the sera of patients with antibodies to ribonucleoprotein (RNP). Levels of immune complexes were significantly higher than in normal control sera and correlated well with disease activity as defined by clinical manifestations, decreased levels of complement and increased fluorescent anti‐nuclear antibody titers.Analysis of IgG‐aggregates in the sera of patients revealed the following characteristics: molecular weight of about 900,000 daltons, sensitivity to RNase, resistance to DNase, no Clq‐binding activity and no RF act

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02007.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTThe antibody titer toPityrosporum orbiculareof 40 patients with pityriasis versicolor was measured using both the sensitized hemagglutinin method and the immunofluorescence antibody technique. Among the patients there was no significant correlation between the antibody titer and the lesional duration, distribution, color, or the presence or absence of the itching. When compared with the antibody titer of 31 healthy volunteers, no significant differences between the groups were found. In 18 out of the 40 patients, biopsy specimens were prepared for light microscopy and direct immunofluorescence microscopy. Acanthosis was observed in 13 specimens but no deposits of immunoglobulin or complement were noted.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02008.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTYoung adult mice were inoculated intraperitoneally with 30LD50ofHerpes simplexvirus type 1 (Miyama strain). They died within 14 days after the inoculation. Mice were equally protected by intraperitoneal injection of L cell interferon (L‐IF) or mouse brain IF (MB‐IF) given 1 day before, simultaneously and 1 day after the viral inoculation. Mice were also protected when 100μg of polyinosinic acid‐polycytidylic acid (Poly I:C) was given intraperitoneally 1 day before the viral inoculation or on the same day, but not if given on the following day. When adenine arabinoside (ara‐A) was administered every day from the 1st to the 4th day after the viral inoculation, the protective effect also occurred. However, lipopolysaccharide (LPS), amphotericin B methyl ester (AME) and gamma globulin (GG) were ineffective in reducing mortality. The protective effect could be also demonstrated by combining poly I:C and L‐IF at doses which were individually ineffective. On the other hand, no synergism between L‐IF and ara‐

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02009.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTThe serum levels of the four immunoglobulins, IgG, IgA, IgM and IgE, in Guatemalan onchocerciasis patients were determined quantitatively using a laser immunoassay system or radioimmunosorbent test.The IgG and IgE levels in Guatemalan onchocerciasis patients positive forOnchocerca volvulus microfilariaewere significantly higher than the levels in Guatemalan controls negative formicrofilariaeand in healthy Japanese controls. But, no differences in the levels of IgA and IgM were obtained between the Guatemalan onchocerciasis patient group and the two control groups.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02010.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTThirty‐eight donors were selected from 312 volunteers to be as nearly compatible as possible with the patient's HLA‐A and ‐B antigens.In addition to HLA‐A and ‐B, MLC test was performed between the patient and all but one of the 38 donors (Donor No. 35), and all 38 homografts were applied to the patient at once.The mean survival time of HLA‐A and ‐B‐identical grafts was better than two haplotype‐mismatched grafts. There were no clear differences between 0 and 1, or 1 and 2 haplotype‐mismatched grafts.No definite differences in the survival number at 21 days was observed between HLA‐A and ‐B‐identical grafts and 1 haplotype‐mismatched grafts. However, a significant difference was detected between (0+1) mismatched grafts and 2 mismatched grafts.We were not able to detect any effects of ABO blood antige

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02011.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTA case of a 77‐year‐old male with Sézary's syndrome (SS) is reported, including ultrastructural and immunohistochemical characterization of the cellular infiltrates.Atypical lymphocytes in the skin were evaluated at stages of both clinical exacerbation and improvement. During both stages, skin specimens revealed essentially similar findings. They were diffusely infiltrated by atypical lymphocytes with a characteristic nuclear convolution. The infiltrating cells exhibited T‐cell markers which cross‐reacted with the T‐cells of the lymph nodes. Atypical convoluted cells in the peripheral blood were PAS positive and peroxidase negative. The morphological and functional characteristics of the cellular infiltrates in SS and its clinical independence from mycosis fungoides were

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02012.x

年代:1981

数据来源: WILEY

摘要:

ABSTRACTA 41‐year‐old Japanese man with a 11‐year history of Hailey‐Hailey's disease on his groin developed generalized herpes simplex on his groin, throat, lips and extremities which, to our knowledge, has not been previously described.Histological examinations using light and electron microscopes showed herpes virus infection of the skin. Virus culture and serological methods revealed this case to be a primary infection of type 1 herpes simple

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1981.tb02013.x

年代:1981

数据来源: WILEY