摘要:

AbstractPrevious studies showed that the expression of catalase‐2 (CAT‐2) and other glyoxysomal proteins is independently controlled in the scutella of intact maize seedlings. In this study, removal of the embryonic axis prior to seed imbibition dramatically decreased the amounts of all but two of the 19 immunologically detectable glyoxysomal proteins in the scutellum, including CAT‐2. The temporal expression profile of CAT‐2 was also altered. Removal of the axis after seeds were fully imbibed (24 hr) had little effect on the subsequent pattern of expression of CAT‐2. The effect of axis removal was specific for glyoxysomal enzymes and caused relatively little change in the population of stainable scutellar proteins. In vitro translation studies and nucleic acid hybridization with a gene‐specific cloned probe (forCat2)revealed that the mRNA levels for glyoxysomal proteins were sharply lowered by axis removal. This study provides evidence that a signal may be released from the embryonic axis during imbibition, leading to the expression of a set of glyoxysomal enzymes by enhancing either the transcription of their genes or transcrip

ISSN:0192-253X    

DOI:10.1002/dvg.1020100102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractDevelopmental defects called phenocopies can be induced by heating Drosophila melanogaster pupae at specific developmental stages. The induction of the defects is thought to be a result of interference with gene expression at some level (Petersen and Mitchell, Dev Biol 1987; 121:335‐341, 1987). Here we look at protein turnover in developing 52‐hour wings and at the effect of heat on the proteolytic processing of three proteins that normally turn over rapidly. The effect of the heat treatment itself on the turnover of each protein is different. However, all of the proteins appear to be stabilized at 25°C during recovery from severe heat sh

ISSN:0192-253X    

DOI:10.1002/dvg.1020100103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractWe have standardized the map of chorion structural gene clusters inBombyx moristrain C108 by analyzing quantitative and qualitative chorion electrophoretic markers in recombinant progeny from four independent crosses. In all we assigned 22 markers to three gene clusters, representing about one‐third of the total number of chorion genes: 2 toCh 1, 9toCh 2, and 8 toCh 3.Three additional markers belong either toCh7 orCh 2.By referring to published chorion protein synthesis patterns, we show that the clusters are restricted in their developmental specificities:Ch 3appears to be an early locus, carrying all of the mapped early markers (4) and half of the early middles (3/6), whileCh 1andCh 2 carrypredominantly middle (4/5) and all late, Hc (6) markers, along with some early middle markers (3). We cite evidence to show thatCh 1andCh 2compose the left and right halves of a single gene cluster, which we formally designate asCh 1

ISSN:0192-253X    

DOI:10.1002/dvg.1020100104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractInDrosophila melanogasterthe yolk protein (YP) genes are normally expressed only in the fat body and follicular epithelium of adult females‐never in males or in larvae. We describe here a first step toward a genetic examination of the developmental controls that restrict the activity of the YP genes to adult female tissues. A YP1 promoter that contains the tissue‐, temporal‐, and sex‐specific controlling elements for expression was fused to the reporter gene, alcohol dehy‐drogenase (Adh). The gene fusion was transformed into an Adh‐deficient genotype. As assayed by a number of criteria, that the fusion gene is expressed in the same physiological manner as the endogenous yolk protein genes. The fusion gene's activity is modulated intransby a temperature‐sensitive allele of the sex determination gene,tra‐2.The Adh enzyme serves as a selectable marker and therefore these flies are suitable for use in genetic screens for trons‐acting mutations that affect the expression of the y

ISSN:0192-253X    

DOI:10.1002/dvg.1020100105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThis study isolated cDNA clones from egg‐chamber and adult female Drosophila cDNA libraries using as probe a DNA fragment from a 200‐kb “chromosome walk” in region 32E of the second chromosome of D. melanogaster. The present authors believe that these clones correspond to a new vitelline membrane protein (VMP) gene because (1) cDNA clones in Northern blots identify a transcript expressed in a tissue‐ and stage‐specific manner: stage 10 egg‐chambers; (2) the sequence of cDNAs and of the genomic subclone shows homology with the other VMP genes that have been identified to date; (3) the amino acid composition of the translational product has the high content of proline and alanine characteristic of VMPs. Two aspects emerging from this study are worth stressing: (1) the presence of a hydrophobic domain that is highly conserved in all the VMP genes; and (2) the particularly narrow period of expression of the isolated gene, which could be related to the mechanism of vitelline memb

ISSN:0192-253X    

DOI:10.1002/dvg.1020100106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThe construction of cell hairs on the wings in developing pupae of Drosophila provides a unique system for studies of the regulation of differentiation in the absence of cell division. Early steps in hair construction are the extrusion of cell hairs and the deposition of the external impervious layer called “cuticulin.” Some properties of six of the most abundant proteins that are present during the early stages of hair construction are described. These proteins make up about 40% of the total protein of the preparat

ISSN:0192-253X    

DOI:10.1002/dvg.1020100107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractTandTcare dominant mutations in the mouse that affect neuroaxial development when heterozygous and cause embryonic death when homozygous. Embryos were analyzed individually by two‐dimensional gel electrophoresis at 9½ days gestation, 1 day before homozygotes die in utero. A comparison of the protein patterns of mutant homozygotes with those of their littermates revealed a set of proteins (T‐proteins) that showed isoelectric point (pl) polymorphism. All the T‐proteins were more basic in mutant homozygotes. These polymorphisms could be detected, although they were less pronounced, in embryos as young as 7½‐day presomite stages, when it is impossible to distinguish homozygous mutants grossly. Interestingly, the same proteins show a pl shift from basic to acidic in wild‐type embryos during development from 7½ to 9½ days. Thus, it appears that inTandTcmutants a developmentally specific posttranslational acidic modification of these proteins is disturbed. The likely cause of the abnormality is a defect in some mechanism for phosphorylation, since the T‐proteins of wild‐type embryos were shifted to higher pls by phosphatase treatment. This disturbance appears to be localized to axial structures (neural tube, somites, and surrounding mesenchyme) since only these structures, and not the rest of the mutant homozygous embryos, contain abnormally

ISSN:0192-253X    

DOI:10.1002/dvg.1020100108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

ISSN:0192-253X    

DOI:10.1002/dvg.1020100101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY