摘要:

ABSTRACTExplant cultures were attempted from the so‐called adenoma sebaceum in the face and a plaque lesion on the neck observed in three genetically related cases (three males twelve, fifteen, and 42 years old) with Pringle's disease, tuberous sclerosis. Then the DNA content of outgrown non‐epithelial cells (NEC), or stroma cells, derived from the respective tissues (10,000 nuclei and 15,000 cells) were investigated by a flow cytofluorometer.1) DNA histograms per cell showed no remarkable differences between patients or lesions. On the other hand, 2) DNA histograms per nucleus did show certain differences. Among similar lesions, higher degrees of involvement showed more remarkable differences. 3) The NEC from the mulberry‐shaped tumor (adenoma sebaceum) showed obviously reduced peaks of 2C and 4C, and an increased width and increase in cells with their DNA content corresponding to the S‐phase. In addition, 4) there were no findings suggesting the presence of cells with excessive DNA content

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01432.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTAn important approach to the treatment of auto‐immune diseases is the selective removal of specifically pathogenic antibodies from the blood. This study reports the effectiveness of using DNA‐combined immuno‐absorbent matrixes for the removal of anti‐DNA antibodiesin vitro. Sera obtained from patients with Systemic Lupus Erythematosus (SLE) were used, as the pathogenesis of SLE is partially mediated by antibodies against DNA (1). A series of DNA‐combined immuno‐absorbent matrices was prepared using several different approaches, and the adsorption of anti‐DNA antibodies from the SLE sera was investigated on these matrices. A comparison was then made between these matrices and commercially available adsorbents with respect to their adsorbing abilities and their stability in sera. Readings of the following were also made before and after the reaction; pH, albumin, globulin, CH50, compliments C5, C4, C5, DNA, DNA and anti‐DNA antibodies complex ratios. Of the adsorbents investigated, the newly prepared immuno‐adsorbent matrix of DNA, glow discharge treated DNA‐PVA on cotton gauze, proved to be the most effective in reducing the anti‐DNA antibodies and immune complex levels specifically, while remaining relatively

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01433.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTThe distribution of DNP groups on epidermal single cells and epidermal sheets prepared from the skin of guinea pigs three hours after painting with 5% DNCB‐ethanol solution was examined by scanning immunoelectron microscopy using bacteriophage T4 as a visual marker. The study showed that DNP groups were distributed diffusely on the surface of epidermal cells, in particular keratinocytes, and suggests that DNCB may bind to the surface components of epidermal cells when painted on the ski

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01434.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTThe oral administration of synthetic broad spectrum protease inhibitor, [N,N‐dimethylcarbamoylmethyl 4‐(4‐guanidinobenzoyloxy)‐phenylacetate] methanesulfate, was used to challenge 3‐methylcholanthrene‐induced skin carcinoma in the rapid growing stage.This preparation was administered as a 0.1% addition in diet or in a dose of 75 mg/kg twice a day to 26 mice harboring solid tumors. This protease inhibitor prolonged the survival time in the cancer bearing mice.These results suggest that the oral administration of inhibitors of kinin‐forming proteases such as trypsin, plasmin, and kallikrein exerts an antitumor effect on mal

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01435.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTThe dose rate effect of ultraviolet light (UV) was investigated from a view point of the law of reciprocity bothin vivoandin vitro. The reciprocity in UV erythema reaction was examined in seven excision repair deficient xeroderma pigmentosum (XP) patients with monochromatic UV at wavelengths of 255 nm and 290 nm using a 1% neutral filter (NF) to reduce UV intensity to 1/100. For thein vitrostudy, the reciprocity in UV cell killing was examined in four normal and three excision repair deficient XP fibroblast strains using varied intensities to 1/100 at 254 nm.As in the results obtained by Satoh et al. in normal subjects, the law of reciprocity did not hold for erythema reaction in XP patients at 255 nm when the dose rate was changed a hundred fold. In the XP patients, the minimal erythema dose (MED) required by 1/100 intensity was on the average 1.57 times higher for 255 nm and 1.12 times higher for 290 nm as compared with MED without NF. On the other hand, the law of reciprocity was found to hold for cell killing in both normal and XP cells by 254 nm UV at the intensity range of 1:100.

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01436.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTTwelve fragrance materials were patch tested on patients with various facial dermatoses at three different concentrations to determine suitable concentrations of these materials for patch testing.The results indicate that 2% rose oil, 10% oil of bergamot, 5% jasmine absolute, 10% ylang ylang oil, 5% benzyl alcohol, 2% benzyl salicylate, 2% sandalwood oil, 2%αandβsantalol, 2% isobornyl cyclohexanol, 5% hydroxycitronellal, 5% eugenol and 1% trans‐isoeugenol in white petrolatum are the optimal concentrations for testing, showing good accord with those recommended by ICDRG except for jasmine absolute, ylang ylang oil, and trans‐isoeugenol, which should be retested at concentrations lower than the range used in the present

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01437.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTBased on several reviews in Japanese dermatological journals, seventy‐five cases of bacteriologically confirmedMycobacterium marinuminfection of the skin from 1969 through 1980 have been accumulated. These 75 cases accounted for 82% of all reported cases of atypical mycobacterial infections of the skin in Japan (1).The following features were noted: Approximately half of the cases were occupationally related to fish handling; the other half occurred among fish tank hobby enthusiasts. The clinical presentation usually suggested sporotrichosis, with the majority of the skin eruptions appearing on the hand and 96 % being unilateral. Minocycline is recommended as the drug of choic

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01438.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTKeratin and prekeratin were extracted from human plantar epidermis and carried on SDS‐PAGE. Prekeratin in the basal as well as spinous cell layer of the epidermis was composed of 6–7 bands (M.W.: about 71,000‐49,500), while keratin in the surface of stratum corneum was composed of 3–4 bands (M.W.: about 66,500–53,000), indicating molecular modulation of prekeratin during epidermal cell differentiation.Amino acid compositions of prekeratin and keratin were similar, glycine, glutamic acid and serine being major three amino acids. Since the keratin molecule is known to be composed of a repeating amino acid sequence [Fuchs&Green:Cell,15, 887–893 (1978)], our result indicates that the modulation of molecular weight of prekeratin during differentiation might be due to enzymatic splitting of peptides of these

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01439.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTIn order to determine the lowest concentration of griseofulvin (GF) needed to induce abnormal porphyrin metabolism, D‐D strain mice were fed with a feed containing GF in concentrations of 0.1%, 0.5%, and 1.0%. The liver and blood porphyrin levels were analyzed, and the red fluorescence of the liver and blood observed with a fluorescent microscope. In the 0.5% GF and 1.0% GF groups, a swelling of the liver was observed, and coproporphyrin and protoporphyrin levels in the liver and the blood increased markedly. However, the increase in protoporphyrin levels was more prominent than the increase of coproporphyrin levels. The increase in the liver protoporphyrin was more marked than that in the blood porphyrin. Comparisons of the 0.5% GF and 1.0% GF groups revealed that liver swelling was more prominent in the 1.0% GF group. A high degree of metabolic abnormality in blood protoporphyrin was found in 1.0% GF animals whose feeding period was rather short. In the 0.1% GF group, liver swelling was hardly noticeable, and there were no differences between the short feeding and long feeding groups. Although no abnormalities in blood porphyrins were noticed in comparison with the normal group, abnormally high levels of liver porphyrins were found in 3 out of the 34 treated mice. No differences from the normal group were noted in the remaining 31 animals. In the 0.5% GF and 1.0% GF groups, red fluorescence of the liver was seen in all cases, while in the 0.1% GF group, reticular red fluorescence was noted in only one animal.From these findings, it appears that a marked increase in porphyrin occurs at a concentration above 0.5% in D‐D strain mice, whereas, at the concentration of 0.1%, the majority of the treated mice remain within normal limits. Only a few showed any abnormality of porphyrin metabolism. We feel that, for this reason, it would be better to use a GF concentration of 0.1% for the lowest concentration experiments involving GF‐induced protoporphyria in D‐D strain mice and especially for investigations of the interaction of other chemicals with GF, and investigations of initial changes of porphyrin met

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01440.x

年代:1984

数据来源: WILEY

摘要:

ABSTRACTThree types of collagen were found in neurofibroma tumor tissue from a patient with neurofibromatosis (Von Recklinghausen's disease). Two of them were determined to be type I and type III collagen by CM‐cellulose chromatography and SDS‐polyacrylamide gel electrophoresis. The third matched a membrane type collagen from amnionic and chorionic membrane of human placenta in its elution profile during CM‐cellulose chromatography and its electrophoretic patterns on SDS‐polyacrylamide gel. This collagen was precipitated with 3.6‐4.0 M NaCl at neutral pH after limited pepsin digestion. It was composed of two components with molecular weights of approximately 110K and 125K daltons. These collagenous constituents had a high content of hydroxylysine linked disaccharide units (glucosylgalactose) and were resistant to reduction. They were digested by bacterial collagenase (EC 3.4.24.3) but resisted tadpole collagenase (EC

ISSN:0385-2407    

DOI:10.1111/j.1346-8138.1984.tb01441.x

年代:1984

数据来源: WILEY