摘要:

AbstractIn FRSK cells, a cell line derived from fetal rat epidermal cells, cyclic AMP‐elevating agents forskolin (10 μM) and cholera toxin (10 ng/ml) increased cellular cyclic AMP content and suppressed [3H] thymidine incorporation. These effects of forskolin were enhanced by the phosphodiesterase inhibitor 3‐isobutyl‐***l‐methylxanthine (0.1 mM). Dibutyryl cyclic AMP (1 mM), an analog of cyclic AMP, decreased not only basal but also both tumor promoter 12‐O‐tetradecanoylphorbol‐13‐acetate***‐ and epidermal growth factor‐stimulated [3H] thymidine incorporation. From these results, we suggest that cyclic AMP may be a negative regulatory factor of DNA syn

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01211.x

年代:1989

数据来源: WILEY

摘要:

AbstractInduction of the transglutaminase activity of a transformed mouse epidermal cell line (PAM 212 cells) by 1α,25‐dihydroxyvitamin D3(1α,25‐(OH)2‐D3), the active form of vitamin D3, was investigated. Addition of 1α,25‐(OH)2‐D3to a culture medium stimulated twice the transglutaminase activity at a concentration of 10−7M, but vitamin D3, prostaglandin E1, E2, and F2afailed to show this induction. Phorbol 12‐o‐tetradecanoylphorbol‐13‐acetate (TPA) and ***dexamethasone also induced an increase in transglutaminase activity. Exposure to both 1α,25‐(OH)2‐D3and retinoic acid caused remarkably synergistic effects on the induction of transglutaminase in the PAM 212 cells. In contrast, simultaneous addition of 1α,25‐(OH)2‐D3and TPA was antagonistic and resulted in less than additive induction. Vitamin D3also showed a similar but lesser effect. These results suggest that 1α,25‐(OH)2‐D3induces the transglutaminase activityviamechanisms disparate from those of retinoic acid a

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01212.x

年代:1989

数据来源: WILEY

摘要:

AbstractIn newborn rat skin, disulfide bonds exist in the cell membrane of the stratum corneum and in the cytoplasm of the stratum granulosum. However, in the latter region, they can be detected after ethanol fixed epidermis remains for 2 weeks at 4°C. Thus, the idea arose that the visualization of the disulfide bonds in the stratum granulosum may be caused by proteases. Therefore, thein situeffects by both activation and inhibition of epidermal proteases were examined in tissue sections. Cryostat sections which were fixed in cold ethanol (—20°C, 98%) were incubated either in PBS, pH 7.4, containing 10mM 2‐ME as a control or in an epidermal homogenate (15,000×g supernatant fraction containing 2‐ME) at 37°C for 30 minutes and 4 hours, respectively, in order to activate the epidermal proteases and then reacted with NEM, 2‐ME, and DACM in steps. Many minute fluorescent particles were seen in the stratum granulosum under both of the above conditions. However, with incubation in PBS without 2‐ME, no fluorescent particles were seen. In addition, when the tissue was treated with NEM and zinc chloride before incubation, no fluorescent particles were seen. O‐phenanthrolin remarkably inhibited the appearance of these fluorescent particles. In contrast, leupeptin and antipain only slightly inhibited the appearance of these fluorescent particles, and pepstatin and phenylmethylsulfonyl fluoride didn't inhibit at all. In general, as NEM and zinc chloride strongly inhibit thiol proteases, these findings suggest that presence of the minute fluorescent particles in the stratum granulosum could be due to the effects of proteases, especially

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01213.x

年代:1989

数据来源: WILEY

摘要:

AbstractWe report 7 cases of acute fatal illness characterized by fever, diffuse erythematous rash, and progressive leukopenia occurring 10 days after surgical operation. The outcome was uniformly fatal. The biopsy findings consisted of eosinophilic individual necrosis of epidermal cells, satellite cell necrosis, basal liquefaction degeneration, and scanty cell infiltration into the dermis. T lymphocytes were found in the epidermis but Langerhans cells disappeared. These findings are compatible with acute graft‐vs‐host disease following blood transfusion. Explanations based upon drug allergy, infection, toxic shock syndrome, or toxic epidermal necrolysis seem less reasona

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01214.x

年代:1989

数据来源: WILEY

摘要:

AbstractWe have carried outin vitrostudies of hyperthermic effects on the viability, blastogenesis, and ultrastructural changes of atypical lymphocytes isolated from the peripheral blood of three patients with malignant lymphoma/leukemia. One was lymphocytic lymphoma/leukemia (T‐cell origin), and the others were adult T‐cell lymphoma/leukemia (both acute and smoldering type). In the cases of adult T‐cell lymphoma/leukemia, the peripheral lymphocytes were found to be more sensitive to hyperthermia than those of non‐ATL patients in terms of trypan‐blue exclusion ability and blastogenesis ability with concanavalin A challenge. When heated under sublethal conditions, lymphocytes of both ATL and non‐ATL patients exhibited the following characteristics: production of multilayered nuclear membranes, increase in vesicles (lysosomes), swelling of mitochondria, and cytoplasmic va

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01215.x

年代:1989

数据来源: WILEY

摘要:

AbstractThe collagens synthesized by Pam cells were biochemically characterized. The collagen in the cell layers was identified as type IV collagen by immunoprecipitation with type IV collagen specific antibody. The apparent molecular weights were 180 Kd and 175 Kd, which converted to major (160 Kd) and several minor smaller fragments with pepsin treatment. The ratios of 3‐hydroxyproline/4‐hydroxyproline and 4‐hydroxyproline/proline in the 160 Kd polypeptide were 0.11 and 1.18, respectively. The result shows that Pam cells are able to produce type IV collagen whose biochemical characteristics are similar to those from other epithelial cells.In the kinetic study, the synthesis of type IV collagen reached its maximum level rapidly on the 1st day after seeding, suggesting that type IV collagen may play a role in cell attachment and prolifer

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01216.x

年代:1989

数据来源: WILEY

摘要:

AbstractThe glycosaminoglycan (GAG) content of the extracellular matrix of burn scar in humans has been reported to differ from that of normal skin (1, 2). In order to investigate whether the GAG content altered as a result of functional changes in fibroblasts, the GAG content was determined in culture media of fibroblasts derived from growing burn scar, mature scar, and normal skin tissue. No statistical differences were observed in the population doubling‐times of scar and normal skin. Mature scar showed significantly higher values for all the concentrations of uronic acid, hexosamine, and sulfate measured in the glycosaminoglycan, as compared with normal skin values, and the concentrations from growing scar were slightly higher than those for normal skin. The above results may suggest an increase in glycosaminoglycan sulfate synthesis following the hyperplasia of the matrix in burn scar tissu

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01217.x

年代:1989

数据来源: WILEY

摘要:

AbstractUtilizing three different anti‐keratin antibodies and the avidin‐biotin peroxidase complex system on sections of formalin‐fixed paraffin‐embedded tissues and on cryostat sections, immunohistochemical localization of keratin type intermediate filaments in mammary Paget's disease and extramammary Paget's disease was investigated. Anti‐keratin antibodies EAB‐903 and EAB‐904, which recognize 66K and 57K dalton keratin peptides, did not decorate any Paget's cells in either mammary or extramammary Paget's disease. On the other hand, anti‐keratin antibody MAK‐6, which recognizes 52.5K, 50K, 48K, 45K and 40K daltons keratin peptides, did decorate Paget's cells in both Paget's diseases. These staining properties of Paget's cells were the same as those of secretory cells in normal human sweat glands and mammary glands. Anti‐keratin antibody MAK‐6 is thought to be useful in the diagnosis of mammary and extramam

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01218.x

年代:1989

数据来源: WILEY

摘要:

AbstractA female infant, at 6 weeks old, with the clinical manifestation of annular erythemas on the trunk and face, was positive for anti‐SS‐A and anti‐SS‐B antibodies. The annular erythema disappeared spontaneously at the age of 7 months when anti‐SS‐A and anti‐SS‐B antibodies were negative. Electron microscopic observation of the vascular endothelial cells on the annular erythema revealed microtubular structures. Later microtubular structures were found absent from the vascular endothelial cells of the area where the annular erythema had disappeared. The patient's mother is suffering from Sjögren's syndrome; she has no clinical symptoms but anti‐SS‐A and anti‐SS‐B antibodies are positive and a biopsy of small salivary glands of the lip demonstrated a marked periductal mononuclear cell infiltration. Microtubular structures were observed in her vascular endothelial cells in the small salivary gland region. These findings suggest that disappearance of microtubular structure may have some relationship with anti‐SS

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01219.x

年代:1989

数据来源: WILEY

摘要:

AbstractExacerbation of psoriasis was observed in a 28‐year‐old patient following the addition of lithium carbonate to a treatment regimen of carbamazepine and other drugs for ***manic‐depressive psychosis. The biopsied lesion histologically showed a typical psoriasiform tissue reaction and immunohistochemically, remarkable infiltration of activated helper T cells. Immunologic reaction to lithium in a patch test and a lymphocyte proliferation test could not be demonstrated. The mechanisms of lithium action and their relations to the exacerbation of psoriasis were reviewed. In this patient, although we could not define which effect of lithium was most important, it seemed that an increased blood lithium level combined with simultaneous carbamazepine administration enhanced the triggering effect of lithium and caused the exacerbation of psor

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1989.tb01220.x

年代:1989

数据来源: WILEY