摘要:

Using voltage-clamp techniques, we have examined embryonic sodium currents in neurons deficient for a gene located at 60E5/6 that shares extensive amino acid similarity with vertebrate sodium channel genes. These neurons expressed sodium currents similar to wildtype, supporting the hypothesis thatpara, and not the gene at 60E5/6, is the primary sodium channel gene expressed in embryonic neurons. A simple marking procedure allowing positive identification of the genotypes of culturedDrosophilaembryos obtained from heterozygous parents was used to recognize cultures homozygous for deficiencies. The morphological development of both neurons and myotubes in these cultures was similar to wildtype, making it feasible to compare the properties of normal diploid cells and cells completely lacking a putative sodium channel gene.

ISSN:0167-7063    

DOI:10.3109/01677069209167268

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

otal(ota = olfactory trap abnormal), an X-linked mutation ofDrosophilaisolated by virtue of abnormal olfactory behavior, is shown to be an allele ofrdgB(retinal degeneration B), a gene required for normal visual system physiology.rdgBfunction is shown to be necessary for olfactory response of both adult flies and larvae, which have distinct olfactory systems. Electrophysiological recordings from the adult antenna indicate thatrdgBis required for normal response in the peripheral olfactory system: somerdgBmutants show a delayed return to the resting potential following stimulation with ethyl acetate vapor. These results indicate thatrdgBis required for both visual and olfactory physiology, and they suggest that visual and olfactory transduction may share at least one common molecular step inDrosophila.

ISSN:0167-7063    

DOI:10.3109/01677069209167269

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

Cell bodies in the rat suprachiasmatic nucleus (SCN) were labeled with an antibody against a small domain of theperiod(per) protein, the product of a gene inDrosophilathat regulates circadian rhythms. In immunoblots of SCN protein extracts, the antibody recognized a band of approximately 115 kD, as well as a heterogeneous antigen ranging from 160 kD to 170 kD. The antibody was found in previous studies to label putative circadian pacemaker neurons inAplysiaandBulla, as well as the cellular sites ofperexpression in flies. Taken together, these results suggest that the region of theperprotein recognized by this antibody may be widely conserved in neuronal circadian pacemakers.

ISSN:0167-7063    

DOI:10.3109/01677069209167270

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor

摘要:

Mutations in the complexoptomotor-blind (omb)gene locus (4C4-6) lead to a number of different phenotypes in various tissues of the adultDrosophila melanogasterfly. At the core of the locus lies a lethal complementation group, namedl(1)omb, whose mutations cause larval and pupal lethality. Some 40% of all males hemizygous for lethalomballeles develop to the pharate adult stage. These flies can be rescued from the pupal case and show a severe disturbance in optic lobe development. The recessive viable alleleIn(1)ombH31reduces the optomotor response in walking flies and during stationary flight of tethered flies. At the neuroanatomical level, these animals lack a subset of lobula plate giant neurons (LPGNs), which are thought to mediate optomotor behavior. Chromosomal aberrations deleting the proximal, non transcribed part of the locus complement the lethality, but still cause neuroanatomical and optomotor defects. Analysis of different allelic combinations of such mutations, in which increasing amounts of DNA downstream of the transcribed region are removed, reveals a step gradient of increasing severity of the neuroanatomical defects and behavioral phenotypes. On this basis the 3′-regulatory region is divided into three domains each having specific effects on optic lobe development.

ISSN:0167-7063    

DOI:10.3109/01677069209167271

出版商:Taylor&Francis    

年代:1992

数据来源: Taylor