ISSN:0167-6865    

DOI:10.1159/000179195

出版商:S. Karger AG    

年代:1996

数据来源: Karger

摘要:

We recently reported that the subcutaneous (s.c.) administration of a low-molecular-weight heparin (LMWH) fraction significantly inhibited de novo angiogenesis in the mesentery induced by the intraperitoneal (i.p.) injection of saline to adult rats compared with unfractionated heparin and high-molecular-weight heparin (HMWH) fractions. The present study assesses the effect on basic fibroblast growth factor (bFGF)-mediated de novo angiogenesis in the mesentery of the systemic administration of a LMWH fraction (2.6 kD) and a series of four HMWH fractions (about 20 kD) with varying degrees of polydispersity, charge density and anticoagulant activity. bFGF, a prototypic heparin-binding angiogenic growth factor, was injected i.p. at 220 pM on days 0-4. The heparins were given s.c. on days 0-13 or 0-14 at doses which were approximately within the range used clinically. Angiogenesis was assessed by microscopic morphometry and image analysis in groups of animals killed on days 14 and 15. Compared with the saline control, the LMWH and three fo the HMWHs significantly inhibited angiogenesis in terms of microvascular length (MVL), a measure of microvascular density. Interestingly, the vascularized area (VA), a measure of microvascular spatial extension, and the total microvascular length (VA × MVL) were significantly lower in the LMWH-treated animals than in the animals treated with one of the HMWHs. The total microvascular length was, moreover, significantly reduced in the LMWH-treated animals compared with the combined data of all the HMWH-treated animals. No significant effects were related to the degree of charge density and anticoagulant activity of the heparins. In view of the putative significant angiogenic role of bFGF in human angiogenesis diseases, the present findings may have implications for the choice of anticoagulant treatment modality for patients suffering from cancer and other angiogenesis diseases

ISSN:0167-6865    

DOI:10.1159/000179145

出版商:S. Karger AG    

年代:1996

数据来源: Karger

ISSN:0167-6865    

DOI:10.1159/000317401

出版商:S. Karger AG    

年代:1996

数据来源: Karger

摘要:

The reliability of intravital capillaroscopy for determining capillary density (CD) of skin has been questioned because it depends upon the variability of the measuring process and subjective interpretation of data as well as the intrinsic heterogeneity of capillary spacing. The aim of this study was to assess the repeatability of a standardised method for measuring CD of the skin of the dorsum of foot. In each of 30 subjects (10 controls and 20 patients with peripheral vascular disease), the foot was systematically mapped by examining 20 sites on the dorsum of foot and 2 sites on each toe, using white light (native) videomicroscopy at 40 × magnification. Off-line analysis of videoprints was then undertaken to determine CD at each site, by counting capillaries within areas of acceptable photographic quality only, having first defined the criteria for counting capillaries. The mean values were then calculated and taken to represent the CD of the foot or toes. Repeatability of the measuring equipment was first assessed by noting the presence or absence of each corresponding capillary in 2 prints, taken at intervals of hours or days (in 10 subjects) or months (in 2 patients), of an identical area of skin which was marked by a microtattoo on the first occasion. On average, 95% of corresponding capillaries were identified in both prints (from controls and patients), thus implying little intrinsic temporal variation of capillary anatomy as well as excellent repeatability of the measuring equipment. Repeatability of data analysis was assessed by the same observer reading the same 20 prints in a blinded manner on three separate occasions (intraobserver repeatability), and 2 observers reading the same 24 prints (interobserver repeatability). The mean coefficient of intraobserver variation of CD estimate was 5.6% and the interobserver correlation coefficient was 0.94. Finally, overall repeatability of the method was assessed by repeating the procedure on a subsequent occasion (mean time interval of 5 days) in 10 subjects. The rate of agreement in mean CD between the two procedures [defined as 100- (difference between the two measurements/mean of the two measurements) × 100]% ranged from 86.4 to 97.1% (mean 93.5%). Thus using the above methodological technique, native capillaroscopy can be reliably used to determine CD of the dorsum of foot in comparing patient subgroups, as well as in longitudinal studie

ISSN:0167-6865    

DOI:10.1159/000179147

出版商:S. Karger AG    

年代:1996

数据来源: Karger

ISSN:0167-6865    

DOI:10.1159/000179148

出版商:S. Karger AG    

年代:1996

数据来源: Karger

ISSN:0167-6865    

DOI:10.1159/000317402

出版商:S. Karger AG    

年代:1996

数据来源: Karger

ISSN:0167-6865    

DOI:10.1159/000317403

出版商:S. Karger AG    

年代:1996

数据来源: Karger

ISSN:0167-6865    

DOI:10.1159/000179150

出版商:S. Karger AG    

年代:1996

数据来源: Karger

ISSN:0167-6865    

DOI:10.1159/000317404

出版商:S. Karger AG    

年代:1996

数据来源: Karger

ISSN:0167-6865    

DOI:10.1159/000317405

出版商:S. Karger AG    

年代:1996

数据来源: Karger