ISSN:0884-3996    

DOI:10.1002/bio.1170010102

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractThe bioluminescent assay of ATP is rapid and simple and may be used as an estimate of microbial numbers. It therefore shows great potential as a technique to provied information on the microbiological quality of a food within a few minutes, in comparison with conventional techniques, which provide results retrospectively. Howver, despite the advantages of speed and senstitivity, no food microbiologists are using the technique for routine quality control and hygiene monitoring. This review seeks to highlight the reasons for this, and to offer some ideas for future research to increase the acceptance of ATP assays within the food industry.

ISSN:0884-3996    

DOI:10.1002/bio.1170010103

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractAn enzymatic assay ofD‐3‐hydroxybutyrate in which the hydroxybutyrate dehydrogenase reaction is coupled to the bacterial oxidoreductase—uciferase system is described. The bioluminescent assay is based on either, end‐point, or on initial velocity measurements. This simple and rapid assay requires a single serum sample of 10 μl. Its linear range covers two orders of magnitude from 10−6mol/I upwards. This assay is suitable for the routine determination ofD‐3‐hydroxybutyrate in human blood with

ISSN:0884-3996    

DOI:10.1002/bio.1170010104

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractOptimum conditions were established for the generation and measurement of luminoldependent chemiluminescence (CL) in human polymorphonuclear leucocytes (PMNL) stimulated with a variety of particulate and soluble agents. Several factors had a particular influence on the kinetics of CL stimulated by the chemotactic peptideN‐formyl‐L‐methionyl‐L‐leucyl‐L‐phenylalanine (fMLP). Two peaks, both azide‐sensitive, were observed at 21°C and 25°C. but these increased in magnitude and merged t o give a single, early peak when the temperature was increased t o 37°C. Pre‐exposure of PMNL to a buffer containing calcium was essential for the expression of both phases of fMLP‐stimulated CL, while the second peak decreased dramatically if the cells were stored at 4°C for 4 hours before assay. In contrast, storage of PMNL at 4°C for up t o 8 hours in a buffer without divalent cations did not alter the kinetics or magnitude of CL induced by other stimuli, and had the benefit of minimizing the rate of cell aggregation. This study confirms that measurement of luminol‐dependent CL in stimulated PMNL is a useful analytical tool, but shows that careful attention t o experimental design is required t o ensure that the observed CL provides a true measure of the par

ISSN:0884-3996    

DOI:10.1002/bio.1170010105

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractA conventional colorimetric peroxidase end‐point (ortho‐phenylenediamine substrate), used in an enzyme immunoassay for carcinoembryonic antigen, employing plastic beads as solid support, has been replaced by a much faster (30 seconds versus 30 minutes) enhanced chemiluminescent assay for the peroxidase label.Para‐iodophenol was used to enhance the light emission from the peroxidase catalysed chemiluminescent reaction between luminol and hydrogen peroxide. Values for precision and carcinoembryonic antigen concentration obtained with the chemiluminescent and colorimetric versions of the immunoassay on 62 serum specimens were in good agre

ISSN:0884-3996    

DOI:10.1002/bio.1170010106

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170010107

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170010109

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170010110

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0884-3996    

DOI:10.1002/bio.1170010101

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY