ISSN:1075-2617    

DOI:10.1002/(SICI)1099-1387(199901)5:1<1::AID-PSC193>3.0.CO;2-F

出版商:John Wiley&Sons, Ltd.    

年代:1999

数据来源: WILEY

ISSN:1075-2617    

DOI:10.1002/(SICI)1099-1387(199901)5:1<3::AID-PSC194>3.0.CO;2-6

出版商:John Wiley&Sons, Ltd.    

年代:1999

数据来源: WILEY

摘要:

AbstractRecent progress in peptide and glycopeptide chemistry make the preparation of peptide and glycopeptide dendrimers of acceptable purity, with designed structural and immunochemical properties reliable. New methodologies using unprotected peptide building blocks have been developed to further increase possibilities of their design and improve their preparation and separation. Sophisticated design of peptide and glycopeptide dendrimers has led to their use as antigens and immunogens, for serodiagnosis and other biochemical uses including drug delivery. Dendrimers bearing peptide with predetermined secondary structures are useful tools in proteinde novodesign. This article covers synthesis and applications of multiple antigen peptides (MAPs), multiple antigen glycopeptides (MAGs), multiple antigen peptides based on sequential oligopeptide carriers (MAP‐SOCs), glycodendrimers and template‐assembled synthetic proteins (TASPs). Part I deals with the development of various structural forms of MAPs as well as their application as antigens, immunogens, and for immunodiagnostic and biochemical purposes. Copyright © 1999 European Peptide Society and John Wiley&Sons,

ISSN:1075-2617    

DOI:10.1002/(SICI)1099-1387(199901)5:1<5::AID-PSC178>3.0.CO;2-R

出版商:John Wiley&Sons, Ltd.    

年代:1999

数据来源: WILEY

摘要:

AbstractMessenger RNA purified from the anti hemin monoclonal antibody (1D3) secreting hybridoma was amplified by RT‐PCR and the nueleotide and amino acid sequences of the antibody were determined. The role of complementarity determining regions (CDRs) in porphyrin recognition and its immunochemical feature of the antibody were investigated by using ELISA, fluorescence measurement and computational calculation of the conformation. All CDR peptides of the heavy chain of the antibody were synthesized and their affinity constants to porphyrins were determined. The value of CDR2 of heavy chain (CDRH2) of 1D3 was 1.5×105/M for protoporphyrin and 7×107/M for TCPP, respectively, while that of the whole antibody showed to be 1.2×107/M for TCPP. Though CDRH2 is a 17 meric peptide, it showed higher affinity than the whole antibody (1D3). Porphyrins can be considered to firmly bind with CDRH2, while CDRH3 is not involved in the antigen binding. CDR‐1 may participate in the recognition with a small contribution. By the computational analysis of steric conformation, it was suggested that CDRH1 and CDRH2 co‐operatively function in the recognition of porphyrin. Copyright © 1999 European Peptide Society and John Wiley&

ISSN:1075-2617    

DOI:10.1002/(SICI)1099-1387(199901)5:1<24::AID-PSC169>3.0.CO;2-L

出版商:John Wiley&Sons, Ltd.    

年代:1999

数据来源: WILEY

摘要:

AbstractA series of peptides derived from sequences from human, bovine, murine and caprine lactoferrin has been prepared and investigated for antibacterial effect. Among the four species investigated peptides based on the bovine sequence displayed significant activity. The bovine sequence, bovine lactoferricin, showed a MIC value of 30 μg/mL onE. coliandS. aureus, whereas the three other lactoferricins possessed MIC values above 200 μg/mL. Based on these findings, novel peptides with enhanced antibacterial activities, were prepared with sequences designed by molecular modelling and structure‐activity studies. Copyright © 1999 European Peptide Society and John Wiley&Sons,

ISSN:1075-2617    

DOI:10.1002/(SICI)1099-1387(199901)5:1<32::AID-PSC172>3.0.CO;2-9

出版商:John Wiley&Sons, Ltd.    

年代:1999

数据来源: WILEY

摘要:

AbstractMultiple antigenic peptides containing dimeric Tn antigen [Ac‐(Tn)2‐γ‐Abu]4‐(Lys‐X)2‐Lys‐β‐Ala (V: X=0;VIII: X=γ‐Abu) and [Ac‐(Tn)2‐γ‐Abu]8‐(Lys‐X)4‐(Lys‐X)2‐Lys‐β‐Ala (XI: X=0;XIV: X=γ‐Abu), immobilized on biocompatible Tenta Gel S NH2support were prepared by SPPS. Rosetting tests ofV,VIII,XIandXIVshowed positive reactions with anti‐Tn (DAKO) and Tn+ erythrocytes, with anti‐Tn/A (BRIC 66) and Tn+ and A erythrocytes, other combinations were negative. In all the animals immunized withXIV, we found a remarkable increase in the level of anti‐Tn (titre 2000–64 000, score 105–167) and no change of anti‐A levels (titre 8, score 13–17). Neither non‐immune nor immune sera showed any reactivity with T+, Cad+and blood group O erythrocytes. Immunized mice did not exhibit any sign of adverse reaction to the administered conjugates. Biological activities were correlated with molecular modelling and molecular dynamic calculations. The biological activities of these synthetic Tn antigen conjugates (good availability for the immunological interactions, highly specific immunogenicity, good biological tolerance) together with their precise chemical characterization seem to be a promising approach to preparation of anti‐tumour vaccine and affinity purification of anti‐Tn antibodies.

ISSN:1075-2617    

DOI:10.1002/(SICI)1099-1387(199901)5:1<46::AID-PSC179>3.0.CO;2-C

出版商:John Wiley&Sons, Ltd.    

年代:1999

数据来源: WILEY

摘要:

AbstractMono‐N‐ethylated α‐amino acid esters are obtained in high yields using reductive amination procedures. Formation of imine is achieved by excess of acetaldehyde, followed by removal of acetaldehyde and reduction by NaBH(OAc)3. The elaborated one‐pot synthesis allows for the efficient synthesis of side‐chain protected amino acid derivatives. Copyright © 1999 European Peptide Society and John Wile

ISSN:1075-2617    

DOI:10.1002/(SICI)1099-1387(199901)5:1<56::AID-PSC186>3.0.CO;2-J

出版商:John Wiley&Sons, Ltd.    

年代:1999

数据来源: WILEY