摘要:

AbstractOmission of L‐glutamine (Gln) from the culture medium decreases tyrosinase activity and increasesγ‐GTP activity in cultured melanotic B‐16 melanoma cells; inclusion of this amino acid in the medium shows the reverse effects. Its absence, however, decreases theγ‐GTP activity in non‐pigmented cells like HeLa cells, fibroblasts and amelanotic melanoma cells and its presence induces a dose dependent increase in this enzyme activity in these cells. The decrease ofγ‐GTP activity in the melanotic melanoma cells by Gln is attributed to increased production of dopa and its derivatives by these cells, as evidenced by our results. Our data also indicate the inhibitory effects of dopa and several other dopa derivatives known to be produced by the melanoma cells on isolated bovi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03640.x

年代:1988

数据来源: WILEY

摘要:

Abstractα‐Aminoisobutyric acid transport has been demonstrated in cultured human epidermal keratinocytes as well as in the transformed state of these cells. The concentrative uptake is sodium‐dependent and may be ascribed to one Michaelis‐Menten component whose maximal velocity is 6.9 nmol/min × 106cells, with an apparent affinity of 3.8 mM. These parameters may be modified, depending upon the nature of malignant transformation. In SVK14 SV40‐virus‐transformed cells, there is no change of affinity but the maximal velocity is 1.5 fold less than in normal cells. In the spontaneous epidermoid carcinoma‐derived A431 line, this phenomenon is inverted; the maximal velocity is unmodified but the system affinity is 2.2‐fold higher than in normal cells. The unresponsiveness of this transport system to insulin and epidermal growth factor (EGF) shows that it behaves differently from those of many o

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03641.x

年代:1988

数据来源: WILEY

摘要:

AbstractCounterflow centrifugal elutriation (CCE) is one of the most powerful and rapid methods for separating free cells by sedimentation velocity based on cell size, density, or shape. To obtain a sufficient number of cells, the fractionation of guinea pig epidermal keratinocytes was developed with the use of a newly‐developed elutriation system. An EDTA‐trypsin method was used to isolate keratinocytes; they were then separated into three groups according to flow rate by CCE. Morphologically, the keratinocytes in each region were identified as basal cells, spinous cells, and granular cells. The DNA distribution pattern agreed well with that obtained by Percoll's density gradient. The light‐scatter of keratinocytes in each fraction obtained by the present method was similar to that obtained with the density gradient m

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03642.x

年代:1988

数据来源: WILEY

摘要:

AbstractA haematoxylin stainable protein (HSP) extracted from the EDTA‐separated epidermis of 3‐day‐old rats was purified through preparative isoelectric focusing followed by fast protein liquid chromatography (FPLC) using a Superose 12 column and a Mono‐Q anion exchange column. The purified protein was a single protein in SDS‐PAGE; it had a molecular weight of 14,500 daltons and an isoelectric point of pH 4.7. The amino acid composition included 14% glutamic acid, 13.7% lysine, 12% aspartic acid, 10% glycine, 8% valine and 0.3% ornithine, but only 1.2% histidine.An antibody elicited by the purified antigen also localized in the cell membrane region of the stratum corneum cells of the flank skin, but not in the keratohyalin granules, in indirect immunofluorescen

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03643.x

年代:1988

数据来源: WILEY

摘要:

AbstractThe distribution of 2,4‐dinitrophenyl (DNP) groups on Thy‐1 positive epidermal cellsin vivoafter 2,4‐dinitrochlorobenzene (DNCB) was painted on the skin surface of C3H/He mice was examined. Epidermal cell suspensions (EC) derived from trypsin‐separated mouse ear skin painted with DNCB were double‐stained for DNP groups and Thy‐1 protein using FITC‐anti‐DNP antibody, biotinylated anti‐mouse Thy‐1,2 monoclonal antibody, and phyco‐erythrin conjugated avidin. There were on the average 1.7% Thy‐1+ECs; DNP groups were detectable on 77.8% of them when the specimen was treated with anti‐DNP followed by anti‐Thy‐1,2 incubation. The incidence of Thy‐1,2+EC with DNP groups decreased when cells were treated with these antibodies in the reverse order. The significance of these findings is discussed in relationship to th

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03644.x

年代:1988

数据来源: WILEY

摘要:

AbstractSera of a 30‐year‐old Italian female and a 32‐year‐old Japanese female with prolidase deficiency were analysed by two‐dimensional immunoelectrophoresis, rocket immunoelectrophoresis, and single radial immunodiffusion (SRID). In both patients, amounts of transferrin, albumin and IgM were significantly low, while concentrations ofα1‐acid glycoprotein,α1‐antitrypsin,α1‐antichymotrypsin, ceruloplasmin, IgA and Clq were elevated in comparison with levels of these proteins in sera of healthy Italian and Japanese female adults (as normal controls). The amount of haptoglobin was conspicuously high in the Italian patient but remarkably low in th

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03645.x

年代:1988

数据来源: WILEY

摘要:

AbstractIn order to improve the oxidative stability of hydroquinone (HQ), the only depigmenting agent available clinically for skin, 14 hydroquinone fatty acid esters (HQ‐Rs) were synthesized and examined for their stability, efficacy, and intradermal metabolism. HQ‐Rs containing more than 3 carbon atoms in the fatty acid side chain showed excellent stability in an aqueous base (pH 6). HQ‐Rs containing fewer than 8 carbon atoms in the fatty acid side chain exhibited skin depigmenting potencies better than that of HQ. HQ‐R was quantitatively hydrolyzed by a skin homogenate to liberate HQ. The hydrolysis velocity of the HQ‐Rs was dependent on the length of the fatty acid side chain; the longer the length, the slower the hydrolysis velocity. From these results, we concluded that HQ‐R is absorbed into the skin after topical application, hydrolyzed by esterase, and it depigments the skin by liberating HQ. Thus, HQ‐R function as a prodrug of HQ; it exhibits both an excellent oxidative stability and an excellent skin depigmenting potency by liberating HQ wi

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03646.x

年代:1988

数据来源: WILEY

摘要:

AbstractSerum gastrin levels did not change during topical corticosteroid therapy with an experimental dose of 20 to 60 g/day for seven consecutive days, and serum 11‐hydroxycorticosteroid levels were significantly suppressed during the treatment. On the other hand, after daily oral administration of 1.5 mg/day betamethasone for seven consecutive days in volunteers who had fasted overnight, serum gastrin levels were higher and 11‐hydroxycorticosteroid levels were significantly lower.Fasting serum gastrin levels were significantly increased in rats after intraperitoneal administration of prednisolone at dosages of 2, 10, 20, or 50 mg/kg/day, but they were not significantly altered after the administration of prednisolone at 0.5 mg/kg/day. Serum gastrin levels were not significantly altered in man or rats after the administration of a histamine H1receptor antagonist. Therefore, serum gastrin levels are apparently not influenced by topical corticosteroid treatment although they are altered after systemic corticosteroid administrat

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03647.x

年代:1988

数据来源: WILEY

摘要:

AbstractFixed drug eruption incidence was 0.3% of outpatients. It affected individuals of both sexes in their 3rd and 4th decades, with a preponderance of males. Literary index and occupation were inconsequential. High index of suspicion for an etiologic drug was cardinal and was invariably supported by history. The clinical features of FDE were characteristic; it affected the upper and lower extremities, trunk, and lower lips. The diagnosis was confirmed by provocation test in 86 and cross‐sensitivity in 11 patients. Analgesics and chemotherapeutic agents were primarily responsible for FDE in this populatio

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03648.x

年代:1988

数据来源: WILEY

摘要:

AbstractA case of incompletely differentiated sebaceous carcinoma and its histopathological findings were reported. In addition, morphological criteria for separating pseudo‐sebaceous tumors into categories were discussed.In this case, histopathological findings showed a mixture of basaloid and squamoid features. Specimens were examined with both light and electron microscope

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1988.tb03649.x

年代:1988

数据来源: WILEY