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1. |
Antibacterial and membrane‐damaging activities ofβ‐bungarotoxin B chain |
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Journal of Peptide Science,
Volume 19,
Issue 1,
2013,
Page 1-8
Yi‐Lin Wen,
Bao‐Jueng Wu,
Pei‐Hsiu Kao,
Yaw‐Syan Fu,
Long‐Sen Chang,
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摘要:
This study investigates whether the B chain ofβ‐bungarotoxin exerted antibacterial activity againstEscherichia coli(Gram‐negative bacteria) andStaphylococcus aureus(Gram‐positive bacteria) via its membrane‐damaging activity. The B chain exhibited a growth inhibition effect onE. colibut did not show a bactericidal effect onS. aureus. The B‐chain bactericidal action onE. colipositively correlated with an increase in membrane permeability in the bacterial cells. Lipopolysaccharide (LPS) layer destabilization and lipoteichoic acid (LTA) biosynthesis inhibition in the cell wall increased the B‐chain bactericidal effect onE. coliandS. aureus. The B chain induced leakage and fusion inE. coliandS. aureusmembrane‐mimicking liposomes. Compared with LPS, LTA notably suppressed the membrane‐damaging activity and fusogenicity of the B chain. The B chain showed similar binding affinity with LPS and LTA, whereas LPS and LTA binding differently induced B‐chain conformational change as evidenced by the circular dichroism spectra. Taken together, our data indicate that the antibacterial action of the B chain is related to its ability to induce membrane permeability and suggest that the LPS‐induced and LTA‐induced B‐chain conformational change differently affects the bactericidal action of the B chain. Copyright © 2012 European P
ISSN:1075-2617
DOI:10.1002/psc.2463
年代:2013
数据来源: WILEY
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2. |
Solid phase synthesis of peptide hydroxamic acids on poly(ethylene glycol)‐based support |
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Journal of Peptide Science,
Volume 19,
Issue 1,
2013,
Page 9-15
Marta Cal,
Mariusz Jaremko,
Łukasz Jaremko,
Piotr Stefanowicz,
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摘要:
A novel resin designed for solid‐phase synthesis of peptide hydroxamic acids (PHA) combining the trityl linker with poly(ethylene glycol)‐based support, ChemMatrix® type, is described. The synthesis of PHA can be performed according to a standard protocol, providing products in excellent purity and reasonable yields. Copyright © 2012 European Peptide Society and John Wiley&Sons
ISSN:1075-2617
DOI:10.1002/psc.2466
年代:2013
数据来源: WILEY
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3. |
Oxidative folding and preparation of α‐conotoxins for use in high‐throughput structure–activity relationship studies |
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Journal of Peptide Science,
Volume 19,
Issue 1,
2013,
Page 16-24
Reena Gyanda,
Jayati Banerjee,
Yi‐Pin Chang,
Angela M. Phillips,
Lawrence Toll,
Christopher J. Armishaw,
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摘要:
α‐Conotoxins are peptide neurotoxins that selectively inhibit various subtypes of nicotinic acetylcholine receptors. They are important research tools for studying numerous pharmacological disorders, with profound potential for developing drug leads for treating pain, tobacco addiction, and other conditions. They are characterized by the presence of two disulfide bonds connected in a globular arrangement, which stabilizes a bioactive helical conformation. Despite extensive structure–activity relationship studies that have produced α‐conotoxin analogs with increased potency and selectivity towards specific nicotinic acetylcholine receptor subtypes, the efficient production of diversity‐oriented α‐conotoxin combinatorial libraries has been limited by inefficient folding and purification procedures. We have investigated the optimized conditions for the reliable folding of α‐conotoxins using simplified oxidation procedures for use in the accelerated production of synthetic combinatorial libraries of α‐conotoxins. To this end, the effect of co‐solvent, redox reagents, pH, and temperature on the proportion of disulfide bond isomers was determined for α‐conotoxins exhibiting commonly known Cys loop spacing frameworks. In addition, we have developed high‐throughput ‘semi‐purification’ methods for the quick and efficient parallel preparation of α‐conotoxin libraries for use in accelerated structure–activity relationship studies. Our simplified procedures represent an effective strategy for the preparation of large arrays of correctly folded α‐conotoxin analogs and permit the rapid identification of active hits directly from high‐throughput pharmacological screening assays. Copyright © 2012 Europea
ISSN:1075-2617
DOI:10.1002/psc.2467
年代:2013
数据来源: WILEY
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4. |
Evaluation of new immunological targets in neuromyelitis optica |
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Journal of Peptide Science,
Volume 19,
Issue 1,
2013,
Page 25-32
Jean‐Baptiste Chanson,
Ilaria Paolini,
Nicolas Collongues,
Maria C. Alcaro,
Frédéric Blanc,
Francesca Barbetti,
Marie Fleury,
Elisa Peroni,
Paolo Rovero,
Gabrielle Rudolf,
Francesco Lolli,
Élisabeth Trifilieff,
Anna‐Maria Papini,
Jérôme Seze,
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摘要:
The detection of reactivity against autoantigens plays a crucial role in the diagnosis of autoimmune diseases. However, only a few autoantibodies are known in each disease, and their precise targets are often not precisely defined. In neuromyelitis optica (NMO), an autoimmune disease of the central nervous system, anti‐aquaporin 4 antibodies are currently the only available immunological markers, although they are not detected in 10–50% of patients. Using enzyme‐linked immunosorbent assays, we evaluated the reactivity against 19 structurally defined peptides in 26 NMO sera compared with 21 healthy subjects. We observed increased levels of IgG against myelin basic protein sequence MBP(156–175), pyruvate dehydrogenase sequence PDH(167–186) and CSF114(Glc), the last of these having a possible correlation with onset of inflammatory relapse. These preliminary results may suggest that the aquaporin 4 is not the unique target in NMO and that the study of reactivity against these peptides would be helpful for the diagnosis and follow‐up of the disease. Complementary studies are however warranted to confirm these results. Copyright © 2012 European Peptide Society and John Wil
ISSN:1075-2617
DOI:10.1002/psc.2470
年代:2013
数据来源: WILEY
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5. |
NMR investigations of structural and dynamics features of natively unstructured drug peptide – salmon calcitonin: implication to rational design of potent sCT analogs |
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Journal of Peptide Science,
Volume 19,
Issue 1,
2013,
Page 33-45
Atul Rawat,
Dinesh Kumar,
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摘要:
Backbone dynamics and conformational properties of drug peptide salmon calcitonin have been studied in aqueous solution using nuclear magnetic resonance (NMR). Although salmon calcitonin (sCT) is largely unfolded in solution (as has been reported in several circular dichroism studies), the secondary Hαchemical shifts and three bond HN–Hαcoupling constants indicated that most of the residues of the peptide are populating the α‐helical region of the Ramachandran (ϕ, ψ) map. Further, the peptide in solution has been found to exhibit multiple conformational states exchanging slowly on the NMR timescale (102–103 s−1), inferred by the multiple chemical shift assignments in the region Leu4–Leu12 and around Pro23 (for residues Gln20–Tyr22 and Arg24). Possibly, these slowly exchanging multiple conformational states might inhibit symmetric self‐association of the peptide and, in part, may account for its reduced aggregation propensity compared with human calcitonin (which lacks this property). The15N NMR‐relaxation data revealed (i) the presence of slow (microsecond‐to‐millisecond) timescale dynamics in the N‐terminal region (Cys1–Ser5) and core residues His17 and Asn26 and (ii) the presence of high frequency (nanosecond‐to‐picosecond) motions in the C‐terminal arm. Put together, the various results suggested that (i) the flexible C‐terminal of sCT (from Thr25–Thr31) is involved in identification of specific target receptors, (ii) whereas the N‐terminal of sCT (from Cys1–Gln20) in solution – exhibiting significant amount of conformational plasticity and strong bias towards biologically active α‐helical structure – facilitates favorable conformational adaptations while interacting with the intermembrane domains of these target receptors. Thus, we believe that the structural and dynamics features of sCT presented here will be useful guiding attributes for the rational design of biologically active sCT analogs. Copyright © 20
ISSN:1075-2617
DOI:10.1002/psc.2471
年代:2013
数据来源: WILEY
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6. |
Investigation on chemotactic drug targeting (chemotaxis and adhesion) inducer effect of GnRH‐III derivatives inTetrahymenaand human leukemia cell line |
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Journal of Peptide Science,
Volume 19,
Issue 1,
2013,
Page 46-58
Eszter Lajkó,
Ildikó Szabó,
Katalin Andódy,
András Pungor,
Gábor Mező,
László Kőhidai,
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摘要:
GnRH‐III has been shown to exert a cytotoxic effect on the GnRH‐R positive tumor cells. The chemotactic drug targeting (CDT) represents a new way for drug delivery approach based on selective chemoattractant guided targeting. The major goal of the present work was to develop and investigate various GnRH‐III derivatives as potential targeting moieties for CDT. The cell physiological effects (chemotaxis, adhesion, and signaling) induced by three native GnRHs (hGnRH‐I, cGnRH‐II, and lGnRH‐III) and nine GnRH‐III derivatives were evaluated in two model cells (Tetrahymena pyriformisand Mono Mac 6 human monocytes). According to our results, the native GnRH‐III elicited the highest chemoattractant and adhesion inducer activities of all synthesized peptides in micromolar concentrations in monocytes. With respect to chemoattraction, dimeric derivatives linked by a disulfide bridge ([GnRH‐III(C)]2) proved to be efficient in both model cells; furthermore, acetylation of the linker region ([GnRH‐III(Ac‐C)]2) could slightly improve the chemotactic and adhesion effects in monocytes. The length of the peptide and the type of N‐terminal amino acid could also determine the chemotactic and adhesion modulation potency of each fragment. The application of the chemoattractant GnRH‐III derivatives was accompanied by a significant activation of phosphatidylinositol 3‐kinase in both model cells. In summary, our work on low‐level differentiated model cells of tumors has proved that GnRH‐III and some of its synthetic derivatives are promising candidates to be applied in CDT: these compounds might act both as carrier, delivery unit, and antitumor agents. Copyright © 2012 European Peptide
ISSN:1075-2617
DOI:10.1002/psc.2472
年代:2013
数据来源: WILEY
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7. |
Development and clinical application of an enzyme immunoassay for the determination of midregional proadrenomedullin |
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Journal of Peptide Science,
Volume 19,
Issue 1,
2013,
Page 59-63
Yosuke Suzuki,
Hiroki Itoh,
Fumihiko Katagiri,
Fuminori Sato,
Yukie Sato,
Kanako Kawasaki,
Yuhki Sato,
Hiromitsu Mimata,
Masaharu Takeyama,
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摘要:
Adrenomedullin (ADM) is a 52‐amino acid peptide with a variety of physiologic functions such as immunomodulating activity, direct bactericidal activity, maintenance of renal homeostasis, and vasodilatory activity. Midregional proADM (MR‐proADM) is derived from a larger 185‐amino acid precursor peptide, prepro‐adrenomedulin (preproADM), by posttranslational processing. It is suggested to be co‐synthesized with ADM in equimolar amounts and has the advantages over ADM in having a longer half‐life, no bioactivity, and no binding to protein. Therefore, MR‐proADM serves as a surrogate for ADM secretion. In this study, we attempted to develop an enzyme immunoassay (EIA) for quantifying MR‐proADM‐like immunoreactive substance (IS), which is applicable for monitoring plasma MR‐proADM levels. By usingβ‐d‐galactosidase‐labeled preproADM(83‐94) as a marker antigen, anti‐rabbit IgG‐coated immunoplate as a bound/free separator, and 4‐methylumbelliferyl‐β‐d‐galactopyranoside as a fluorogenic substrate, a sensitive and specific EIA was developed for the quantification of MR‐proADM‐IS in human plasma. The lower limit of quantification was 0.032 pmol/well, and the steep competitive inhibition EIA calibration curve obtained was linear between 0.16 and 10 nmol/L. By using human plasma samples containing 0.2 and 2.0 nmol/L of MR‐proADM, the interassay coefficients of variation (reproducibility) were 10.78% and 8.83%, respectively, and intraassay coefficients were 3.91% and 7.81%. Plasma MR‐proADM‐IS level was significantly higher in patients with chronic renal failure (1.39 ± 0.50 nmol/L) compared with healthy subjects (0.19 ± 0.07 nmol/L). These results suggest that our EIA may be useful to evaluate plasma MR‐proADM levels as a biomarker in various clinical set
ISSN:1075-2617
DOI:10.1002/psc.2473
年代:2013
数据来源: WILEY
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