摘要:

We have reintroduced an 8 kb genomic fragment from theDrosophilatyrosine hydroxylase (DTH) locus into the genome of mutantpale (ple)flies.plewas first recovered as a recessive embryonic lethal by Jurgenset al.(1984) and maps to the same chromosomal region as DTH (65A-E). Mutantplealleles affect pigmentation of the cuticle (L-DOPA, the product of the reaction catalyzed by TH, is an intermediate in the cuticular sclerotization and pigmentation pathways) and catecholamine biosynthesis. In this report we demonstrate that ple does encode the structural gene for TH, since the reintroduced sequences rescuepleflies from lethality to viable adults. Morphological, immunocytochemical, and behavioral characterization of three transformant lines suggests that the reintroduced sequences contain the necessary elements for correct temporal and spatial expression of the gene, but may not contain all the sequences essential for quantitative expression.

ISSN:0167-7063    

DOI:10.3109/01677069309083448

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

摘要:

bendless-(ben-) is an X chromosome mutation inDrosophila melanogaster, known to alter patterns of connections in the CNS and thus modify behavior (Thomas and Wyman, 1984). We report that in addition to its CNS effects,ben-has pleiotropic phenotypes affecting thoracic muscle patterning, pupal mortality, and post-eclosional mobility. The tergal depressor of the trochanter (TDT) normally attaches ventrally to an apodeme on the trochanter and dorsally to the lateral scutum just posterior to the intras-cutal suture. Inben-individuals, TDT may attach anywhere within the boundaries of the attachment areas for TDT and dorsoventral muscles I (DVM I) and II (DVM II). Furthermore, TDT may completely lack a dorsal attachment, although it still maintains a ventral attachment. DVMs may also attach abnormally to dorsal sites normally occupied by an adjacent DVM, or may be entirely eliminated. DVM loss occurs independently of the position or presence of TDT dorsal attachment. The cytology ofben-TDT is altered. Muscles may have fibers that are swollen and stain abnormally. Other fibers may have large, axially aligned holes.ben-flies have an increased likelihood of failing to eclose and, upon eclo-sion, show impaired mobility. We describe several possible mechanisms for theben-developmental defects and discuss this mutation in light of its evolutionary significance.

ISSN:0167-7063    

DOI:10.3109/01677069309083449

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor

ISSN:0167-7063    

DOI:10.3109/01677069309083450

出版商:Taylor&Francis    

年代:1993

数据来源: Taylor