ISSN:0887-8013    

DOI:10.1002/jcla.1860010102

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractThe advances in medical technology in recent years has brought highly progressive developments into diagnostic microbiology. Therefore, it is not feasible to enumerate all the recent advances in clinical microbiology; attempts are made here to discuss only those developments of clinical relevance.The two major aspects of advances in microbiology are the discovery or isolation of new pathogens and the breakthrough in new technology. Of equal importance are some known pathogens which have taken on new roles either as the cause of a new clinical syndrome or as the leading cause of a known clinical syndrome.

ISSN:0887-8013    

DOI:10.1002/jcla.1860010103

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractThis review encompasses all aspects of recent C‐Reactive protein studies. Emphasis has been in the areas of (1) the physical, chemical, and potential functions of C‐Reactive protein; and (2) the pass and recent procedures of C‐Reactive protein analysis relative to simplicity and sensitivity. The responses of C‐Reactive protein in various diseases and its future prospects are also discussed in this

ISSN:0887-8013    

DOI:10.1002/jcla.1860010104

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractSequences of oncogenes define related families and provide a large data bank for the production of synthetic peptides. These peptides can be used to produce site‐directed monoclonal antibody probes, which identify oncogene‐encoded and oncogene‐related proteins. The creation and utilization of these probes for clinical laboratory analysis are disc

ISSN:0887-8013    

DOI:10.1002/jcla.1860010105

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractGene diagnosis is defined and evaluated in light of existing methods of clinical diagnosis. Gene diagnosis for diseases caused by somatic or inherited mutations is more powerful than existing clinical methods, but the state of this art is too cumbersome and expensive for routine diagnosis. Gene diagnosis for infectious disease is more specific than existing detection methods, but usually is not yet as sensitive as culture or as advanced. The format for both forms of gene diagnosis will evolve to liquid hybridization followed by a “dot” format for evaluating resu

ISSN:0887-8013    

DOI:10.1002/jcla.1860010106

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractA novel technique employed in preparing slides used for immunofluorescence staining of human tumor antigens with human anti‐cancer monoclonal antibodies is described. The anchorage of the cancer cells to the plastic substratum is used as a basis in eliminating cell fixation by physical and chemical procedures. In this method, the bottoms of the tissue culture flasks with the adherent A431 cell line, a carcinoma of the vulva, were cut into slides of 2 × 6 cm. This antigen‐positive target cell was then reacted with the VLN3G2 monoclonal IgG antibody and with the fluorescein‐labeled F(ab′)2of goat anti‐human IgG. Most A431 cells demonstrated intense fluorescence staining while others exhibited diffuse and often discontinuous membrane staining with the antibodies. In summary, this simplistic technique can be applied for the rapid detection of antigens displayed by the adherent tumor

ISSN:0887-8013    

DOI:10.1002/jcla.1860010107

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractThe identification and quantitation of intracytoplasmic immunoglobulins has become a useful tool in the clinical laboratory evaluation of neoplastic cells from patients with lymphocytic leukemia. Currently, the vast majority of these studies are made by fluorescent microscopy using cytocentrifuge preparations. In the present communication we report a new procedure for rapid flow cytofluorometric analysis of intra‐cytoplasmic immunoglobulin. This technique, utilizing a primary fixation step, permits maximal penetration of labeled antibody while preserving plasma membrane integrity and high retention of intracellular target proteins. These attributes contribute to a rapid yet accurate procedure for quantitating intracellular immunoglobulins which is potentially superior to any method currently utilized. Although the emphasis of this investigation has been on the labeling of intra‐cytoplasmic μ chain, continuing studies in our laboratory indicate that the technique provides a sensitive method for detecting a variety of intracellular anti

ISSN:0887-8013    

DOI:10.1002/jcla.1860010108

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractThe murine subrenal capsule assay can be used to estimate the susceptibility of fresh human tumor explants to various antineoplastic agents. As a result, the assay has been proposed as a rapid and accurate preclinical laboratory evaluation to determine the sensitivity of human tumors to chemotherapeutic drugs and thereby to assist the direction of cancer patient treatment. The subrenal capsule assay is described in detail and the predictive accuracy of the assay in the clinical setting is discussed.

ISSN:0887-8013    

DOI:10.1002/jcla.1860010109

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractTumors from 30 patients with advanced gynecologic malignancies were evaluated for sensitivity or resistance to various chemotherapeutic agents by three laboratory assays: the subrenal capsule (SRC) assay, the stem cell clonogenic (SCC) assay, and a delayed [3H]TdR incorporation ([3H]TdR) assay. The tumors were tested against a standard battery of chemotherapeutic drugs; 39% of the tumors were sensitive to one or more of these agents in the SRC assay compared to only 24% in the [3H]TdR assay and 4% in the SCC assay. Eighteen of the patients had adequate follow‐up to correlate their clinical course to the assay predictions. In this study, the prospective predictive accuracy (sensitivity) of the SRC assay was 100% compared to 25% for the [3H]TdR assay and 0% for the SCC assay. The respective specificities were 63, 73, and 80%. The SRC assay appeared to predict clinical response for gynecologic malignancies more accurately than either the [3H]TdR or the SCC assa

ISSN:0887-8013    

DOI:10.1002/jcla.1860010110

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY

摘要:

AbstractLymphoblastoid T cell lines from patients with Chediak‐Higashi syndrome (CHS) have been established by stimulating lymphocytes with phytohemagglutinin and interleukin‐2. This culture technique produced T cells containing giant granules which are characteristically seen in peripheral blood leukocytes of children with the disease. The granules were lysosomes as demonstrated by their reactivity with acid phosphatase. Functionally, they exhibited normal natural killer cell activity and/or lectin‐induced cytotoxicity. Variations in function of freshly obtained and long‐term cultured T cells are discussed. These cell lines will permit further studies to elucidate defects in lymphoid cells derived from patients w

ISSN:0887-8013    

DOI:10.1002/jcla.1860010111

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1987

数据来源: WILEY