摘要:

AbstractIt was with great sadness that we received news of the sudden death on 29 August 1990 of Professor Toshio Goto who was a member of the Editorial Board of this Journal.

ISSN:0884-3996    

DOI:10.1002/bio.1170060102

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe effect of interleukin 2 (IL 2) on the capability of human monocytes to secrete reactive oxygen species triggered via Fc‐γ receptor (Fc‐γ R) function had been investigated by measurement of chemiluminescence (CL). IL 2 did not activate highly purified (hp) monocytes to respond to Fc‐γ R mediated phagocytic stimulation with an enhanced respiratory burst activity unless low numbers of T cells had been co‐cultured with hp monocytes. Supernatants from IL 2 treated PBMC contained interferon‐γ (IFN‐γ) and monocyte activating factor (MAF) activity. The secretion of both cytokine activities was strongly enhanced by cooperative function of monocytes. The correlation of IL 2 induced secretion of IFN‐γ and MAF activity was striking, however, monoclonal antibody (mAb) anti‐human IFN‐γ failed to abrogate IL 2 stimulated and lymphocyte dependent monocyte activation. Although IL 2 had no direct monocyte activating effect, pretreatment of hp monocytes with IL 2 led to monocyte priming: subsequent co‐culture with autologous control T cells enhanced the monocyte Fc‐γ R mediated CL response. The priming of monocytes by IL 2 was dependent on the interaction of IL 2 with the monocytic IL 2 receptor as shown by inhibition experiments with anti IL 2 R monoclonal antibody. Thus the IL 2 driven monocyte/T‐cell interaction leads to an increased Fc‐γ R mediated monocytic respiratory burst activity and to the secretion of a soluble MAF activity, but there w

ISSN:0884-3996    

DOI:10.1002/bio.1170060103

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractQuenching effects of purine and pyrimidine derivatives on the extra‐weak chemiluminescence (CL) derived from the Maillard reaction of L‐lysine with D‐arabinose were investigated The pyrimidine derivatives 2′‐deoxy cytidine, uridine, and uracil quenched the CL. Cytidine did not quench the CL. Purine derivatives, e.g. uric acid and 1‐methyl adenosine were particularly effective in quenching the CL. 5‐Methyl adenine and xanthine also quenched the CL, but adenosine had no effect. A comparison of the CL‐quenching abilities of compounds that have common basic structure was made; those with ribose at the 5‐position were the strongest quenchers. A linear relationship between CL‐quenching activity and the HOMO energy of the pi orbital for the various

ISSN:0884-3996    

DOI:10.1002/bio.1170060104

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractLight emission fromPhotobacterium phosphoreumwas analysed during cell growth on an agar plate from a single cell to colony formation. Temporal analysis of image intensified light was set so that a quadratic window covered a single cell. Intensity of light emission from a single cell through colony formation showed an initial decrease, a prolonged lag phase, and then a rapid increase. These responses on an agar plate were similar to those from liquid cultures. The image analysis showed repeated bursts of light emission in the phases when light was increasing and decreasing. Statistical analysis of light emission also emphasized the presence of bursts of light emission, suggesting the metabolic synchronism of luciferase reactions in either a single cell or synchronously divided cells. The repetitive bursts of light occurred in a single cell and continued during the growth phase in which the cell population and the light emission was increasing. In a single cell, however, periodicity of light emission was not defined directly from fast Fourier transformation, although it was indicated on oscillation of mean level of fluctuated light emission, at initial phase of culture on agar plate.

ISSN:0884-3996    

DOI:10.1002/bio.1170060105

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractUsing cultured human umbilical cord vein endothelial cells and human blood neutrophils, the interaction between neutrophils and endothelial cells,in vitro, was studied. The aim of the study was to examine whether a respiratory burst stimulation by neutrophils would be observed by neutrophil/endothelial cell interaction and whether the respiratory burst stimulation of neutrophils by endothelial cells could be enhanced by lipopolysaccharide stimulation of neutrophils. The second aim was whether such an effect, or secretion of elastase, could cause an endothelial cell damagein vitro.Chemiluminescence as an indicator of oxygen‐derived metabolites produced by neutrophils, elastase release by neutrophils, and endothelial cell damage, based on111In‐oxine release from labelled endothelial cells, were measured simultaneously. The present investigation demonstrates that neutrophils can be directly stimulated by endothelial cells. A further amplification of this process following lipopolysaccharide priming up to 10 ng/ml blood could be demonstrated. A slight endothelial cell damage occurs following neutrophil stimulation, although elastase secretion does not increase during interaction between neutrophils and endothelial cells. These results raise the possibility that oxygen‐derived metabolites rather than elastase contribute to an endothelial cell damage which might occur in conditions such as endotoxin‐induced adult respiratory distress s

ISSN:0884-3996    

DOI:10.1002/bio.1170060106

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractWhen polymorphonuclear leukocytes (PMNL) interact with the soluble stimulus formylmethionyl‐leucyl‐phenylalanine (FMLP), the cells increase their production of oxidative metabolites. This increased production can be measured as lumino‐amplified light emission or chemiluminescence (CL). In the present report, experimental systems which allow a quantitation of extracellularly and intracellularly generated metabolites have been used, and the effect of mannitol, benzoate, taurine, indomethacin and nordihydroguaiaretic acid has been investigated. The presence of the hypochlorous acid scavenger taurine had no effect on the intracellular response, whereas the extracellular response was reduced with around 50%. The hydroxyl radical scavenger mannitol had only minor effects on the response, whereas benzoate, another hydroxyl radical scavenger, reduced the extracellular response with around 50% and the intracellular response with more than 90%. Indomethacin, an inhibitor of arachidonic acid metabolism, did not influence the response, whereas NDGA, also an inhibitor of the arachidonic acid metabolism, totally abolished both the extracellular and the intracellular response. The use of scavengers/inhibitors as a means of determining the mechanisms of light emission, and the origin of chemiluminescence produced by neutrophils stimulated by FMLP is disc

ISSN:0884-3996    

DOI:10.1002/bio.1170060107

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe chemiluminescent reaction of an acridinium ester (AE) requires addition of peroxide to the 9 position of the acridinium ring. The addition of a hydroxide ion to the 9 position of an acridinium ester to form the carbinol adduct has also been well documented. We have observed a similar addition of other nucleophiles to the acridinium ring to form an acridan adduct. The adduct formed with bisulphite has been particularly well‐characterized for rate of formation, rate of reversion, and reaction equilibrium. The formation of an adduct (other than H2O2) has been demonstrated to decrease significantly the reactivity of the adjacent ester bond to alkaline hydrolysis. The resulting, more stable adduct is very useful when the acridinium ester is used as a label in DNA probe‐based assays. The adduct is highly resistant to hydrolysis under the conditions often desired for DNA probe‐based assays (high temperature, elevated pH, extended sto

ISSN:0884-3996    

DOI:10.1002/bio.1170060108

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170060109

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170060101

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY