摘要:

The urediospores ofMelampsora lini(Ehrenb.) Lev. are echinulate, with spines ca. 1 μ long over their surface. The spines are electron-transparent, conical projections, with their basal portion embedded in the electron-dense spore wall. The entire spore, including the spines, is covered by a wrinkled pellicle ca. 150–200 Å thick. The spore wall consists of three recognizable layers in addition to the pellicle. Spines form initially as small deposits at the inner surface of the spore wall adjacent to the plasma membrane. Endoplasmic reticulum occurs close to the plasma membrane in localized areas near the base of spines. During development, the spore wall thickens, and the spines increase in size. Centripetal growth of the wall encases the spines in the wall material. The spines progressively assume a more external position in the spore wall and finally reside at the outer surface of the wall. A mutant strain with finely verrucose spores was compared to the wild type. The warts on the surface of the mutant spores are rounded, electron-dense structures ca. 0.2–0.4 μ high, in contrast to spines of the wild type. Their initiation near the inner surface of the spore wall and their eventual placement on the outer surface of the spore are similar to that of spines. The wall is thinner in mutant spores than in wild-type spores.

ISSN:0008-4026    

DOI:10.1139/b71-291

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

A study of the vesicular and tubular bodies observed in cells ofPoria monticolawas done with particular regard to their form, origin, and membrane structure. The membranes of lomasomes and pinocytotic vesicles display the sometimes asymmetric, distinctly trilaminar structure characteristic of the plasma membrane. Lomasomes are abundant and vary in appearance depending upon the method of fixation used. Nonlamellar associations of vesicles and short tubules probably represent the true structure of lomasomes in this organism. These lomasomes originate from the plasma membrane and do not appear to be associated exclusively with wall formation.

ISSN:0008-4026    

DOI:10.1139/b71-292

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

The effect of exogenous substrates on the endogenous respiration of intact mycelium of the snow mold fungusTyphula idahoensisRemsb. was studied by manometric and isotopic techniques. Endogenous respiration was stimulated by glucose at 5 °C and 20 °C and by acetate at 5 °C. Stimulation by glucose tended to be greater at 5 °C than 20 °C in unstarved and 24-h-starved medium. The extent of stimulation increased at both temperatures after longer periods of starvation.Glucose oxidation also increased after starvation, indicating that it was normally suppressed by high levels of endogenous reserves. Between 5 °C and 20 °C, exogenous respiration of glucose and acetate increased three- and four-fold respectively while endogenous respiration only doubled. Oxidative assimilation of glucose at 5 °C and 20 °C and acetate at 5 °C was extremely high; lower amounts of acetate were assimilated at 20 °C. Comparison of14C distribution with and without the use of acid to terminate experiments indicated that considerable14C material was released from the mycelium upon acidification.The results are discussed with relation to the possible existence of separate pools of endogenous reserves and the thermosensitivity of some aspects of endogenous metabolism.

ISSN:0008-4026    

DOI:10.1139/b71-293

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

The F2progenies of four crosses, including one reciprocal, betweenPuccinia graminis triticiandP. graminis secalisincluded only cultures that were less virulent on wheat and rye than the parent that attacked one or other of these hosts. They closely resembled the F1culture from which they were derived. A number of F2cultures had low virulence on both wheat and rye. It is hypothesized that the hybrids resemble a more primitive form ofPuccinia graminisand that evolution in the stem rusts of cereals is progressing from low virulence and wide host range to high virulence and reduced host range.

ISSN:0008-4026    

DOI:10.1139/b71-294

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

Vanillic and salicylic acids, and the coumarins marmesin, herniarin, esculetin, and bergapten were identified as constituents of whole tomato plants by paper and thin-layer chromatography and ultraviolet spectroscopy in the presence of diagnostic reagents. Strong evidence for the occurrence ofp-hydroxybenzoic, syringic, ferulic, andp-hydroxycinnamic acids was obtained on the basis of chromatographic data and chromogenic reactions. Only the last two compounds and salicylic acid had been reported to occur in tomato plants. Biosynthetic implications of the occurrence of these phenolics are considered.

ISSN:0008-4026    

DOI:10.1139/b71-295

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

Within 1 week after inoculation of young tomato plants withAgrobacterium tumefaciensmarked qualitative differences from wounded controls were observed in the pattern of carboxylic acids liberated by acid hydrolysis of whole-plant extracts. Salicylic and gentisic acids were detected in the controls but not in the infected plants. In contrast,p-hydroxybenzoic acid and an unidentified acid giving a chromogenic reaction with diazotized sulfanilic acid were found only in the diseased plants. No qualitative differences persisted 2 weeks from inoculation, but beyond the first week salicylic and gentisic acids had not only appeared in the infected plants but the level of the latter considerably exceeded that in the controls. The syringic acid content of the diseased plants appeared to be higher throughout the 4-week duration of the experiment.

ISSN:0008-4026    

DOI:10.1139/b71-296

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

Bean seeds (Phaseolus vulgaris) yielded a soluble glycoprotein that accounted for about 35% of the total protein as determined by combined analysis with DEAE-cellulose and disc electrophoresis. Germination for up to 114 h had little effect on this glycoprotein or on the total soluble protein. The glycoprotein had an apparent molecular weight of 130 000 (6.1 S), contained 14.7% nitrogen, and yielded mannose, glucosamine, and some pentose upon hydrolysis.

ISSN:0008-4026    

DOI:10.1139/b71-297

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

Seeds of 10 species of dicotyledons were placed into varying concentrations of solutions of aniline (C6H5NH2) for 16 h. All three parameters studied, germination, growth, and pigmentation, were adversely affected in runner bean,Phaseolus coccineus; bush bean,P. vulgaris; mung bean,P. aureus; buckwheat,Fagopyrum esculentum; radish,Raphanus sativus; flax,Linum usitatissimum; eggplant,Solanum melongena; and tomato,Lycopersicon esculentum, by imbibition of a 0.05 Msolution. By contrast, pigmentation in sweet basil,Ocimum Basilicum, and sensitive plant,Mimosa pudica, was enhanced. Competition by aniline in hydroxylase reactions and in sugar-requiring processes via a postulated glycosylation detoxication reaction is suggested as a possible mode of action.

ISSN:0008-4026    

DOI:10.1139/b71-298

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

A study of the presumed type specimens ofCylindrosporium crassipesPr.,Cylindrosporium inflatipesPr., andCylindrosporium longipesPr. has shown that their transfer toChalaraby previous authors is justified.Chalara inflatipes(Pr.) Sacc. is an earlier name forChalara insigne(Sacc., Rouss. & Bomm.) Hughes. A collection in B (Botanisches Museum, Berlin-Dahlem, Germany) asChalara longipesforms the basis for a new species.

ISSN:0008-4026    

DOI:10.1139/b71-299

出版商:NRC Research Press    

年代:1971

数据来源: NRC

摘要:

Cronartium comandraePk. aeciospores were collected daily from individual cankers and pustules onPinus contortaDougl. var.latifoliaEngelm. Germination was high for the initial 2 to 4 weeks, but much lower during the second half of the sporulation period. Most individual cankers and pustules showed similar trends in their daily spore germination. Germination and weather conditions had no consistent relationship. However, wet spores rapidly lost color and germinated poorly. Contamination from the associated microflora, especiallyPenicilliumspp.,Cladosporiumspp., and bacteria, increased as the season progressed, and contributed to the reduced spore viability.

ISSN:0008-4026    

DOI:10.1139/b71-300

出版商:NRC Research Press    

年代:1971

数据来源: NRC