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1. |
Sterilization with gaseous ethylene oxide: A review of chemical and physical factors |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 1-31
Robert R. Ernst,
John E. Doyle,
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摘要:
AbstractAlthough the basic parameters for ethylene oxide sterilization are established, it is sometimes difficult to attain in practice where the principal limiting factor is moisture availability. There are situations which can limit or enhance the dynamics of sterilization. Such factors, if overlooked, could upset experiments and lead to erroneous conclusions, or defeat the sterilization process entirety. Such are, namely: stratification effects, diffusion barriers, moisture‐reducing effects, polymerization, and temperature distribution gradient
ISSN:0006-3592
DOI:10.1002/bit.260100102
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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2. |
Cultivation of the yeastcandida lipolyticaon hydrocarbons. I. Degradation ofn‐alkanes in batch fermentation of gas oil |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 33-43
M. Dostálek,
V. Munk,
Olga Volfová,
K. Pecka,
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摘要:
AbstractThe growth of batch‐cultivated yeastCandida lipolyticaon three kinds of gas oil using mineral medium was studied. A linear dependence was found between the production of yeast biomass and the consumption ofn‐alkanes, while the decrease of freezing point of gas oil during cultivation had a distinct course. This disproportion was explained by different degradation of individualn‐alkanes contained in gas oil. The rate of degradation of pentadecane, hexadecane, and heptadecane was the same during the entire cultivation. On the contrary, in the first phase the utilization of shorter chainn‐alkanes, nonane to tetradecane, was more rapid while that of longer chain homologs, octadecane to pentacosane, lagged. Rapid utilization of longer chainn‐alkanes did not occur before the concentration of the othern‐alkanes decreased. Only then the rapid decrease of freezing poi
ISSN:0006-3592
DOI:10.1002/bit.260100103
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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3. |
The role of aeration and agitation in the production of glucose oxidase in submerged culture |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 45-59
Kornelia Zetelaki,
Karoly Vas,
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摘要:
AbstractThe influence of agitation and aeration on growth and on production of glucose oxidase ofAsp. nigerhas been studied. It was found that both rate of growth and glucose oxidase production was higher at an agitation speed of 700 rpm than at 460 rpm. Further increase in speed of agitation resulted in neither a higher rate of growth nor a higher glucose oxidase activity. Total glucose oxidase activity was highest in a medium containing 5% sugar (at an agitation speed of 700 rpm) and did not get higher when the sugar concentration of the medium was increased to 7%. When pure oxygen was bubbled through the culture the rate of growth of the culture (in the linear phase) was 95 mg. mycelial dry wt./100 ml./hr., and only 61 mg. when air was applied. The glucose oxidase activity of oxygenated culture was double the activity of aerated culture. Viscosity of the homogenized culture became higher with higher concentration of mycelia. The viscosity of oxygenated culture was found to be lower than that of aerated culture.
ISSN:0006-3592
DOI:10.1002/bit.260100104
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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4. |
Growth of mouse fibroblast‐like cells in an autoclavable medium |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 61-67
M. D. Orlando,
O. C. Bowersox,
J. M. Riley,
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摘要:
AbstractA relatively simple, autoclavable medium was developed that would support the growth of the mouse fibroblast (MFL) cell line in suspension culture. This medium was prepared in three stages with decreasing quantities of serum. As the serum was reduced from 5% to none, the amount of Bacto‐Peptone was increased from none to 0.5%. The reduction and finally elimination of serum did not affect proliferation adversely, but actually seemed to produce more rapid growth and higher levels of cell populatio
ISSN:0006-3592
DOI:10.1002/bit.260100105
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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5. |
A method for determining constants of first‐order reactions from experimental data |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 69-82
W. P. Isaacs,
A. F. Gaudy,
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摘要:
AbstractThe authors have utilized a previously proposed mathematical equation (introduced originally for development of empirical equations) as a useful tool for evaluation of first‐order reaction rate constants. By assigning physical significance to the parameter α, the equation can be utilized in obtaining excellent estimates for limiting boundary values and velocity constan
ISSN:0006-3592
DOI:10.1002/bit.260100106
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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6. |
A mathematical model for the batch reactor kinetics of algae growth |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 83-88
Francis P. Ragonese,
John A. Williams,
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摘要:
AbstractA mathematical model based on the Einstein law of photochemical equivalence is proposed to describe the batch growth of unicellular algae. The model was applied in an integrated form to cell concentration versus growth time data taken over an extended range of cell concentrations which include both the regions of “exponential” and “linear” growth. It is shown that a certain function of cell concentration contained in the integrated form of the model is linearly dependent on the growth time over both the “exponential” and “linear”
ISSN:0006-3592
DOI:10.1002/bit.260100107
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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7. |
Microflotation: Coagulation and foam separation ofaerobacter aerogenes |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 89-98
Alan J. Rubin,
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摘要:
AbstractThe foam separation technique of microflotation was studied usingA. aerogenesas the test organisms. Gas flow rate, collector and alum (when used) concentrations, and frother dose were held constant. In contrast toE. coli, previously reported,A. aerogenesare removed using both lauric acid and laurylamine as the collectors without prior coagulation with alum. The removals are improved after coagulation, with laurylamine being the more efficient collector. In all cases the removals decrease upon increasing the pH above about 8.
ISSN:0006-3592
DOI:10.1002/bit.260100108
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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8. |
Frequency response analysis of dilution rate effects on yeast growth |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 99-101
J. W. Gilley,
H. R. Bungay,
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ISSN:0006-3592
DOI:10.1002/bit.260100109
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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9. |
Effect of glycols on the production of ergot alkaloids byClaviceps paspali |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page 102-103
A. Mizrahi,
G. Miller,
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PDF (74KB)
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ISSN:0006-3592
DOI:10.1002/bit.260100110
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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10. |
Masthead |
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Biotechnology and Bioengineering,
Volume 10,
Issue 1,
1968,
Page -
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PDF (66KB)
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ISSN:0006-3592
DOI:10.1002/bit.260100101
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1968
数据来源: WILEY
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