ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01401.x

年代:1994

数据来源: WILEY

摘要:

AbstractThe effects of PSK on tumorigenesis in mouse skin were investigated either when mouse skins were initiated by benzo(a)pyrene and promoted by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (Group I, two‐stage carcinogenesis) or when both initiated and promoted by benzo(a)pyrene (Group II, complete carcinogenesis). Twelve mice in each group were fed chow with or without 0.4% PSK. This concentration of PSK was determined by calculation to give mice enough PSK to exert antitumorigenic activity without cytotoxicity. By the end of the experimental periods (26 weeks), two carcinoma‐burdened mice in Group I without PSK were dead, but no carcinomas at all were identified in the mice fed with PSK, although considerable numbers of papillomas developed in both groups. In Group II, carcinomas started to evolve at the 15th week of the experiment regardless of PSK feeding. The number of carcinomas observed in the mice fed with PSK in Group II was statistically significantly lower than that in the mice fed without PSK. Histologically, mild inflammatory infiltrations were seen around the papillomas, and moderate to dense infiltrations, mainly composed of neutrophils, T lymphocytes, and macrophages, were observed in squamous cell carcinomas. There were apparently no significant differences in the number of the infiltrating cells around carcinomas in PSK (+) and PSK (−) groups in both early and fully developed lesions. However, considerable numbers of cells infiltrating into the nests were observed in the early lesions of elicited carcinomas in the mice fed with PSK, while such cells were rarely seen in carcinoma nests in the group without PSK at that stage. The multi‐stage carcinogenesis regimen which evokes both benign and malignant epidermal tumors in mouse skin should provide insights into the function of the specific infiltration of immuno‐competent cells and should also be a valid system for the quantitative and qualitative analysis of the anti‐tumor effects of immunopot

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01402.x

年代:1994

数据来源: WILEY

摘要:

AbstractWe have applied DNA flow cytometric analysis to paraffin‐embedded tissue sections of primary malignant melanomas. Conventionally, flow cytometric analysis of paraffin‐embedded tissue sections has been done by the method of Hedley et al. We added ultrasound treatment to the method of Hedley et al. and a lower value of coefficient of variation was shown. Furthermore, a new technique, fluorescencein situhybridization with a chromosome‐specific repetitive DNA probe, was used for the analysis of chromosomal numerical aberrations in the same paraffin‐embedded tissue sections. The DNA flow cytometric analysis showed that in 8 cases six primary malignant melanomas were of the aneuploid pattern and two cases of lentigo maligna (melamonain situ) were of the diploid pattern. By fluorescencein situhybridization, the two cases with the diploid pattern had spots/nucleus of 1.28 and 1.12, and those with the aneuploid pattern had spots/nucleus from 2.01 to 2.27. Only one nodular melanoma in an aneuploid case showed spots/nucleus of 1.71. These data indicate that fluorescencein situhybridization with chromosome‐specific repetitive DNA probes can serve as a cytogenetic tool for the analysis of interphase nuclei of solid human tumors and may be useful for the study of tumor cell hete

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01403.x

年代:1994

数据来源: WILEY

摘要:

AbstractWe performed an immunohistochemical study of BCA‐225, which is a glycoprotein secreted by the T47D breast carcinoma cell line and recognized by monoclonal antibody BRST‐1 (clone name: CU‐18), in normal skin and various skin cancers. In normal skin, BCA‐225 was positive only in the secretory portion of both eccrine and apocrine glands and in mature cells of the sebaceous gland. We observed 10 cases of squamous cell carcinoma of the skin, 10 cases of basal cell carcinoma without sebaceous differentiation, 3 cases of basal cell carcinoma with sebaceous differentiation, 6 cases of malignant trichilemmoma, 8 cases of eccrine porocarcinoma, 3 cases of ductal carcinoma, 1 case of malignant clear cell hidradenoma, 1 case of apocrine adenocarcinoma, 6 cases of extra‐ocular sebaceous carcinoma, 5 cases of extramammary Paget's disease with underlying adenocarcinoma, and 11 cases of extramammary Paget's disease without underlying adenocarcinoma. Most of the cases of sweat gland carcinoma, basal cell carcinoma with sebaceous differentiation, sebaceous carcinoma, and extramammary Paget's disease were positive for BCA‐225, while none of the cases of squamous cell carcinoma, basal cell carcinoma without sebaceous differentiation, or malignant trichilemoma were positive. Based on these findings, we believe that BCA‐225 is useful in distinguishing tumors with sweat gland and sebaceous differentiation and extramammary Paget's disease from tumors without such di

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01404.x

年代:1994

数据来源: WILEY

摘要:

AbstractSkin biopsies of graft‐versus‐host reaction (GVHR)‐type drug eruptions in the acute phase were compared immunohistochemically with those in the chronic phase and also with non‐GVHR type drug eruptions in the acute phase. Predominance of CD8+T cells in the epidermal infiltrates, reduction in the number of epidermal OKT6+dendritic cells (Langerhans cells), and increased expression of HLA‐DR and ICAM‐1 on keratinocytes were observed in the acute phase of GVHR‐type, but not in either the chronic phase of GVHR‐type or the acute non‐GVHR type. These findings were similar to those of previous reports on skin lesions of acute GVH disease (GVHD) seen after bone marrow transplantation.Therefore, immunohistochemistry is not useful for differential diagnosis between acute GVHR‐type drug eruptions and acute cutaneous GVHD. These findings also indicate that similar immunomechanisms may be involved in the pathogenesis of both GVHR‐type drug eruptio

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01405.x

年代:1994

数据来源: WILEY

摘要:

AbstractAn 11‐year‐old boy presented with inflamed tinea pedis followed by a generalizedid typeskin eruption, with disappearance of the rash after treatment of the mycotic foot lesions. The pathogenesis of this process is not precisely known. Fungal infection was not detected from the generalized skin lesions. Various clinical forms of dermatophytid reaction are discus

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01406.x

年代:1994

数据来源: WILEY

摘要:

AbstractAn 83‐year‐old woman had developed an asymptomatic, yellowish, dome‐shaped skin tumor on her scalp which had enlarged for a period of 60 years to 10 times 10 times 10 mm in size. Histopathologically, the tumor consisted of undifferentiated basaloid cells, differentiated sebaceous cells and transitional cells. Although the basaloid cells resembled those of basal cell epithelioma (BCE), the tumor was distinct from BCE in the following points; existence of cystic spaces, no peripheral palisading, no proliferation of connective tissue stroma, and no tendency toward local invasion. Immunohistochemical studies using antikeratin monoclonal antibodies revealed that the tumor contained both the keratin types of BCE and of sebaceous glands. Electron microscopically, the tumor cells contained lipid droplets and keratohyaline granules in their cytoplasm. It is suggested that “sebaceous epithelioma” is a benign skin tumor which may be distinguished cytologically

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01407.x

年代:1994

数据来源: WILEY

摘要:

AbstractAn unusual association of scrofuloderma of the scalp, psoas abscess, and caries spine was seen in a 4‐year‐old girl. The diagnosis was confirmed by relative and absolute criteria. The peripheral T‐lymphocytes and their subsets were within normal limits, although her immunohistopathological changes indicated an intermediary response across the spectrum depicting an unstable immunity. This may have been responsible for the silent spread ofMycobacterium tuberculosisand the current associ

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01408.x

年代:1994

数据来源: WILEY

摘要:

AbstractFebrile ulceronecrotic Mucha‐Habermann's disease (FUMH) was first described by Degos in 1966. In the literature, nine cases of FUMH have been reported in both children and adults. We report a 16‐year‐old boy with the febrile ulceronecrotic type. A review of the nine cases in the literature showed acute necrotic lesions, as well as rare complications such as fever, superinfected lesions and viral infection which are not as common in pityriasis lichenoides et varioliformis acuta. There is no definitive treatment, but systemic corticosteroid, methotrexate, antibiotics (tetracycline, erythromycin), aciclovir, and 4,4‐diaminodiphenyl sulfone (DDS) have been frequently used. The most common histologic feature is mononuclear perivascular infiltrates consisting of T lymphocytes. The etiology is not known, but a hypersensitivity reaction, possibly to an infectious agent, is su

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01409.x

年代:1994

数据来源: WILEY

摘要:

AbstractGeneralized argyria, the systemic dissemination and tissue deposition of silver‐containing particles, is characterized by slate gray discoloration of skin, most pronounced in sun exposed areas.A 33‐year‐old woman visited our dermatologic clinic complaining of frequent oral ulceration for 10 years and generalized discoloration of her skin for 5 years. She had had her tongue painted with silver nitrate repeatedly 6 years ago for the treatment of oral ulcers. Physical examination showed slate gray discoloration of her skin, most pronounced on the face and neck. The oral mucosa, tongue, sclera, and conjunctiva also had a slightly blue‐gray discoloration. Biopsy specimens from the oral mucosa and forearm revealed small brown‐black granules scattered in the dermis and basal lamina of eccrine sweat glands, blood vessels, and hair follicles under the light microscope. Tiny black granules were most numerous in the basal laminae of vessels in electron microscopic observation. Energy dispersive X‐ray microanalysis (EDXA) confirmed that many of the granules conta

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1994.tb01410.x

年代:1994

数据来源: WILEY